Differentiation of Umbilical Cord Derived Mesenchymal Stem Cells into Neuron-Like Cells Using Activin a and Nicotinamide

Authors

  • Darioush Bijan Nejad1 1- Dept. of Anatomical Sciences, Cellular and Molecular Research Center (CMRC), School of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran.
  • Saeed Azandeh2 2- Dept. of Anatomical Sciences, Cellular and Molecular Research Center (CMRC), School of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran.
  • Sadegh Saremi3 3- Dept. of Anatomical Sciences, Cellular and Molecular Research Center (CMRC), School of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran.
  • Anneh Mohammad Gharravi4 4- Stem Cells and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.

DOI:

https://doi.org/10.22100/jkh.v11i2.1367

Keywords:

Umbilical cord, Mesenchymal stem cells, Neuron-like cells, Activin A, Nicotinamide

Abstract

Introduction: Umbilical cord derived mesenchymal stem cells (MSCs) are one of the best sources for cell-based therapies. MSCs reveal the neuronal morphology and expression of neuronal marker in differentiation conditions. The aim of the present study was to differentiate of MSCs into neuron-like cells using Activin A and nicotinamide.

Methods: Umbilical cords were cut close to the placenta  and smaller pieces (2-4 cm) of umbilical cords prepared and Wharton's jelly extracted. MSCs were isolated by an explant culture method and surface phenotype of cells analyzed by Dako flow cytometry and the FlowJo software. MSCs were then induced for osteogenic and adipogenic differentiation. Cells were exposed to neuronal differentiation condition and treated with 20 μg / ml  Activin A and 0.1% FBS for 3 days in RPMI,then with 10 mMnicotinamide, 2% B27 and 0.1% FBS for 7 days.

Results: spindle-shaped MSCs were observed after 7-10 days of explants culture. Immunophenotype of cultured MSCs were positive for mesenchymal markers such as CD105 and CD90 but negative for hematopoietic markers such as CD34 and CD45. After MSCs differentiation to the osteogenic or adipogenic lineage, lipid droplets and calcium deposition were observed. The differentiated neural-like MSCs were appeared as pyramidal nerve-like cells with neuritis.

Some of th cells. During neuronal differentiation, axon and dendritic like process  wereosbserved in some of Bipolar and mulipolarmulipolar cells. Furthermore direct synaptic like contacts were observed between cells.

Conclusion: The present study showed that Activin A and nicotinamide could induce differentiation of MSCs into neural lineage.

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Published

2016-03-15

Issue

Section

Original Article(s)

How to Cite

Differentiation of Umbilical Cord Derived Mesenchymal Stem Cells into Neuron-Like Cells Using Activin a and Nicotinamide. (2016). Knowledge and Health in Basic Medical Sciences, 11(2), Page:32-37. https://doi.org/10.22100/jkh.v11i2.1367

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