Journal of Knowledge & Health1735-577X14220191001Supplementary Containing Vit E, C and Se Reduced the PCOs Induced Oxidative Stress in Rat Model2260226010.22100/jkh.v14i2.2260FAFereshtehMansouri Nezad11- Biolgy (Mirobiology), Azad University, Zanjan Branch, Zanjan, Iran.. email@example.comMaryamNaghipour Hamzekolaei2MoslemJafariani3*ImanSadeghi4201906192019092920190929Introduction: The most common endocrine disorder in women of infertility is polycystic ovary syndrome. Increased oxidative stress is one of the pathogenesis of this disease. Therefore, combating oxidative stress is helpful in reducing the complications of this disease. In the present study, the effects of vitamin E, C and selenium supplementation on the induction of polycystic ovary induced rat model were investigated.Methods: Thirty female Wistar rats were divided randomly into 6 groups. Induction of polycystic ovary syndrome was performed with letrozole gavage. The groups were then treated with vitamin C, vitamin E, selenium, and a mixture of vitamins C, E, and Se for one month. The control group received normal saline during the study period. The mice were sacrificed after one month and their blood was taken for oxidative stress tests including total antioxidant capacity and free oxygen species and malondialdehyde. Their ovaries were also removed for pathological examination and transferred to formalin.Results: The results showed that there was an increase in oxidative stress in the polycystic ovary induction group (P<0.05). The results also showed that the group with the simultaneous supplementation of vitamin C, E, Se and Se had the highest decrease in oxidative stress indices (P<0.003). Also vitamin E group showed better improvement compared to vitamin C and Se.Conclusion: Antioxidants are used to prevent the increase of oxidative stress, but their simultaneous use has synergistic effects.