The Effects of Aerobic Exercise and Ginger Extract Loaded into Chitosan Nanoparticles on Atrial Natriuretic Peptide (ANP) Gene and Cardiac Histopathology in Rat Models of Myocardial Infarction
Authors
-
Vahid Fallahzadeh
- Dept. of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
https://orcid.org/0000-0001-7381-7175
-
Farzaneh Taghian
- Dept. of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
https://orcid.org/0000-0001-9531-2952
-
Khosro Jalali Dehkordi
- Dept. of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
https://orcid.org/0000-0003-0210-5984
DOI:
https://doi.org/10.22100/jkh.v17i4.2801Abstract
Introduction: In this study, the effects of aerobic exercises and encapsulated ginger extract (GE) into chitosan nanoparticles (CNPs) were investigated on atrial natriuretic peptide (ANP) gene expression and cardiac histopathology in rat models of myocardial infarction.
Methods: The 25 male rats were divided into five groups: Isop (85 mg/kg), Isop + CNPs, Isop + GE-CNPs, Isop + AE, and Isop + GE- CNPs + AE. The rats performed AE on a rodent treadmill five days per week for six weeks. GE- CNPs were gavaged to rats at a dose of 500 mg/kg for six weeks. After the end of the treatment period, the expression of ANP genes was carried out by Real-Time PCR.
Results: Based on our findings, the highest amount of collagen deposition was in the heart tissue of patient groups and the patient group treated with chitosan nanoparticles (P<0.05). Besides, the results of real-time PCR showed that the changes in ANP gene expression were significant only in the groups treated with aerobic exercise and ginger extract (P<0.05).
Conclusion: Regular aerobic exercises with nano-drug supplements such as ginger extract encapsulated into CNPs can reduce cardiac fibrosis and regulate ANP gene expression.
References
Mathers CD, Loncar D. Projections of global mortality and burden of disease from 2002 to 2030. Plos med 2006;3:e442. doi: 10.1371/journal.pmed.0030442
Mendis S, Puska P, Norrving B, Organization WH. Global atlas on cardiovascular disease prevention and control: World Health Organization; 2011. doi: 10.1023/a:1012220908636
Dixon I, Hata T, Dhalla NS. Sarcolemmal Na (+)-K (+)-ATPase activity in congestive heart failure due to myocardial infarction. American Journal of Physiology-Cell Physiology 1992;262:C664-C71. doi: 10.1152/ajpcell.1992.262.3.C664
González-Montero J, Brito R, Gajardo AI, Rodrigo R. Myocardial reperfusion injury and oxidative stress: Therapeutic opportunities. World Journal of Cardiology 2018;10:74. doi: 10.4330/wjc.v10.i9.74
Wexler BC, Greenberg BP. Protective effects of clofibrate on isoproterenol-induced myocardial infarction in arteriosclerotic and non-arteriosclerotic rats. Atherosclerosis 1978;29:373-95. doi: 10.1016/0021-9150(73)90114-7
Li J, Cai C, Li J, Li J, Li J, Sun T, et al. Chitosan-based nanomaterials for drug delivery. Molecules 2018;23:2661. doi: 10.3390/molecules23102661
Dugasani S, Pichika MR, Nadarajah VD, Balijepalli MK, Tandra S, Korlakunta JN. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol,[8]-gingerol,[10]-gingerol and [6]-shogaol. Journal of Ethnopharmacology 2010;127:515-20. doi: 10.1016/j.jep.2009.10.004
Ippoushi K, Azuma K, Ito H, Horie H, Higashio H. [6]-Gingerol inhibits nitric oxide synthesis in activated J774. 1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions. Life Sciences 2003;73:3427-37. doi: 10.1016/j.lfs.2003.06.022
Kundu JK, Surh Y-J. Molecular basis of chemoprevention with dietary phytochemicals: redox-regulated transcription factors as relevant targets. Phytochemistry Reviews 2009;8:333-47. doi: 10.1007/s11101-009-9132-x
Sharma N, Singh D, Rani R, Sharma D, Pandey H, Agarwal V. Chapter 13 - Chitosan and Its Nanocarriers: Applications and Opportunities. In: Tripathi DK, Ahmad P, Sharma S, Chauhan DK, Dubey NK, editors. Nanomaterials in Plants, Algae and Microorganisms: Academic Press;2019.p.267-86. doi: 10.3390/catal11080902
Sharma M, Kishore K, Gupta SK, Joshi S, Arya DS. Cardioprotective potential of Ocimum sanctum in isoproterenol induced myocardial infarction in rats. Molecular and Cellular Biochemistry 2001;225:75-83. doi: 10.1023/a:1012220908636
Hoydal MA, Wisloff U, Kemi OJ, Ellingsen o. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation 2007;14:753-60. doi: 10.1097/HJR.0b013e3281eacef1
Somboonwong J, Traisaeng S, Saguanrungsirikul S. Moderate-intensity exercise training elevates serum and pancreatic zinc levels and pancreatic ZnT8 expression in streptozotocin-induced diabetic rats. Life Sciences 2015;139:46-51. doi: 10.1016/j.lfs.2015.08.008
Sabry S. Effect of chitosan nanoparticles on haloperidol drug-induced hepatotoxicity in albino rats: Light and Electron Microscopic study. Journal of Bioscience and Applied Research 2016;2:771-8. doi: 10.21608/JBAAR.2016.109754
Hambrecht R, Gielen S, Linke A, Fiehn E, Yu J, Walther C, et al. Effects of exercise training on left ventricular function and peripheral resistance in patients with chronic heart failure: a randomized trial. JAMA 2000;283:3095-101. doi: 10.1001/jama.283.23.3095
Karimi N, Roshan VD, Bayatiyani ZF. Individually and combined water-based exercise with ginger supplement, on systemic inflammation and metabolic syndrome indices, among the obese women with breast neoplasms. Iranian Journal of Cancer Prevention 2015;8. doi: 10.17795/ijcp-3856
Evrard A, Hober C, Racadot A, Lefebvre J, Vantyghem MC. [Atrial natriuretic hormone and endocrine functions]. Annales de Biologie Clinique 1999;57:149-55. doi: 10.3389/fphys.2021.691407
Amran AZ, Jantan I, Dianita R, Buang F. Protective effects of the standardized extract of Zingiber officinale on myocardium against isoproterenol-induced biochemical and histopathological alterations in rats. Pharmaceutical Biology 2015;53:1795-802. doi: 10.3109/13880209.2015.1008147
Choi JG, Kim SY, Jeong M, Oh MS. Pharmacotherapeutic potential of ginger and its compounds in age-related neurological disorders. Pharmacology & Therapeutics 2018;182:56-69. doi: 10.1016/j.pharmthera.2017.08.010
Eleiwa NZH. Protective effect of Zingiber officinale (ginger) on doxorubicin induced oxidative cardiotoxicity in rats. Marsland press/Zhengzhou University 2013;2:2924-34. doi: 10.1002/ptr.2412
Giannuzzi P, Temporelli PL, Corrà U, Tavazzi L. Antiremodeling effect of long-term exercise training in patients with stable chronic heart failure: results of the Exercise in Left Ventricular Dysfunction and Chronic Heart Failure (ELVD-CHF) Trial. Circulation 2003;108:554-9. doi: 10.1161/01.CIR.0000081780.38477.FA
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