Journal of Knowledge & Health in Basic Medical Sciences1735-577X12420180317The Effect of Four-Week Aerobic Activity in Water on Clinical Signs and TNF-α and IL-10 Cytokines in Animal Model of Multiple Sclerosis (MS)476010.22100/jkh.v12i4.1818FAMaryamVatandoust1*1. Dept. of Sport Physiology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran.. maryam.vatandost@gmail.comPezhmanMotamedi2HamidRajabi3Ata AllahGhadiri4AbdolmahdiNasirzade5FatemeVatandoust6EnayatoallahYazdanpanah7201711152018011720180107Introduction: According to importance of complimentary therapies, The aim of this study was to evaluate the effect of four-week aerobic physical activity in water on clinical signs and amount of Tumor Necrosis Factor alpha (TNF- α) and Interleukin 10 (IL-10) in serum levels of animal model of multiple sclerosis (MS) via inducing experimental autoimmune encephalomyelitis (EAE).Methods: A total number of 80 female Syrian mice from the race of C57BL/6, aging 10 to 12 weeks and weighing 20±2 gram were divided into eight groups of 10, including: control, swimming, MS, MS+swimming, MS+interferon beta (INF- α), MS+interferon beta+swimming, MS+solvent, and MS+solvent+swimming environment. On post-immunization day 9, animals received IFN (150 IU/g) or were subjected to swimming daily for 4 weeks. Weight and clinical score were monitored daily and recorded in all groups. Finally blood samples were taken from the heart and the factors were measured by ELISA. The data were analyzed by one-way ANOVA.Results: According to clinical signs diagrams, there was seen significant difference between MS+swimming, MS+interferon beta, MS+interferon beta+swimming with MS group (P=0.001). Also, Based on the scores of clinical signs, the severity of injury in MS groups compared to active subjects had a average score of 2 to 3, indicating control and reduction of the progression of demyelin lesions resulting from the effect of exercise in water.Conclusion: These findings suggest that probably swimming is more effective than IFN to alter clinical signs and the level of TNF- α and IL-10 in the serum of EAE-induced mice. Therefore we conclude that regular moderate exercise could be helpful beside the medical therapies to attenuate the MS disorders.