The Effect of Aerobic Exercise with Melatonin Consumption on the Expression of HSP60 and HSP70 Markers in Rat Myocard After Ischemic-Reperfusion
DOI:
https://doi.org/10.22100/jkh.v12i1.1680Keywords:
Apoptosis, Aerobic exercise, Melatonin, HSP60, HSP70Abstract
Introduction: The aim of study was to examine the effect of aerobic exercise with melatonin consumption on the expression of HSP60 and HSP70 markers in rat myocard after ischemic-reperfusion by isoprenaline.
Methods: 30 male Wistar rats were used weighing approximately 200-250 g with two to three months old. Then the rats divided into five groups: pilot (n=4), control (n=4), melatonin (n=4), aerobic (n=4) and melatonin aerobic (n=4). Rats in melatonin group were gavaged every day for one month using a dose of 10 mg/kg BW. Meanwhile, rats in aerobic group and melatonin aerobic group were familiar with running on treadmill for one week, and then they were exposed training course with frequency of three times weekly for one month. After one month, all rats were injected isoprenaline with dose of 150 and 125 mg/kg BW with 24 hours in two consecutive days. Finally, rats were sacrificed after confirmation of infarct and expression of HSP60 and HSP70 gene were studied by real-time method. Data were analyzed with 2-∆∆ct formula, one-way ANNOVA and Kruskal Wallis methods.
Results: The results showed that there are significant differences in HSP70 gene expression between different groups (P≤0.05) but there aren’t significant differences in HSP70 gene expression between melatonin and melatonin aerobic groups (P≥0.669). Also there aren’t significant differences in HSP60 gene expression between different groups (P≥0.05).
Conclusion: Melatonin treatment and aerobic training have negligible effect on HSP60 and HSP70 gene expression. In the other hand, aerobic exercise with consuming melatonin can decrease and increase HSP60 and HSP70 gene expression respectively.
References
Gudarzinezhad H, Khodaei M, editors, 18th ed. Self-assessment and board review. Tehran:Arjmand;2012.[Persian].
Farouni M, Arjmand M, editors. 2nd ed. Andreoli and carpenter's cecil essentials of medicine. Tehran: Arjmand;2011.[Persian].
Sepehri H, Delfi L, editors. Rastgar a culture of animal cells. Tehran:Sina Teb & Rodegon;2011.[Persian].
Le Page C, Noirez P, Courty J, Riou B, Swynghedauw B, Besse S. Exercise training improves functional post-ischemic recovery in senescent heart. Exp Gerontol 2009,44:177-82. doi:10.1016/j.exger.2008.10.003
Daryanoush F, Mohazab M, editors. Exercise cardiopulmonary function in cardiac patients. Tehran: Hatmi;2012.[Persian].
Gaeini A, Samadi A, editors. ACSM guidelines for testing and prescribed exercise. Tehran: Hatmi;2014.[Persian].
Alizadeh MK, Garakyaraghi SM, TOOsi M, Ghasemi E, Khoshavi M, Salehi M, Hassan-Zadeh A. Effect of melatonin on left ventricular function in patients with heart failure. Journal of Isfahan Medical School 2011;29:1-7
Forman K, Vara E, García C, Kireev R, Cuesta S, Acuña-Castroviejo D, et al. Beneficial effects of melatonin on cardiological alterations in a murine model of accelerated aging. J Pineal Res 2010;49:312–20. doi:10.1111/j.1600-079X.2010.00800.x
Bonior J, Jaworek J, Konturek SJ,.Pawlik WW. Increase of heat shock protein gene expression by melatonin in ar42j cells. Journal of Physiology and Pharmacology 2005;56:471.81.
Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil 2007;14:753-60. doi:10.1097/HJR.0b013e3281eacef1
Azamian Jazi A, Hafezi MR, Cheraghi J, Abdi H. The Combined Effect of Endurance Training and Atorvastatin on the Extent of Necrosis Damageand Fibrosis Tissue in Male Wistar Rats Heart after Experimental Myocardial Infarction. sjimu 2016;23:28-38.
Russel JR, Tan DX. Melatonin and cardiac pathophysiology. Heart Metab 2009;44:31-4.
Li Y, Liu H, Sun J, Tian Y, Li CH. Effect of melatonin on the peripheral T lymphocyte cell cycle and levels of reactive oxygen species in patients with premature ovarian failure. Exp Ther Med 2016;12:3589-94. doi:10.3892/etm.2016.3833
Salarilak L, Heydari R, Nejati V. The effect of melatonin in preventing changes in lipid profile and MDA production in the tissues. Iranian Journal of Endocrinology and Metabolism 2011;4:406-11.[Persian].
Hardeland R. Antioxidative protection by melatonin; multiplicity of mechanism from radical detoxification to radical avoidance. Endocrine 2005;27:119-30
Borges JP, Lessa MA. Mechanisms Involved in Exercise-Induced Cardioprotection: A Systematic Review. Arq Bras Cardiol 2015;105:71-81. doi:10.5935/abc.20150024
ShirinBayan V, Roshan D, Soleyman V. Compare the effects of different drugs on the protection of aerobic exercise induced acute cardiotoxicity antitumor. 6th National Conference of Sports Science And Physical Education Students 2011.[Persian].
Veneroso C, Tuñón MJ, González-Gallego J, Collado PS. Melatonin reduces cardiac inflammatory injury induced by acute exercise. J Pineal Res 2009;47:184–91. doi:10.1111/j.1600-079X.2009.00699.x
KUMAR KV. NAIDU M. Effect of aral melatonin on exercise-induced oxidant stress in healthy subjects. Indian Journal of Pharmacology 2002;34:256-9.
Yang B, Jain S, Ashra SY, Furness PN, Nicholson ML. Apoptosis and Caspase-3 in Long-Term Renal Ischemia/Reperfusion Injury in Rats and Divergent Effects of Immunosuppressants. Transplantation 2006;81:1442-50. doi:10.1097/01.tp.0000209412.77312.69
Shi j. He j. Lin j. Sun X. Sun F, Ou CH, et al. Distinct response of the hepatic transcriptome to Aflatoxin B1 induced hepatocellular carcinogenesis and resistance in rats. Sci Rep 2016;6:1-9. doi:10.1038/srep31898
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
