The Effect of Two Different Modes of Exercise Swimming and Vitamin C Supplementation on Anemia Indices in Male Wistar Rat
DOI:
https://doi.org/10.22100/jkh.v11i1.1138Keywords:
Iron status, Swimming training, Anemia indicesAbstract
Introduction: It has been shown that long term swimming exercise leads to anemia. Therefore the aim of the present study was the effect of vitamin C supplement and maximal and submaximal swimming exercise on anemia in without iron deficiency rats.
Methods: For this purpose, 60 male wistar rats (6-8 week age and 170 -190 g weight) were divided into 6 groups: 1: Control rats (Con, n=10) 2: Vitamin C supplementation (Con+C, n=10) 3: Submaximal swimming (S, n=10) 4: Submaximal swimming + Vitamin C (S+C, n=10) 5: Maximal swimming (M, n=10) and 6: Maximal swimming and Vitamin C (M+C, n=10). Swimming training lasted for 10 weeks (5 day per week) for 60 min day−1. Rats in the vitamin C-treated groups drank water containing 0.1% Vitamin C. Submaximal and maximal exercise training carried out for 1and 3 hours per session. 5 cc blood sample was take from vena cava vein for the determine serum levels of anemia indices (serum iron, Ferritin, TIBC،Hct, Hb, reticulocytes, RBC, MCV، MCH، MCHC).
Results: Statistical analysis showed that the serum iron, ferritin, TIBC and MCV were not significantly different between groups. Reticulocytes in Con+C and S+C groups decreased significantly. Also, RBC and Hct decreased significantly in S and S+C groups, but Hb increased in S group in compared with the other groups. In this regard, MCH and MCHC significantly increased in S and S+C groups.
Conclusion: 10 week submaximal swimming exercise increased Hb, MCH and MCHC but have no effect on serum iron, Ferritin and TIBC in without iron deficiency rats. On the other hand, high intensity swimming training had no effect on anemia indices. 0.1 % Vitamin C supplement and swimming training despite an insignificant increased in ferritin has not effected on iron status in without iron deficiency rats.
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