The Effect of High Intensity Interval Training on Injury Induced Ischemia-Reperfusion Myocardium in Male Wistar Rats

Abstract

Introduction: Ischemic preconditioning provides protective effects on ischemia-reperfusion Injury. Exercise training is a condition for ischemic preconditioning by attenuating of stress oxidative could decrease infarction size after ischemia reperfusion. The aim of study was to study the protective effect of high intensity interval training on stress oxidative and myocardial infarction–induced ischemia -reperfusion injury in male wistar rats.

Methods: 20 male wistar rats were randomly assigned in control (Con; n=10) and high intensity interval training (HIIT; n=10). Exercise training was done for 8 weeks, 5 days per week and lasted 30 min per session. Intense interval training consists of 10 intervals of the running with an intensity of 90% of maximal oxygen consumption (VO₂max) for 2 min and 1 min of running with 50% VO₂max. 72 hr after last training session, animals were underwent surgery, and coronary occlusion was achieved by blocking aorta; for 30 min followed by a 120-min period of reperfusion. Glutathione peroxidase (GPX), Glutathione (GSH), Catalase (CAT), Myeloperoxidase (MPO), Malondialdehyde (MDA) and Myocardial infarct size  were measured.

Results: Results showed that there was no significant difference between HIIT and Control in GPX and MPO activity following ischemia-reperfusion (P≥0.05). However, CAT activity (P=0.004) and GSH content (P=0.006) significantly were higher in HIIT than control. Also, MDA concentration was greater in HIIT than control (P=0.006). While, myocardial infarct size significantly was lesser in HIIT than control (P=0.004).

Conclusion: based on the results, it can be said that high intensity interval training can prevent myocardial infarction following ischemia-reperfusion by preconditioning via improving some antioxidant factors, and could been considered as important precautionary method in myocardial infarction events.