Development of LAMP Assay For Molecular Detection of Streptococcus Pyogenes
DOI:
https://doi.org/10.22100/jkh.v11i4.1512Keywords:
Streptococcus pyogenes, Loop mediated isothermal amplification assay, Specific detection.Abstract
Introduction: Streptococcus pyogenes is the leading cause of suppurative infections and the non suppurative sequela as acute rheumatic fever, acute glomerulonephritis, and reactive arthritis. Loop-mediated isothermal amplification (LAMP) assay is a rapid and sensitive method to detect bacterial infections. Therefore, we developed a LAMP assay for rapid detetion of S.pyogenes infections based on cytochrome oxidase gene(oxaA).
Methods: The LAMP assay was established using six primers targeting cytochrome oxidase gene in S.pyogenes. The performance of assay was evaluated with different concentration of MgSo4, dNTPs and primers.
Results: LAMP assay was developed with concentrations of 10 uM from F3, B3, LB ad LF primers, 40 uM of each FIP and BIP primer, 0.4mM of dNTPs and 8mM of MgSo4 at 63°C for 1h.
Conclusion: The LAMP assay to detect S.pyogenes is rapid method in comparison to PCR.
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