The Effect of Aqueous Extract of Nettle and Endurance Training on Serum CRP, Blood Glucose, Body Weight, and Changes in Pancreatic, Liver and Heart Tissues of STZ-Diabetic Rats
DOI:
https://doi.org/10.22100/jkh.v13i4.2088Keywords:
Diabetes, Inflammation, Endurance training, Extract of nettleAbstract
Introduction: The anti-diabetic effects of nettle and exercise training have been proven, but the simultaneous effects of these two types of treatment in diabetic patients had controversy. Therefore, the purpose of this study was to investigate the effect of the of nettle extract and endurance training on serum C-Reactive Protein (CRP), blood glucose, Weight and the pancreatic, liver and heart tissue changes of diabetic rats.
Methods: In an experimental study, seventy male Wistar rats (weight =227.35 ± 18.52 g) were randomly divided into seven equal groups including; 1- healthy control 2- diabetic control 3- diabetic with nettle extract 4- diabetic with metformin 5- diabetic with training 6- diabetic with training + nettle 7- diabetic with training + metformin. Diabets was induced by intraperitoneal streptozotocin (STZ) injection in diabetic and therapeutic groups other than the healthy control group. Swimming training was performed for four weeks, 5 days a week, inside an animal training pool. At the end of the treatment, the mice were anesthetized and blood samples were taken to measure glucose and CRP and also samples of pancreatic, liver and heart tissue were collected for histopathologic studies.
Results: After the treatment, glucose concentration decreased the significantly in the treatment groups compared to the diabetic control group, in which the highest reduction was seen in nettle + training and metformin group (P <0.001). Also, CRP was significantly decreased the nettle + training in compared to diabetic control group (P <0.05). Photomicrographic evaluation showed less damage and more improvement in liver, pancreas and heart tissues of treatment groups, especially the nettle + training group.
Conclusion: The results showed that the use of the nettle extract and exercise training can reduce glucose and inflammation and also can reduce the tissue damage of diabetic rats.
References
Fathi azad f gaR, maleki n, ranj doust s. Study of the hypoglycemic activity of the hydroalcoholic extract of urtica dioica in normal and diabetic ratss. Pharmaceutical Sciences. 2005(2):65-9.
Larijani B, Abolhasani F, Mohajeri-Tehrani MR, Tabtabaie O. prevalence of diabetes mellitus in Iran in 2000. ijdld 2005;4:75-83.
hasani-ranjbar SH, Larijani B. Medicinal plants as potential new target drugs in endocrine disorders- review article. Journal of Public Health 2014;43:24-34.
Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 2003;26:S5-20.
Roglic G, Unwin N. Mortality attributable to diabetes: Estimates for the year 2010. Diabetes Res Clin Pract 2010;87:15-9. doi:10.1016/j.diabres.2009.10.006
Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4-14. doi:10.1016/j.diabres.2009.10.007
Joslin EP. The Unknown diabetic postgard Med 1984;4:302-6.
Jarald E, Joshi SB, Jain DC. Diabetes VS Herbal Medicines. iums-ijpt 2008;7:97-0.
Avcı G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I. Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2006;107:418-23. doi:10.1016/j.jep.2006.03.032
Manson JE, Nathan DM, Krolewski AS, Stampfer MJ, Willett WC, Hennekens CH. A prospective study of exercise and incidence of diabetes among us male physicians. JAMA 1992;268:63-7.
DeFronzo RA, Sherwin RS, Kraemer N. Effect of Physical Training on Insulin Action in Obesity. Diabetes 1987;36:1379-85.
Hughes VA, Fiatarone MA, Fielding RA, Kahn BB, Ferrara CM, Shepherd P, et al. Exercise increases muscle GLUT-4 levels and insulin action in subjects with impaired glucose tolerance. Am J Physiol 1993;264:E855-62. doi:10.1152/ajpendo.1993.264.6.E855
Kelley DE, Goodpaster B, Wing RR, Simoneau JA. Skeletal muscle fatty acid metabolism in association with insulin resistance, obesity, and weight loss. Am J Physiol 1999;277:E1130-41. doi:10.1152/ajpendo.1999.277.6.E1130
Rogers MA, Yamamoto C, King DS, Hagberg JM, Ehsani AA, Holloszy JO. Improvement in glucose tolerance after 1 wk of exercise in patients with mild NIDDM. Diabetes Care 1988;11:613-8.
B. A. The effects of 12-wk combined aerobic/resistance training on C-reactive protein (CRP) serum and interleukin-6 (IL-6) plasma in sedentary men. YAFTE 2012;14:95-106.
Freeman DJ, Norrie J, Caslake MJ, Gaw A, Ford I, Lowe GD, et al. C-reactive protein is an independent predictor of risk for the development of diabetes in the West of Scotland Coronary Prevention Study. Diabetes 2002;51:1596-600.
Selvin E, Paynter NP, Erlinger TP. The effect of weight loss on c-reactive protein: a systematic review. Arch Intern Med 2007;167:31-9. doi:10.1001/archinte.167.1.31
Namazi N, Esfanjani AT, Heshmati J, Bahrami A. The effect of hydro alcoholic Nettle (Urtica dioica) extracts on insulin sensitivity and some inflammatory indicators in patients with type 2 diabetes: a randomized double-blind control trial. Pak J Biol Sci 2011;14:775-9.
Kavalali G, Tuncel H, Göksel S, Hatemi HH. Hypoglycemic activity of Urtica pilulifera in streptozotocin-diabetic rats. J Ethnopharmacol 2003;84:241-5.
Khori V, Golalipour MJ, Ghafari S, Gharravi AM. Chronic effect of the hydroalcholic extract of Urtica dioica leaves in regeneration of β-cells of hyperglycemic rats. Journal of Medicinal Plants 2006;1:23-30. doi:10.3923/pjbs.2006.1482.1485
Ranjbari A, Azarbayjani MA, Yusof A, Halim Mokhtar A, Akbarzadeh S, Ibrahim MY, et al. In vivo and in vitro evaluation of the effects of Urtica dioica and swimming activity on diabetic factors and pancreatic beta cells. BMC Complement Altern Med 2016;16:101. doi:10.1186/s12906-016-1064-6
Mavi A, Terzi Z, Ozgen U, Yildirim A, Coşkun M. Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biol Pharm Bull 2004;27:702-5.
Silveira RF, Curiacos de Almeida Leme JA, Correa de Almeida
Junior C, Gomes RJ, Sibuya CY, Rostom de Mello MA, Luciano E. Comparative effects of physical training and metformin in diabetic rats. The Open Clinical Chemistry Journal 2008;1:13-6.
Zinman B RN, Campaigne BN, et al. . Physical Activity/Exercise and Diabetes. Diabetes Care. 2004;S58.
Das M, Sarma BP, Rokeya B, Parial R, Nahar N, Mosihuzzaman M, et al. Antihyperglycemic and antihyperlipidemic activity of Urtica dioicaon type 2 diabetic model rats. Journal of Diabetology 2011;2:1-6.
Swanston-Flatt SK, Day C, Bailey CJ, Flatt PR. Traditional plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice 1990;33:462-4.
Kanter M, Coskun O, Budancamanak M. Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride-treated rats. World J Gastroenterol 2005;11:6684-8.
Ellnain-Wojtaszek M BW, Kowalewski Z. Zwiazki flawonoidowe w Urtica dioica L.(Flavonoid compounds in Urtica dioica L.). Herba Pol. 1986;32(3-4):131-7.
Madadi Jaberi M, Vahidian Rezazadeh M, Mogharnasi M, Karaji Bani M. The Effect of 8 Weeks of Aerobic Training and Consumption of Hydro-alcoholic Extract of Nettle on Apelin and hs-CRP plasma Levels of Overweight and Obese Women. Armaghane danesh 2016;21:846-59.
Fathi M, Khairabadi S, Ramezani F, Hejazi K. Effect of eight weeks of aerobic training and green tea supplementation on cardiovascular risk factors in inactive overweight women. Quarterly of Horizon of Medical Sciences 2016;22:283-9. doi:10.18869/acadpub.hms.22.4.283
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