Assessment of Cytotoxic Activity of Three Plants of Pigweed, Bermuda grass and Burdock via Artemia Salina Test
DOI:
https://doi.org/10.22100/jkh.v5i2-3.136Keywords:
Artemia salina, Toxicity test, Plants.Abstract
Introduction: Pigweed is a weed which grows in the pastures of Iran. Local people use the plant leaves to cook traditional food. Bermuda grass with its rapid growth and distribution is regarded a real threat to prairies and it can lead to tremors syndrome in cattle and sheep. Burdock which also grows in many parts of Iran is used as a medicinal herb. In this study, the cytotoxicity potential of the three plants was assessed via Artemia Salina test.
Methods: Pigweed, Bermuda grass and burdock were collected from Ghaemshahr in the North of Iran. The leaves were dried and the essence of the three plants was extracted. The larvaes were hatched from cysts of Artemia salina at 26˚C in filtered seawater. The plant extracts with different concentration (10,100 and 1000ppm) were added to the solution larvaes.
Results: The LC50 values (the concentration which needed to die half the larvaes) were measured for pigweed, Bermuda grass and burdock at 1640, 990, and 840 ppm, respectively.
Conclusion: The results indicate that compared to the two other species, there is more priority for burdock to be studied in further studies for identification and assessment of toxicity.
References
Saad B, Azaizeh H, Abu-Hijleh G, Said O. Safety of traditional arab herbal medicine. Evid Based CAM 2006;3(4):433-439.
Barthelson RA, Sundareshan P, Galbraith DW, Woosley RL. Development of a comprehensive detection method for medicinal and toxic plant species. Am J Bot 2006;93(4):566-574.
Palasuwan A, Soogarum S, Lertlum T, Pradniwat P, Wiwanitkit V. Inhibition of heinz body induction in an in vitro model and total antioxidant activity of medicinal Thai plants. Asian Pac J Cancer Prev 2005;6(4):458-463.
Sowemimo AA, Fakoya FA, Awopetu I, Omobuwajo OR, Adesanya SA. Toxicity and mutagenic activity of some selected Nigerian plants. J Ethnopharmacol 2007;113(3):427-432.
Tajik H, Moradi M, Razavi SM, Erfani AM, Shokouhi Sabet F. Preparation of chitosan from brine shrimp cyst shell and effects of different chemical processing sequences on the physicochemical and functional properties of the product. Molecules 2008;13(7):1263-1274.
Silva TM, Nascimento RJ, Batista MB, Agra MF, Camara CA. Brine shrimp bioassay of some species of solanum from northeastern Brazil. Revista Brasileira de Farmacogn 2007;17(21):35-38.
Caceres A, Lopez B, Gonzalez S, Berger I, Tada I, Maki J. Plants used in guatemala for the treatment of protozoal infections. I screening of activity to bacteria, fungi and American trypanosomes of 13 native plants. J Ethnopharmacol 1998;62(31):195-202.
Pisutthanan S, Plianbangchang P, Pisutthanan N, Ruanruay S, Muanrit O. Brine shrimp lethality activity of thai medicinal plants in the family meliaceae. Naresuan Univ J 2004;12(6):13-18.
Deciga-Campos M, Rivero-Cruz I, Arriaga-Alba M, Corral G, Lopez GE, Navarrete A, et al. Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. J Ethnopharmacol 2007; 110(2):334-342.
Carballo JL, Hernandez-Inda ZL, Perez P, Garcia- Gravalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnol 2002; 2(6):17.
Costello MJ, Fretwell K, Read P. Toxicity of sewage sludge to Crangon crangon and artemia salina, with reference to other marine Crustacea. Aquat Living Resour 1993;6(4):351-356.
Alanis-Garza BA, Gonzalez-Gonzalez GM, Salazar-Aranda R, Waksman de Torres N, Rivas-Galindo VM. Screening of antifungal activity of plants from the northeast of Mexico. Journal of Ethnopharmacology 2007;114(3):468-471.
Duh P. Antioxidant activity of burdock: Its scavenging effect on free-radical and active oxygen. JAOCS 1997;75(4):455-461.
Aslani M, Sifi H. Toxic plant of Iran. Mashad: Ferdosi publication;2005.p.84.[Persian].
Hlywka JJ, Beck MM, Bullerman LB. The use of the chicken embryo screening test and brine shrimp bioassays to assess the toxicity of fumonisin B1 mycotoxin. Food Chem Toxicol 1997;35(11):991-999.
Logarto Parra A, Silva Yhebra R, Guerra Sardinas I, Iglesias Buela L Comparative study of the assay of artemia salina and the estimate of the medium lethal dose in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 2001;8(5):395-400.
Hartl M, Humpf HU. Toxicity assessment of fumonisins using the brine shrimp bioassay. FCT 2000;38(44):1097-1102.
Krishnaraju AV, Rao VN, Sundararju D, Vanisree M, Tsay H, Subbaraju GV. Assessment of bioactivity of indian medicinal plants using brine shrimp lethality assay. IJASE 2005;3(4):125-134.
Kanwar AS. Brine shrimp (Artemia salina)-a marine animal for simple and rapid biological assays. CJMED 2007;2(4):236-240.
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