Journal of Knowledge & Health1735-577X14120190618The Effect of an Interval Exercise Period (HIIT) on MTNR1B Gene Expression, Insulin and Glucose Levels in Type 2 Diabetic Rats283510.22100/jkh.v14i1.2151FAMohammadORCID:0000-0002-2675-9539Rashidi11- Dept. of Exercise Physiology, Semnan Branch, Islamic Azad University, Semnan, Iran.. mrashidi48@yahoo.comMojtabaORCID:0000- 0003-1989-692xEizadi22019011920190517Introduction: MTNR1B genes expression is associated with increase of the of type 2 diabetes . The objective of this study was to evaluate the effect of 12 weeks of high intensity intermittent exercise on MTNR1B gene, glucose and insulin levels in diabetic rats Methods: In this study, 30 male Wistar rats weighing 220± 20g were divided into three groups control, diabetic and HIIT/diabetic groups. Administration of streptozotocin (STZ) and nicotinamide (NA) induced experimental diabetes in the rat. Interval Exercise (HIIT) performed for 12 weeks (5 times/30 mintes). The glucose concentration examined using oxidase colorimetric method, insulin by ELISA and gene expression was measured by RT-Real time PCR. All statistical analysis performed using s SPSS.Results: Exercise program decreased fasting blood glucose and serum insulin in diabetic group compared to the control (P=0.001). Intensive interval training decreased MTNR1B gene expression in pancreatic tissue compared to diabetic control group (P=0.023). There was a significant correlation between the expression of MTNR1B and serum insulin (P=0.039, r=-0.84)Conclusion: The results showed that twelve weeks of high intensity intermittent exercise decrease blood glucose and increase serum insulin and decrease of MTNR1B gene expression in pancreatic tissue.