The ratios of human arginase activity to other biochemical parameters in the serum of pre- and post-hemodialysis patients as new clinical indices

Authors

  • Nematollah Eazmi استاديار دانشكده دامپزشكي دانشگاه شيراز
  • Saeid Nazifi استاد دانشكده دامپزشكي دانشگاه شيراز
  • Hamid Derazhi دانش آموخته دانشكده دامپزشكي دانشگاه شيراز

DOI:

https://doi.org/10.22100/jkh.v2i2.228

Keywords:

Arginase, Hemodialysis patients, renal failure, Dialysis.

Abstract

Introduction: Prognosticators are indices which are used by medical specialists in early detection to make better decisions about treatment in hemodialysis patients. In this study the ratios of human arginase activity with other biochemical parameters in serum of pre- and post- dialysis patients were compared to healthy individuals. The ratios evaluated as new indices for diagnosis and treatment of the patients. 

 

Methods and Materials: In this study, 286 human serum specimens were evaluated, 206 of which belonged to the hemodialysis patients (103 of pre- and 103 of post- dialysis) and 80 to the healthy individuals. Arginase activity were determined by urea method, blood urea nitrogen by urease method, uric acid by modified Folin method, creatinine by Jaffe method and total protein was measured by biuret method.

 

Results: The results showed that the serum arginase activity of hemodialysis patients (pre- and post- dialysis) was significantly higher than healthy subjects (p < 0.01). It might be concluded that hemodialysis of patients had no effect on their serum arginase.  There were significant differences between   ratio of serum arginase activity and blood urea nitrogen, creatinine and uric acid in hemodialysis patients (pre- and post- dialysis) with healthy individuals as well as  pre- and post- hemolysis patients (p<0.01). There were no significant differences between arginase specific activity of the three groups (p>0.01).

 

Conclusion: This investigation suggests that in parallel with other serum biochemical tests, it is beneficial to measure arginase activity and using the ratios of arginase activity to other biochemical serum parameters as new indices in prognosis and treatment of hemodialysis patients.

References

Isselbacher KJ, Braunwald E, editors. Harrison’s principles of internal medicine. 13th ed. New York: McGraw -Hill, Inc; 1994.

Bach JS, Killip M. Purification and crystalization of arginase. Biochem Biophys Acta 1958; 29: 273- 280.

Meyer DJ, Harvey JW, editors. Veterinary laborat¬- ory medicine: interpretation and diagnosis. 2nd ed. WB Saunders Co; 1998.

Kaneko JJ, editors. Clinical biochemistry of domes¬¬tic animals. 4th ed. New York :Academic Press Inc; 1989.

Duncan JR, Prasse KW, editors. Veterinary labora- tory medicine clinical pathology. 3rd ed. Iowa State University Press: Ames Iowa; 1994.

Folin O. An improved method for the determination of uric acid in blood. J Biol Chem 1930; 86: 179- 187.

Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: WB Saund- ers Co; 1994.

Gopalakrishna R, Nagarajan B. A simplified procedure for the estimation of arginase in plasma and urine using arginine. Clin Chemic Acta 1980; 106: 333– 337.

Natelson S, editor. Techniques of clinical chemistry. 3rd ed. USA: Thomas Publisher; 1971.

Cornelius CE, Freedland RA. The determination of arginase activity in serum by mean’s of gel filtration. Cornell Vet 1961; 52: 344– 360.

Baranczyk K, Iwonaskrzypek O. Arginase from human blood serum. Biochemi Med 1981; 26: 174– 184.

Bishop ML, Duben- Engelkirk JL, editors. Clinical chemistry. Principle, procedures, correlations. 3rd ed. Philadelphia: Lippincott; 1996.

Rac RM, Mishra OP. Dermal wound healing in experimentally injured rats: Role of arginase. Nutr Rep Int 1980; 22: 167– 172.

Grody W. Differential expression of the two human arginase gene in hyperargininemia. J Clin Invest 1989; 83: 602– 609.

Pelkin V, Kalub M. Determination of arginase activity in blood in epidemic hepatitis. Clin Chem Acta 1984; 9: 141– 147.

Lehninger AL, Nelson DL, editors. Principles of biochemistry. 2nd ed. New York: Worth Publishers; 1993.

Maning RT, Grisolia A. Serum arginase activity. Exp Biol Med 1954; 95: 225– 235.

Published

2007-10-02

Issue

Section

Original Article(s)

How to Cite

The ratios of human arginase activity to other biochemical parameters in the serum of pre- and post-hemodialysis patients as new clinical indices. (2007). Knowledge and Health in Basic Medical Sciences, 2(2), 2-5. https://doi.org/10.22100/jkh.v2i2.228

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