Fibroblast Growth Factor in Male Semen and Its Correlation with Sperm Parameters
Authors
- Hamidreza Zahedi - Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
-
Naghmeh Ahmadiankia
- Department of Anatomy and Cellular Biology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.- Cancer Prevention Research Center, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0001-8309-4935
-
Mozhgan Fazli
- Department of Microbiology and Immunology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0002-8703-5446
-
Sara Saeednia
- Department of Anatomy and Cellular Biology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. - Tissue and Cell Engineering Research Center Foundation, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
https://orcid.org/0000-0002-0424-9722
DOI:
https://doi.org/10.22100/jkh.v20i4.3370Keywords:
Fibroblast Growth Factor (FGF), Normozoospermic , Asthenozoospermic , SpermogramAbstract
Introduction: This study aimed to investigate the levels of Fibroblast Growth Factor (FGF), a key mediator of spermatogenesis and sperm motility, in the seminal plasma of normozoospermic and asthenozoospermic individuals residing in Shahroud.
Methods: This work examined 30 semen samples from men aged 20-60 years attending the Mehrshahr Laboratory in Shahroud. Semen analysis was performed using a Computerized Assisted Semen Analyzer (CASA) according to WHO criteria. The samples were accordingly classified into two groups: asthenozoospermic (n=15) and normozoospermic (n=15). The primary parameters assessed included age, seminal fluid volume, sperm concentration, sperm motility, progressive motility, sperm morphology, and FGF concentration.
Results: The results showed that there was no difference in age, semen volume, semen pH, and all qualitative characteristics (e.g., stickiness, agglutination, aggregation, odor, color) between the two groups. There was a significant difference in the number of sperm, motile sperm, progressive motility percentage, and percentage of sperm with normal morphology between normozoospermic group and asthenozoospermic group (P<0.05). Also, FGF concentration in the normozoosperm group was significantly higher than in the asthenozoosperm group (P<0.01). Correlation analysis revealed a direct relationship between increasing FGF concentration and increased percentages of motile sperm (P=0.07), sperm with progressive motility (P=0.08), and sperm count ; however, these correlations did not reach statistical significance.
Conclusion: Significant differences in sperm count, motility, morphology, and FGF concentration were found between the two groups. Beside, FGF concentration revealed a correlation with sperm count and motility, though not always statistically significant.
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