The Effect of Aerobic and Anaerobic Exercise along with Melatonin consumption on VEGFA and VEGFR2 Gene expression of rats Myocardialafter Ischemiareperfusion

Abstract

Introduction and Purpose: One of the causes of Cardiovascular Disease Is Physical inactivity and consuming oxidant foods. Therefore, the purpose of present study is evaluating the effect of aerobic and anaerobic exercise with the using melatonin on VEGFA and VEGFA2 gene expression of rats after reperfusion ischemia.
Methods:38 Wistar male rats aged between 2 to 3 month and weighing from 200 to 260 grams, were divided into seven groups including, pilot(n=14), control (n=4), Melatonin(n=4), Aerobic Exercise(n=4), anaerobic Exercise(n=4), melatonin Aerobic Exercise(n=4) and melatonin anaerobic Exercise(n=4). The pilot group was divided into two groups of ischemia and healthy for confirmation of myocardial fibrosis. Melatonin groups were gavaged  by 10 mg per kg of body weight for one month. and then Aerobic Exercise group, anaerobic Exercise, melatonin Aerobic Exercise, and melatonin anaerobic Exercise Were placed under the one-month training period such that the exercise was three times a week on the treadmill. At the end of one month, all rats were injected with isoprenaline for two consecutive days with an interval of 24 hours for induction of ischemia. Finally, the VEGFA and VEGFA2 gene expression was done using Real-time method. Results of the research were analyzed using 2^(-∆∆ct)formula, and one way variance Analysis test of ANOVA, Loon and Tuky.
Findings: Aerobic Exercise and Anaerobic Exercise treatment separately, had an increasing effect on VEGFA and VEGFR2 gene expression, separatelybut their impact was not statistically significant. Other groups, except melatonin, also increased the expression of these genes. Melatonin could reduce the expression of the VEGFA and VEGFR2 gene.
Conclusion: Long-term aerobic and anaerobic exercise, if fitted, can reduce the amount of myocardial infarction.