بررسی تغییرات مورفولوژیکی و بیان ژنهای بلوغ سیتوپلاسمی تخمک نارس بعد از 24 ساعت کشت آزمایشگاهی در زنان نابارور: یک مطالعه کنترل تصادفی
DOI::
https://doi.org/10.22100/jkh.v18i1.3047چکیده
مقدمه: در طی درمان زنان نابارورکاندید لقاح آزمایشگاهی (IVF)، حدود 15 درصد از تخمکهای بهدست آمده، نابالغ باقی میمانند. بنابراین، یافتن یک رویکرد آزمایشگاهی کارآمد برای قابل استفاده کردن این نوع تخمکهای نابالغ در بالین، بهخصوص در زنانی که تعداد تخمک محدودی دارند، اهمیت دارد. یکی از این رویکردها، کشت تخمک نابالغ در شرایط آزمایشگاهی میباشد به گونهای که بیان ژنهای دخیل در بلوغ سیتوپلاسمی تخمک نارس، افزایش یابد.
مواد و روشها: از چهل و هشت زن نابارور کاندید لقاح آزمایشگاهی (IVF)، نود و پنج تخمک نارس اهدایی بهدست آمد و تخمکها بهطور تصادفی در دو گروه قرار گرفتند: گروه کنترل بدون کشت و گروه مداخله بهصورت کشت 24 ساعته در شرایط آزمایشگاهی. بعد از تکمیل نمونهگیری، تخمکهای هر دو گروه بهطور جداگانه فشرده و با روش کمی واکنش زنجیرهای پلیمراز (q-PCR) از نظر سطح بیان ژنهای آدنوزین تریفسفات میتوکندری 6 (Mt-ATPase 6) و پروتئین مورفوژنتیک استخوان (BMP15) مقایسه شدند.
نتايج: سطح بیان ژنهای MT-ATPase 6 و BMP15 در گروه مداخله در مقایسه با گروه کنترل بهترتیب 12/3± 867/7 و 78/0± 327/6 برابر افزایش معنیداری داشت (001/0>P). همچنین براساس تغییرات مورفولوژیکی، 54 درصد تخمکهای نارس گروه مداخله، تقسیم میوزیس را از سرگیری کرده بودند.
نتیجهگیری: افزایش بیان دو ژن دخیل در بلوغ سیتوپلاسمی MT-ATP6 و BMP15 پس از کشت 24 ساعته در شرایط آزمایشگاهی، میتواند سبب از سرگیری تقسیم میوز در تخمکهای نارس شود و این روش در زنانی که تخمک محدود دارند، میتواند مفید باشد.
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