Bilaterl Substantia Nigra Pars Compacta-Lesioned as a Model for Memory Impairment of Parkinson's Disease in Rats
DOI:
https://doi.org/10.22100/jkh.v9i4.411Keywords:
Parkinson's disease, 6-Hydroxydopamine, Substantia nigra, Antioxidative enzymes, Memory.Abstract
Introduction: Long ago, only the movement disorder of Parkinson's disease (PD) was considered by researchers, but non-motor symptoms such as cognitive and memory disorder precedes the clinical symptoms. In the present study, a 6-hydroxydopamine (6-OHDA) rat memory impairment model of PD was used to investigate in vivo production of oxidative stress and memory disorder.
Methods: 6-OHDA (6μg/2μl of saline) was bilaterally injected in the substantia nigra pars compacta (SNC). The sham-operated rats were injected with saline. Spatial memory was evaluated in morris water maze (MWM). 6 days after neurosurgery the rats were killed and hippocampal tissues were separated on an ice-cold surface. Analysis of superoxid dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activity were performed.
Results: There was no significant difference in total traveled distance between groups therefore, injured animals had no problem in movement. There was also a significant increase in the escape latency in lesioned group as compared to the sham (P<0.000). The time spent in quadrant target zone of injured rats was significantly shorter than sham rats (P<0.002). One way ANOVA analyses showed a significant reduction in SOD antioxidant activity in the hippocampus of injured rats as compared to sham (P<0.05). Moreover this reduction was not significant in GPX and CAT enzymes.
Conclusion: Bilaterl substantia nigra pars compacta-lesioned as a model in early phase of Parkinson's disease would allow us to test new neuroprotective agents and treatment strategies.
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