Sequencing and Drawing the Phylogenic Tree of Endophytic Bacteria Collected from Rhabdosciadium Aucheri by the Sanger Sequencing and Determining their Antifungal Effect
Authors
-
Naqisa Zarei
- Department of Microbiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamadan, Iran.
https://orcid.org/0009-0004-3562-7513
-
Reza Habibipour
- Assistant Professor, Department of Microbiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
https://orcid.org/0000-0002-1632-2624
DOI:
https://doi.org/10.22100/jkh.v20i2.3427Keywords:
Aspergillus, Bacillus, Sequencing, Endophytic bacteria,Abstract
Introduction: The unique properties of endophytic bacteria are not well known. Endophytic bacteria of some plants, such as Rhabdosciadium aucheri, may inhibit pathogenic fungi with antimicrobial effects. The purpose of this study is to sequence and draw the phylogenetic tree of R.aucheri endophytic bacteria by the Sanger sequence method and determine their antifungal effect.
Methods: In this descriptive-analytical study, plant samples were collected using a random, convenient, and accessible method. The initial identification of endophytic bacteria was performed using the plate culture technique. Molecular detection and sequencing of the bacteria were carried out using optimized PCR methods.
Results: Out of 100 bacterial isolates obtained from R. aucheri, the distribution was as follows: Bacillus pumilus made up 9% (9 isolates), Bacillus subtilis 15% (15 isolates), Serratia ficaria 12% (12 isolates), Bacillus amyloliquefaciens 14% (14 isolates), Pantoea agglomerans 8% (8 isolates), and Pseudarthrobacter oxydans 7% (7 isolates). Sequencing analysis indicated that variations among the Bacillus species were primarily associated with differences at the cytosine (C) and guanine (G) nucleotide positions. Among the isolates, those belonging to the Bacillus genus displayed the most significant inhibitory effect against Aspergillus niger.
Conclusion: Our findings indicate that the roots of the R. aucheri plant provide a favorable environment for isolating Bacillus species with high antimicrobial activity. Among the isolated bacteria, Bacillus subtilis exhibited the most potent antifungal effect against pathogenic fungi.
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