Pancreatic IGF-1 Response to Exercise and Probiotic in High-Fat Diet Rats: Implications for Glucose and Insulin Regulation
Authors
- Seyed Ramin Rasouli Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
- Mandana Gholami - Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
-
Farshad Ghazalian
- Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
https://orcid.org/0000-0002-5805-0559
-
Hossein Abednatanzi
- Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
https://orcid.org/0000-0002-9191-1672
DOI:
https://doi.org/10.22100/jkh.v20i2.3450Keywords:
Pancreas, IGF-1, Exercise training, Lactobacillus bifidus , ObesityAbstract
Introduction: The use of exercise training and antioxidant and anti-inflammatory supplements is considered a therapeutic strategy in metabolic damage due to its beneficial effects at the cellular level. Therefore, the present study aimed to investigate the response of pancreatic IGF-1 to exercise and probiotics in rats with a high-fat diet (HFD).
Methods: Forty male Wistar rats were divided into one of five groups (n=8 in each group): healthy control, high-fat diet (HFD), HFD with aerobic exercise (HFD+Exe), HFD with probiotic supplements (HFD+Prob), and HFD with both exercise and probiotic supplementation (HFD+Exe+Prob). Before the main experiment, rats in the HFD groups were fed a high-fat diet for two months. During the six-week protocol, exercise rats underwent treadmill training, while probiotic rats received daily oral doses of Lactobacillus bifidus. At the end of the study, all rats were sacrificed to measure pancreatic IGF-1 levels using RT-PCR.
Results: The expression levels of the pancreatic IGF-1 gene in the HFD group were significantly reduced compared to the control group (P<0.05). However, the training and probiotic interventions, especially their combination, significantly increased the expression of this gene in pancreatic tissue (P<0.05). In contrast, the levels of insulin and glucose increased with HFD, and the combination treatment group effectively reduced them. However, the correlation between pancreatic IGF-1 and serum insulin and glucose was not confirmed in the therapeutic interventions (P>0.05).
Conclusion: It seems that exercise and probiotic intervention, especially when combined, can significantly affect insulin-regulating factors such as IGF-1 in the pancreas.
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