Antibacterial Effects of Hydroalcoholic Extract of Flower Rosa Foetida Herm on Gram Positive And Gram Negative Bacteria
Authors
-
Zahra Moulana
- Infectious and Tropical Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
https://orcid.org/0000-0002-7072-8182
- Javid Mohseni - Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
-
Fariba Asgharpour
- Dept. of Laboratory Sciences, Faculty of Para- Medicine, Babol University of Medical Sciences, Babol, Iran.
https://orcid.org/0000-0001-6128-5407
-
Parvin Sajadi Kaboudi
- Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
https://orcid.org/0000-0003-1655-8909
DOI:
https://doi.org/10.22100/jkh.v19i1.3180Keywords:
Extract, Flower, Rosa foetida, MIC, MBCAbstract
Introduction: The rise in bacterial resistance to antibiotics and their negative side effects has led to a growing interest in the use of medicinal plants and plant extracts. This study aimed to investigate the antibacterial effects of hydroalcoholic extract of Yellow Nasturtium on some gram-positive and gram-negative bacteria.
Methods: In this research, after the preparation of Rosa Foetida extract, its antibacterial properties were evaluated using antibiogram, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. Ciprofloxacin, gentamicin, and penicillin were used to determine the sensitivity of bacteria to the extract studied.
Results: The results showed that the hydroalcoholic extract of Rosa Foetida effectively inhibited bacterial growth and viability, with a more pronounced antibacterial effect on gram-positive bacteria than on gram-negative bacteria. Using the MIC method, the extract was able to inhibit the growth of bacteria with an average of 12.5-100 mg/ml. The results of the study compared to CLSI 2018 showed that only Escherichia coli and Klebsiella pneumoniae bacteria were sensitive to ciprofloxacin and all bacteria were resistant to gentamicin and penicillin antibiotics. The results of the one-way analysis of variance revealed that there was a significant difference between the effect of extract or antibiotics against bacteria (P<0.01).
Conclusion: Collectively, hydroalcoholic extract of Yellow Nasturtium could be a valuable source of antibacterial activity against certain gram-positive and gram-negative bacteria. Natural antibacterial compounds are efficient for utilization in in vitro phytochemical and biological investigations.
References
Halder S, Anand U, Nandy S, Oleksak P, Qusti S, Alshammari EM, et al. Herbal drugs and natural bioactive products as potential therapeutics: A review on pro-cognitives and brain boosters perspectives. Saudi Pharm J 2021;29:879-907. doi: 10.1016/j.jsps.2021.07.003
Karar MG, Kuhnert N. Herbal drugs from Sudan: Traditional uses and phytoconstituents. Pharmacogn Rev 2017;11:83. doi: 10.4103/phrev.phrev_15_15
Zheng J, Fan R, Wu H, Yao H, Yan Y, Liu J, et al. Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation. Nature Communications 2019;10:1-2. doi: 10.1038/s41467-019-09601-3
Quiñonez-Bastidas GN, Navarrete A. Mexican Plants and Derivates Compounds as Alternative for Inflammatory and Neuropathic Pain Treatment-A Review. Plants 2021;10:865. doi: 10.3390/plants10050865
Wuli W, Tsai ST, Chiou TW, Harn HJ. Human-induced pluripotent stem cells and herbal small-molecule drugs for treatment of Alzheimer’s disease. Int J Mol Sci 2020;21:1327. doi: 10.3390/ijms21041327
Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH, et al. Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist 2018;11:1645. doi: 10.2147/IDR.S173867
Arjmandi AA, Sharghi HR, Memariani F, Joharchi MR. Rosa kokanica (Rosaceae) in Binalood Mountains: A new record for the flora of Iran. IRAN J BOT 2016;22:11-5. doi: 10.22092/IJB.2016.106629
Samiei L, Naderi R, Khalighi A, Bushehri AA, MozaffarianV, Esselink D, et al. In search of genetic diversity in rosa foetida herrmann in Iran. ActaHortic 2009;836.3. doi: 10.17660/ActaHortic.2009.836.3
Shabani F, Omidvar SH, Sajadi Kaboudi P, Pasha H, Khafri S, Najafzadehvarzi H, et al. The effects of Rosa foetida extract along with self-care education on primary dysmenorrhea: study protocol for a randomized clinical trial. Trials 2022;623:637. doi: 10.1186/s13063-022-06583-4
Rezghi M, Hoseinidoust SR, Asgarpanah J. Rosa foetida Herrm. flowers as a future natural antibacterial agent against the main cause of skin burn wound infections, Pseudomonas aeroginosa. Journal of Herbal Drugs 2015;5:209-13.
Akhoondi R, Mirjalili MH, Hadian J. Quantitative and qualitative variations in the essential oil of Rosa foetida Herrm.(Rosaceae) flowers as affected by different drying methods. Journal of Essential Oil Research 2015;27:421-7.
Ziarati P, Asgarpanah J, Safialdinardebilya M. The volatile oil composition of Rosa foetida Herrm. flowers growing wild in Kurdistan province (Iran). J Essent Oil-Bear Plants 2014;17:169-72. doi: 10.1080/0972060X.2014.884765
Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI methods development and standardization working group best practices for evaluation of antimicrobial susceptibility tests. J Clin Microbiol 2018;56:e01934-17. doi: 10.1128/JCM.01934-17
Hakemi VM, Mojab F, Mahbobi L, Bagheri BF. Identification of aromatic components of rosa foetida hermm (persion yellow rose) extract and its antiacterial effect. Iranian Congress of Physiology and Pharmacology 2009.
Rovná K, Petrová J, Terentjeva M, Černá J, Kačániová M. Antimicrobial activity of Rosa canina flowers against selected microorganisms. J Microbiol Biotech Food Sci 2021;2021:62-4. doi: 10.15414/jmbfs.2015.4.special1.62-64
Quave CL, Plano LR, Pantuso T, Bennett BC. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol 2008;118:418-28. doi: 10.1016/j.jep.2008.05.005
Serteser A, Kargioğlu M, Gök V, Bağci Y, Özcan MM, Arslan D. Determination of antioxidant effects of some plant species wild growing in Turkey. Int J Food Sci Nutr 2008;59:643-51. doi: 10.1080/09637480701602530
Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 2003;74:394-6. doi: 10.1016/s0367-326x(03)00044-3
Zhang W, Abdel-Rahman FH, Saleh MA. Natural resistance of rose petals to microbial attack. J Environ Sci Health B 2011;46:381-93. doi: 10.1080/03601234.2011.572502
Sajadi Kabodi P, Bakhshi D, Moghadamnia Aa, Sefidgar Aa. The Antibacterial Effects of Methanol Extract of Ammi majus on Staphylococcus aureus and Escherichia coli. Journal of J Babol Univ Med Sci 2017;19:36-42. doi: 10.22088/jbums.19.1.36
Kiehlbauch JA, Hannett GE, Salfinger M, Archinal W, Monserrat C, Carlyn C. Use of the National Committee for Clinical Laboratory Standards guidelines for disk diffusion susceptibility testing in New York state laboratories. J Clin Microbiol 2000;38:3341-8. doi: 10.1128/jcm.38.9.3341-3348.2000
Pokorný J. Are natural antioxidants better–and safer–than synthetic antioxidants?. European Journal of Lipid Science and Technology 2007;109:629-42. doi: 10.1002/ejlt.200700064
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
