The Effect of Cell Extract of Kefir Microorganisms on the Metastatic Potential of Breast and Glioblastoma Cancer Cell Lines

Abstract

Introduction: Kefir-forming microorganisms can be effective in the prevention and treatment of various types of cancer. The present study aims to investigate the effect of kefir microorganism cell extract on the metastatic potential of breast cancer and glioblastoma cell lines.

Methods: Following cell proliferation, MDA-MB-231 and T98G cell lines were treated with appropriate concentrations of kefir microorganism cell extract, and cell viability was assessed by MTT assay. Subsequently, the extract’s effect on cell migration and invasion capabilities was analyzed.

Results: After 24 hours of treatment with supernatant and cell lysis, a concentration- and time-dependent decrease in cell viability was observed. The IC50 values for 24 and 48 hours were 73.85 and 66.13 mg/ml for supernatant and 102.39 and 75.88 mg/ml for cell lysis for the MDA-MB-231 cell line, respectively. Also, the calculated IC50 for 24 and 48 hours was 24.30 and 10.55 mg/ml for supernatant and 82.53 and 16.23 mg/ml for cell lysate for the T98G cell line, respectively. Moreover, after 24 hours of treatment with IC50 concentrations of supernatant and cell lysate, a significant decrease in the migration and invasion potential of cells was observed (P<0.05).

Conclusion: The results showed that kefir significantly reduced the growth rate and metastasis of glioblastoma and breast cancer cells. As a result, kefir can be an option for cancer treatment.