Isolation of Leishmania Turanica from Phlebotomus Papatasi Using ITS1 Sequencing at Zoonotic Cutaneous Leishmanianisis Focus in Damghan, Semnan Province
DOI:
https://doi.org/10.22100/jkh.v5i2-3.150Keywords:
Phlebotomus papatasi, Leishmania turanica, ITS1, Damghan.Abstract
Introduction: L.turanica is isolated from Phlebotomus Papatasi and rodents, and its pathogenetic role in humans is not clear yet.
Methods: This study aimed at determining the infection of sand flies to L.turanica in rural parts of Damghan. Sand flies were collected by sticky papers. After the identification of female specimens, their DNA was extracted and Conventional PCR technique using specific primers of ITS1 was used to search for L.turanica. Products of PCR were sequenced and analyzed with Blast software.
Results: Two hundred and eighteen specimens of female P.papatasi were examined for Leishmania infection. One out of 218 females (0.5%) was positive to L.turanica. This is the first report of natural infection of P.papatasi due L.turanica in Semnan Province and the second in Iran.
Conclusion: Pathogenesis of L.turanica is not clear on human, but, this parasite is pathogenic in laboratory mice. Furthermore, mix synchronized infection of rodents to L.major and L.turanica aggravates pathogenetic effect of L.major. Immunity of some people to Cutaneous Leishmanianisis in endemic areas can be due to the exposure to L.turanica and this can be a suitable candidate for vaccine investigations against cutaneous leishmaniasis.
References
Ardahali S, Rezaei H, Nadim A. Leishmania and Leishmaniasis. 2nd ed.Tehran: Tehran Uneversity Publication Center;1994.[Persian].
Nilforushzadeh MA, Sadeghian G. Cutaneous Leishmaniasis. 1st ed. Tehran: Oruj publication;2002.[Persian].
Yazdankhah A, Tabasi N, Mohebali M. Controlling the rural cutaneous leishmaniasis in maraveh tappeh district, golestan province. Hakim Researched Journal 1999;1:43-50.[Persian].
Hamzavi Y, Mohebali M, Edrisian GH, Foruzani A. Epidemiological study of cutaneous leishmaniasis (human infection and animal reservoir) in dashti and dashestan phlebotomus papatasi from isfahan province, iran: comparison district, booshehr province. Journal of Iranian J Publ Hlth 2000;1-4:179-190.[Persian].
Ezeddin S. Study of cutaneous leishmaniasis in rural area of Shahroud district, Semnan Province, MSc thesis. Tehran:UMS;1994.[Persian].
Abai MA, Rassi Y, Imamian H, Fateh M, Mohebali M, Rafizadeh S, et al. PCR based on identification of vectors of zoonotic cutaneous Leishmaniasis in Shahrood District, Central of Iran. Pakistan Journal of Biol Sci 2007;10(12):2061-2065.
Rassi Y, Ghasemi MM, Javadian E, Motazedian H, Rafizadeh S, Aghaei Afshar A, et al. Determine of reservoirs & vectors of cutaneous Leishmaniasis by Nested-PCR in villages of Marvdasht district, Fars province. Scientific Journal of Kerman University of Medical Sciences and Health Services 2006;14(2):134-139.[Persian].
Yaghoobi Ershdi MR, Javadian E, TahvildareBidruni GH. Leishmania major MON-26 isolated from naturally infected phlebotomus papatasi (Dip:Psychodidae) in Isfahan province, Iran. Acta Tropica 1995;59:279-82.[Persian].
Parvizi P, Ready PD. Nested PCRs and sequencing of nuclear ITS-rDNA fragments detect three Leishmania species of gerbils in sandflies from Iranian foci of zoonotic cutaneous leishmaniasis. Tropical Medicine & International Health 2008;13(9):1159-1171.
Hajaran H, Mohebali M, Alimoradi S, Abai MR, Edrissian GH H. Isolation and characterization of pathogenic Leishmania turanica from Nesokia indica (Rodentia, Muridae) by PCR-RFLP and ITS1 sequencing in Iran. Trans R Soc Trop Med Hyg 2008;990.
Strelkova MV, Pacheco RS, Bulat SA, Rakitskaya TA. Comparison of some molecular-genetic techniques for identificationof Leishmania circulating in natural foci of zoonotic cutaneousleishmaniasis in the Central Asia region. Mem Inst Oswaldo Cruz1997;92:109-14.
Guan LR, Yang YQ, Qu JQ, Shen WX. Discovery and study of Leishmania turanica for the first time in China. Bull World Health Organ 1995;73:667-72.
Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 2003;47(1):349-58.
Seyedi-Rashti MA, Nadim A. The genus phlebotomus (Dip: Psychodidae, phlebotominae) of the countries of the Eastern Mediterranean region. Iranian Journal of Public Health 1992;21:11-50.[Persian].
El Tai N0, Osman 0F, Fari M El, Presber W, Schonian G. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania cfonovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg 2000;94(5):575-79.
Kazemi-Rad E, Mohebali M, Hajaran H, Rezaei S, Mamishi S. Diagnosis and characterization of leishmania species in giemsa-stained slides by PCR-RFLP. Iranian Journal of Public Health 2008;37(1):54-60.
Parvizi P, Mauricio I, Aransay AM, Miles MA, Ready PD. First detection of Leishmania major in peridomestic Phlebotomus papatasi from Isfahan province, Iran: comparison of nested-PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast DNA. Acta Tropica 2005;93(1):75-83.
Mohebali M, Javadian E, Yaghoobi-Ershadi MR, Akhavan AA, HajjaranH, Abaei MR. Characterization of Leishmania infection in rodents from endemic areas of the I.R. of Iran. East Mediterr Health J 2004;10:591-9.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
