Physicochemical Characterization of Chitosan-Filled Nanocarriers of Beta-Cyclodextrin / Mesalazine: Drug Release Process and Enhancement of Antioxidant Properties

Abstract

Introduction: Mesalazine (MSZ, 5-amino-2-hydroxybenzoic acid i

s an anti-inflammatory drug that is commonly used to treat mild to moderately active ulcerative colitis,This study aimed to develop a novel sustained release system for mesalazine (MSZ) by preparing β-cyclodextrin (β-CD) inclusion complex loaded chitosan (CS) nanoparticles (NPs).

Methods: Complexes of MSZ with modified β-CD was prepared by chitosan nanoparticles (CS). and characterized by using UV-Vis, FT-IR, SEM and 1HNMR. Calculations of the relationships of the formation of modified complexes and their application were performed using UV-vis spectroscopic data analysis.

Results: Phase-solubility analysis showed AL-type diagram with beta-CD,. according to the Higuchi and Connors method, is Kstab = 696.462 M−1. The in vitro tests showed that the formation of the inclusion complex  increase in the 2,2՜-diphenyl-1-picrylhydrazyl (DPPH˙) radical-scavenging capacity  compared to the free MSZ at the same concentration.

Conclusion: The results revealed that Under the optimized parameters, the resultant MSZ/β-CD-CS NPs were found to be nano-spheres with small particle size and high drug loading efficiency. Conclusively, the MSZ/β-CD-CS NPs exhibited vast potential for developing novel delivery system of MSZ with a sustainedrelease profile.