Aspartate Aminotransferase and Alkaline Phosphates Fluctuations Pattern During Delayed Muscle Soreness Induced by Resistance Exercise in Response to Whey Protein Supplementation in Sedentary Student Boys

Abstract

Introduction: Despite antioxidant properties of whey protein, its effect on liver enzymes after resistance exercise in un-trained individuals has remained unknown. The aim of this study was to determine the effect of whey protein supplementation on Aspartate aminotransferase (AST) and Alkaline phosphates (ALP) as liver enzymes during muscle soreness induced by resistance exercise in un-trained individuals.

Methods: In this double-blind randomized controlled trial study, twenty eight un-trained student boys (78±12 kg) were divided in glutamine (3 times/ 0.4 g per kg of body weight) and placebo groups. Blood samples were taken before, immediately after and 24, 48 and 72 h after an intense resistance exercise session consist of leg eccentric contraction and analyzed for AST and ALP activity. Analysis of Variance (repeated measure) was used for compare the data between 2 groups.

Results: Compared to pre-test, a significant increase occurred in AST and ALP immediately after exercise in 2 groups (P<0.05). Based on ANOVA results, whey protein supplementation did not resulted in significant changes in AST and ALP in recovery period (24, 48 and 72 h recovery) compared with placebo group (P>0.05).

Conclusion: Based on results, it was concluded that whey protein supplementation after resistance exercise can not affect the determinative enzymes for liver damage in un-trained subjects.