The Healing Effect of Salavadora Persica Plant Extract on Skin Wound: an Experimental Animal Model
DOI:
https://doi.org/10.22100/jkh.v8i4.44Keywords:
Rat, Healing, Scar, Salavadora persicaAbstract
Introduction: The aim of the study was to determine the healing effect of salavadora persica plant extract on skin wound using an experimental animal model.
Methods: A skin wound was created on the back of 80 mature male rats randomized into two groups; while the lesions of control group were covered once daily with placebo cream, those of intervention group were covered with cream based drug including extract of "Salavadora Persica" plant. Ulcers were examined regarding infiltration rate of neutrophils on the first day, macrophages and lymphocytes on the third day, lymphocytes and fibroblasts, number of new generated vessels and healing on the fifth day, collagen aggregation, wound healing and size on the fourteenth day, and finally healing and scar status twenty one days after wounding.
Results: After twenty one days, the scar mean score (± standard deviation) was 8.13±2.78 in control and 3.04±1.79 in intervention group (P<0.001). On the fourteenth day, mean score of wound healing were 0.76±0.67 and 1.65±0.48 (P=0.02) respectively and mean score of collagen aggregation and wound size (mm) were 1.6±0.5 and 2.7±0.34 (P=0.002) and 1.2±0.34 and 0.25±0.23 (P=0.001) respectively. Infiltration rates, number of new generated vessels and healing score on 5 and 21 days after wounding were not different significantly.
Conclusion: Comparing two groups showed absolutely better conditions in intervention group regarding their collagen aggregation, wound size and scar scores after 14 and 21 days. In addition, healing in intervention group was better on the fourteenth day. Salavadora Persica showed good effect on healing trend.
References
Diegelmann RF, Rothkipf LC, Cohen IK. Measurement of collagen biosynthesis during wound healing. J Surg Res 1975;19:239-243.
Doilon CJ, Dunn MG, Bender E. Collagen fiber formation inrepair tissue: development of strength and toughness. Coll Rell Res 1985;5:481-492.
Gabbiani G, Hirschel Bj, Ryan B. Granulation tissue as acontractile organ. J Exp Med 1972;135:719-734.
Graham MF, Blomquist P, Zederfeldt B. The alimentary tract. In: Cohen IK, Diegelmann RF, Lindblad WL, editors. Wound healing: biochemical and clinical aspects. Philadelphia;1993.
Grotendorst GR, Chang T, Seppa HEJ. Platelet-derived growth factor is a chemoattractant for vascular smooth muscle cells. J Cell physiol 1983;113:261.
Irvin TT. Effects of malnutrrtion and hyperalimentation on wound healing. Surg Gynecol Obstet 1978;146:33-37.
Jackson DS. Development of fibrosis, cell proliferation and collegen biosynthesis. Ann Rheum Dis 1997;36:2-4.
Kindwall EP. Hyperbaric oxygens effect on radiation necrosis. clin plast surg 1993;20:473-483.
Knighron DR, Hunt TK, Scheunstuhl H. Oxygen tension regulates expression of angiogenesis factor by macrophages. Science 1983;221:1283-1285.
Miller EJ, Gay S. Collagen structure and function. In: Cohen IK, Diegelmann RF, Lindblad WL, editors.Wound healing : Biochemical and clinical aspects. Philadelphia;1993.
Cohen SC, Gabelnick HL, Johnson RK. Effects of antineoplastic agents on wound healing in mice. Surgery 1975;78:238-244.
Mc Gregor AD, Mc Gregor AD. Fundamental technique of plastic surgery. 9th ed. Edimburgh: Churchill- Livingstone;1995.
Bigson T. Zoo grafting: a curious chapter in history of plastic surgery. Br J Plas Surger 1995;9;8:234.
Diegelmann RF, Rothkipf LC, Cohen IK. Measurement of collagen biosynthesis during wound healing. J Surg Res 1975;19:239-243.
Galati EM, Germano MP, Rossitto A,d’Aquino A, Sanogo R. Antiulcerative evaluationof the persian toothbrush tree (salvadora persica). Pahrmacutical Biology 1999;37(5):325-328.
Galati EM, Monfort MT, Forestieri AM, Miceli N, Bader A, Trovato A. Salvadora persica L.: hypolipidemic activity on experimental hypercholesterolemia in rat. Phytomedicine 1999;6(3):181-185.
Sanjay PU, Kumar GS, Jayaveera K, Kishore KD, Ashok KC, Dhanapal R. Antimicrobial, wound healing and antioxidant activities of anthocephalus cadamba. Afr J Tradit Complement Altern Med 2007;4:481-7.
Nayak BS, Raju SS, Orette FA, Chalapathi Rao AV. Effects of hibiscus rosa sinensis L. (Malvaceae) on wound healing activity: a preclinical study in a sprague Dawley rat. International Journal of Lower Extremity Wounds 2007;6:76-81.
Shetty S, Udupa S, Udupa L. Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of ocimum sanctum Linn In rats. Evid Based Complement Alternat Med 2008;5:95-101.
Mohajeri G, Masoudpour H, Heidarpour M. The effect of dressing with fresh kiwifruit on burn wound healing. J Surg 2010;02:13.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
