In Vitro Hepatocyte-Like Cells Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells

Authors

  • Masoumeh Ghaderi
  • Abdolamir Allameh
  • Masoud Soleimani
  • Mahdi Forozande Moghadam

DOI:

https://doi.org/10.22100/jkh.v7i2.79

Keywords:

Umbilical cord blood, Mesenchymal stem cells, Differentiation, Hepatocyte.

Abstract

Introduction: Using stem cells for in vitro tissue regeneration is one of the most interesting fields of research. Liver regeneration is not an exceptional area. The main objective of this study was the production of hepatocyte- like cells from the umbilical cord blood mesenchymal stem cells (MSCs).

Methods: Cells were isolated from UCB, upon a written informed consent obtained from donor mothers. The stem cells immunophenotype was analyzed using flow cytometry and multi-lineage differentiation potential was evaluated by means of in vitro differentiation assays. Finally, the expression of hepatocyte-specific markers was verified using reverse transcription-polymerase chain reaction (RT-PCR).

Results: The isolated UCB-MSCs had mesodermal differentiation capacity. If UCB-MSCs are cultured in appropriate conditions, they are able to induce hepatic-associated genes, such as albumin (Alb),α-fetoprotein (AFP), cytokeratins 18, 19 (CK18, CK19) and α-1 antitrypsin (AA). Moreover, UCB- MSCs produced albumin and α-fetoprotein upon in vitro induction of hepatocyte differentiation.

Conclusion: Mesenchymal stem cells from human umbilical cord blood are capable of tissue-specific commitment along hepatogenic lineage, proposing these cells as suitable tool for cell-based applications aimed at liver regeneration.

References

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Kazemnejad S, Allameh A, Gharehbaghian A, Soleimani M, Amirizadeh N, Jazayeri M. Efficient replacing of fetal bovine serum with human platelet releasate during propagation and differentiation of human bone marrow-derived mesenchymal stem cells to functional hepatocytes-like cells. Vox Sang 2008;95:149-58.

Haynesworth SE, Baber MA, Caplan AI. Cell surface antigens on human bone marrow-derived mesenchymal stem cells are detected by monoclonal antibodies. Bone 1992;13:69-80.

Gang EJ, Hong SH, Jeong JA, Hwang SH, Kim SW, Yang IH, et al. In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells. Biochem Bioph Res Co 2004;321:102-8.

Javazon EH, Beggs K, Flake A. Mesenchymal stem cells: paradoxes of passaging. Exp Hematol 2004;32:414-25.

Park PC, Selvarajah S, Bayani J, Zielenska M, Squire JA. Stem cell enrichment approaches. Semin Cancer Biol 2007;17:257-64.

Gudoitti JE, Bregerie O, Robert A, Debey P, Brechot C, Desdoutes C. Liver cell poliplooidization: a pivotal role for binuclear hepatocytes. J Biol Chem 2003;278:19095-101.

Alison MR, Pouslom R, Otto WR, Vig P, Brittan M. Recipies for adult stem cell plasticity:fusion cuisine or readymade. J Clin Pathol 2004;57:113-20.

Khoramroudi A, Nasiri S, Amiri I. Avaluating biochemical factors of in vitro differentiated hepatocyte like cells derived from cord blood stem cells. Armaghane Danesh 1998;3:23-34.[Persian].

Raufi A, Amini A, Azadbakht M, Aboozari M, Fathi F. In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells identified by cellular uptake of Indocyanine Green. blood 2010;7:33-40.[Persian].

Wright N, Samuelson L, Walkup MH, Chandrasekaran P, Gerber DA. Enreachment of a bipotent hepatic progenitor cell from native liver tissue. Biochem Biophys Res Commun 2008;36:367-72.

Khuu DN, Najimi M, Sokal EM. Epithelial cells with hepatobiliary phenotype: Is it another stem cell candidate for healthy adult human liver. World J Gastroenterol 2007;13:1554-60.

Kinoshita T, Miyajima A. Cytokine regulation of liver development. Biochim Biophys Acta 2002;1592:303-12.

Fujino H, Hiramatsu H, Tsuchiya A, Niwa A, Noma H, Shiota M, et al. Human cord blood cd34+ cells develop into hepatocytes in the livers of nod/scid/gc nnull mice through cell fusion. FASEB J 2007;21:3499-510.

Ek M, Soderdahl T, Kuppers-Munther B, Edsbagge J, Andersson TB, Bjorquist P, et al. Expression of drug metabolizing enzymesiin hepatocyte-like cells derived from human embryonic stem cells. Biochem Pharmacol 2007;74:496-503.

Published

2012-07-03

Issue

Section

Original Article(s)

How to Cite

In Vitro Hepatocyte-Like Cells Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells. (2012). Knowledge and Health in Basic Medical Sciences, 7(2), 50-55. https://doi.org/10.22100/jkh.v7i2.79

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