Journal of Knowledge & Health1735-577X13220180912The Simultaneous Effect of Regular Exercise and Vitamin D on Apoptosis Level and Antioxidant Enzymes of Heart Tissue of Male Rats Exposed to Oxygenated Water294110.22100/jkh.v13i2.1935FAMehdiPirooz 1Dept. of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.. mehdipirooz@yahoo.comMohammad AliORCID: 0000-0002-3502-7487Azarbayjani 1Seyed AliHosseini 2MaghsoudPeeri 12018051420180527Introduction: Biological effects of strong oxidizing compounds in the human body are controlled by antioxidant agents. Disorders of the organs may be result of reactions between free radicals and cells membrane. It has been shown that the main objective of the oxygen radicals is the lipids cell membrane. The aim of this study was to evaluate the myocardial cellular vulnerability and the evaluation of antioxidant enzymes in the heart tissue of male rats.Methods: Sixty adult male wistar rats, 220 ± 220 gram and 8-10 weeks years old, were randomly assigned into 10 groups of six by considering stress, vitamin D and regular exercise activities. Groups were control, water Oxygenated water H2O2 (H); twice Oxygen water of 2H2O2 (2H), Oxygen water + Vitamin D (HD), Oxygen water doubles + Vitamin D (2HD), Oxygenated water + Exercise (HE), Oxygenated water twice + Exercise (2HE)), Oxygenated Water + Exercise + Vitamin D (HDE), Oxygenated Water + Double Exercise + Vitamin D (2HDE) and finally Diethyl Sulfoxide + Saline (DMSO) were subjected to an intervention protocol for 8 weeks. Then, the concentration of antioxidant enzymes in measuring heart tissue analysis of variance was analyzed by the three-way anova. The significance level was set at 0.05.Results: The results showed that exercise increased SOD and GPX, but catalase increase was not significant. Also, exercise interaction with vitamin D also increased the antioxidant enzymes, but these enzymes did not increase in groups which did not complete the exercise. On the other hand the concentration of malondialdehyde MDA increased in these groups. The combination of exercise, vitamin D and oxygenated water has a synergistic effect on inhibiting the activity of the FAS protein, and is effective in protect and controlling the complications of apoptosis and myocardial inflammation.Conclusion: Concerning the use of different doses of H2O2 in comparison with the training group and D alone or in the control group, it was shown that the combination of endurance training and D increased significantly the activity of SOD and GPX enzymes.