The Effect of 8 Weeks Continuous Endurance and High Intensity Interval Training on Cardiac Tissue foxO1 and foxO3a Expression Levels in Male Rats

Authors

  • Sadaf Kara’i 1 1- Exercise Physiology MSc, Alborz Payam Noor University, Karaj Branch, Iran. orcid http://orcid.org/0000-0003-3628-5485
  • Ali Asghar Ravasi 2 2- Dept. of Physical Education, Tehran University, Tehran, Iran.
  • Majid Gholipour 3 3- Physical Education Center, Sharif University of Technology, Tehran, Iran

DOI:

https://doi.org/10.22100/jkh.v13i2.1892

Keywords:

Continuous endurance training, High intensity interval training, Gene expression, FoxO1, FoxO3a.

Abstract

 

Introduction: The purpose of this study was to investigate the effect of continuous endurance training (CET) and high intensity interval training (HIIT) on the gene expression of foxO1 and foxO3 a in male rats’cardiac tissue.

Methods: 18 rats were studied under standard condition. CET protocol was performed 5 sessions per week at 70% VO2 max and HIIT protocolwas performed 3 sessionsper week at 90% VO2 max for two minutes in the first and second weeks and then 110% VO2 max for the rest of the training period. Low intensity intervals were performed at 40% VO2 max for two minutes since the first week to the end of the third week and 30% VO2 max for the rest of the training period. Finally, foxO1 and foxO3a gene expression level were assessed by Real time - PCR and using the formula 2-ΔΔct. The results were evaluated using Kruskal-Wallis test with a significance level of P < 0.05.

Results: The results show that compared to control group, CET caused a significant reduction in foxO1 and foxO3a gene expression (both P=0.004). HIIT caused a significant reduction in foxO3a gene expression (P=0.004) but had no significant effect on reduction of foxO1 gene expression level(P=0.055). The levels of genes expression were significantly differed in the two experimental groups (P=0.002).

Conclusion: According to the results, both training protocols of the presentstudy may lead to effective training adaptations.

Author Biographies

  • Sadaf Kara’i 1, 1- Exercise Physiology MSc, Alborz Payam Noor University, Karaj Branch, Iran.

    کارشناس ارشد فیزیولوژی ورزشی

  • Ali Asghar Ravasi 2, 2- Dept. of Physical Education, Tehran University, Tehran, Iran.
    استاد دانشگاه تهران گروه فیزیولوژی
  • Majid Gholipour 3, 3- Physical Education Center, Sharif University of Technology, Tehran, Iran
    استادیار. عضو هیئت علمی تربیت بدنی دانشگاه صنعتی شریف

References

Kelley D. heart disease: causes, prevention and current research. Jccc Honors Journal. 2014; V5.

Tao L, Bei Y, Zhang H, Xiao J, Li X. Exercise for the heart: signaling pathways. Oncotarget. 2015;6 (25): 20773-20784.

Maiese K, Chong ZZ, Shang YC, Hou J. FOXO proteins: cunning concepts and considerations for the cardiovascular system. Clin Sci(Lond). 2009; 116(3): 191-203.

Paik JH, Kollipara R, Chu G, Ji H, Xiao Y, Ding Z, et al. FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis. Cell. 2007; 128(2): 309–323.

Slopack D, Roudier E, Liu ST, Nwadozi E, Birot O, Haas T. forkhead boxO transcription factor restrain exercise-induced angiogenesis. J physiol. 2014; 592(pt18): 4069-4082.

Maiese K, Chong ZZ, Shang YC. Sly as a FOXO: new paths with Forkhead signaling in the brain. Curr Neurovasc Res.2007; 4(4): 295–302.

Jagani Z, Singh A, Khosravi-Far R. FoxO tumor suppressors and BCR-ABL-induced leukemia: a matter of evasion of apoptosis. Biochimca et Biophysica Acta. 2007; 1785(1): 63–84.

Wang Y, Zhao Y, Graves DT. FoxO transcription factors: their clinical significance and regulation. BioMed Research International.2014; Article ID 915350. Available from: http://dx.doi.org/10.1155/2014/915350.

Calnan DR, Brunet A. The foxO code. Oncogene. 2008; 27(16): 2276-2288.

Sanchez AMJ. foxO transcription factors and endurance training: a role for foxO1 and foxO3 in exercise_induced angiogenesis. J Physiol. 2015; 593(pt2): 363-364.

Liu ST. Regulation of exercise induced endothelial sprout formation. Unpublished thesis. Toronto: kinesiology and health science York Univ: 2014.

Haung CC, Wang T, Tung YT, Ling WT. Effect of exercise training on skeletal muscle SIRT1 and PGC_1alpha expression levels in rats of different age. Int J Med Sci. 2016; 13(4): 260-270.

Li M, Li W, Yoon J, Jeon B, Lee SK. Resistance exercise training increase activation of AKT-eNOS and ReF-1 expression by foxO-1 activation in aorta of F344 rats. J Exerc Nutr Biochem. 2015; 19(3): 165-171.

Marfe G, Manzi V, Tafani M, Pucci B, Gambacurta A, Russo MA, et al. the modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. Journal of Molecular and Integrative Physiology.2012: 65-74. Available from: doi:10.4236/ojmip. 2012. 23010.

Kavasiz AN, Smuder AJ, Powers SK. effects of short_term endurance exercise training on acute doxorubicin_induced foxO transcription in cardiac and skeletal muscle. J Appl Physiol. 2014; 117(3): 223-230.

Williamson DL, Raue U, Slivka DR, Trappe S. resistance exercise, skeletal muscle foxO3a, and 85 year_old women. J Gerontol A Biol Sci. 2010; 65A (4): 335-343.

Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. maximum oxygen consumption of rats and its changes with various experimental procedures. J Appl Physiol Respir Environ Exerc Physiol. 1979; 47(6): 1278-1283.

Tharrington IH. skeletal muscle forkhead Box3A (foxO3a) response to acute resistance exercise in young and old men and women: relationship to muscle glycogen content and 5_AMP activated protein kinase(AMPK) activity. Unpublished thesis. East Carolina Univ: 2010.

Tucker PS, Briskey DR, Scanlan AT, Coombes JS, Dalbo VJ. High intensity interval training favourably affects antioxidant and inflammation mRNA expression in early-stage chronic kidney disease. Free Radic Biol Med. 2015; 89: 466-472.

Frazzi R, Valli R, Tamagnini I, Casali B, Latruffe N, Merli F. Resveratrol-mediated apoptosis of Hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation. Int J Cancer.2013; 132(5): 1013–1021.

Li T, Zhang J, Feng J, Li Q, Wu L, Ye Q, et al. Resveratrol reduces acute lung injury in a LPS? Induced sepsis mouse model via activation of Sirt1. Mol Med Rep. 2013; 7(6): 1889–1895.

Oellerich MF, Potente M. FOXOs and sirtuins in vascular growth, maintenance, and aging. Circ Res. 2012; 110(9): 1238–1251.

Zarzuelo MJ, López-Sepúlveda R, Sánchez M, Romero M, Gómez-Guzmán M, Ungvary Z, et al. SIRT1 inhibits NADPH oxidase activation and protects endothelial function in the rat aorta: implications for vascular aging. Biochem Pharmacol. 2013; 85(9): 1288–1296.

Ferrer MD, Tauler P, Sureda A, Tur JA, Pons A. Antioxidant regulatory mechanisms in neutrophils and lymphocytes after intense exercise. J Sports Sci. 2009; 27(1):49–58.

Arany Z, He H, Lin J, Hoyer K, Handschin C, Toka O, et al. Transcriptional coactivator PGC-1 alpha controls the energy state and contractile function of cardiac muscle. Cell Metab. 2005; 1(4): 259 –271.

Lehman JJ, Barger PM, Kovacs A, Saffitz JE, Medeiros DM, Kelly DP. Peroxisome proliferator-activated receptor gamma coactivator-1 promotes cardiac mitochondrial biogenesis. J Clin Invest. 2000; 106(7): 847–856.

St-Pierre J, Lin J, Krauss S, Tarr PT, Yang R, Newgard CB, et al. Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells. J Biol Chem. 2003; 278(29): 26597–26603.

Hudlicka O, Brown MD, Egginton S. Angiogenesis in skeletal and cardiac muscle. Physiol Rev.1992;72(2): 369-417.

Bloor CM. Angiogenesis during exercise and training. Angiogenesis. 2005; 8(3): 263–271.

Gustafsson T. Vascular remodeling in human skeletal muscle. Biochem Soc Trans. 2011; 39(6): 1628–1632.

Published

2018-09-12

Issue

Section

Original Article(s)

How to Cite

The Effect of 8 Weeks Continuous Endurance and High Intensity Interval Training on Cardiac Tissue foxO1 and foxO3a Expression Levels in Male Rats. (2018). Knowledge and Health in Basic Medical Sciences, 13(2), 62-70. https://doi.org/10.22100/jkh.v13i2.1892

Most read articles by the same author(s)

<< < 2 3 4 5 6 7 8 9 10 11 > >>