Study of the Effect of Cambodian Mushroom Extract and Garlic on Testicular Damage in Diabetic Desert Mice with Streptozotocin
Authors
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Ghazale Yavari
- Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0001-5055-7841
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Sahar Molzemi
- Department of Tissue Engineering and Applied Cell Sciences, Faculty of Modern Medical Technologies, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
https://orcid.org/0000-0003-3069-5926
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Vida Hojati
- Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
https://orcid.org/0000-0002-0408-389X
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Zahra Kordi
- Vice‑chancellery for Research, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0002-7191-6310
DOI:
https://doi.org/10.22100/jkh.v19i1.3229Keywords:
diabetes, garlic, Cambodian fungus, testicular damageAbstract
Introduction: Diabetes causes damage to various tissues, including the testicles. Garlic and Cambodian mushrooms play an important role in preventing diabetes complications in the testicles due to their antioxidant properties. The present study aimed to investigate the effect of Cambodian mushroom extract and garlic on testicular damage in healthy and diabetic desert mice.
Methods: 40 male Wistar rat heads were divided into 5 groups of 8, including control group, negative control group (witness) (diabetic, with mg/kg 55 Streptozotocin intraperitoneal), experimental group 1 (diabetic + mg/kg 50 garlic extract), experimental group 2 (diabetic + 50 mg/kg Cambodian mushroom), and experimental group 3 (diabetic + mg/kg 50 Cambodian mushroom and mg/kg 50 garlic extract). After two months of diabetes, the mice received the extracts subcutaneously for two weeks. The mice were then anesthetized with ketamine and xylazine, and the testes were examined macroscopically and microscopically.
Results: The diabetic group showed a significant decrease in spermatogenic cells compared to the control group. Conversely, the experimental groups showed a significant increase in spermatogenic cells compared to the diabetic group.
Conclusion: The findings of this study demonstrate that prolonged oral intake of garlic and Cambodian extract enhances the secretion of the blood hormones insulin and testosterone, and promotes the spermatogenesis process.
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