The Effect of Interval Training on Tumor Volume in Mice with Breast Cancer

Authors

  • Omid Salehian
  • Rahman Souri
  • Zoheir Mohammadhasan
  • Aliasghar Ravasi

DOI:

https://doi.org/10.22100/jkh.v8i4.42

Keywords:

Interval training, Tumor mass, Hsp70, IL4, IFNγ.

Abstract

Introduction: Exercise has effects on changing cancer factors, yet the effect of exercise on the levels of cancer factors in inflicted animals or humans has not been seriously paid attention to. Thus the aim of this study is to study the effect of aerobic continuous training on tumor volume on mice with carcinoma.

Methods: In this study, 30 female balb/c mice were selected and after transplanting adenocarcinoma tumor in peritoneal area, the mice were randomly divided into a tumor-control and interval training-tumor groups. Interval training protocol was done for 7 weeks at 20% vo2max in the first week and 75% vo2max in the last week. Speleenectomy where doneafter interval training protocol, and Eliza method used to measure Heat Shock Protein 70 (HSP70), Interleukin 4 (IL4) and Interferon γ (IFNγ).

 Results: The results of this study showed a decrease in the amount of Hsp70 in interval training group but the difference between the two groups was not significant (P>0.05). The results also showed a decrease in the levels of IL4 in the interval training-tumor group but there was no significant difference between the groups (P>0.05). The levels of IFN in interval training-tumor group were higher than those of the control group but this difference was not significant either (P>0.05). However, tumor mass in interval training-tumor group significantly decreased (P=0.001).

Conclusion: Based on the results of this research, it can be concluded that doing interval training through changing the amount of effective factors on cancer can lead to a decrease in volume mass.

References

Holick CN, Newcomb PA, Trentham-Dietz A, Titus-Ernstoff L, Bersch AJ, Stampfer MJ, et al. Physical activity and survival after diagnosis of invasive breast cancer. Cancer Epidemiol Biomarkers Prev 2008;17(2):379-86.

Garaude J, Kent A, Van Rooijen N, Blander JM. Simultaneous targeting of toll- and nod-like receptors induces effective tumor-specific immune responses. Sci Transl Med 2012;4(120):120ra16.

Georgopoulos C, Welch WJ. Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol 1993;9:601-34.

Hightower LE, Hendershot LM. Molecular chaperones and the heat shock response at cold spring harbor. Cell Stress Chaperones 1997;2(1):1-11.

Kregel KC. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol 2002;92(5):2177-86.

Rohde M, Daugaard M, Jensen MH, Helin K, Nylandsted J, Jaattela M. Members of the heat-shock protein 70 family promote cancer cell growth by distinct mechanisms. Genes Dev 2005;19(5):570-82.

Daugaard M, Jaattela M, Rohde M. Hsp70-2 is required for tumor cell growth and survival. Cell Cycle 2005;4(7):877-80.

Zielinski MR, Muenchow M, Wallig MA, Horn PL, Woods JA. Exercise delays allogeneic tumor growth and reduces intratumoral inflammation and vascularization. J Appl Physiol 2004;96(6): 2249-56.

Banfi G, Dolci A, Verna R, Corsi MM. Exercise raises serum heat-shock protein 70 (Hsp70) levels. Clin Chem Lab Med 2004;42(12):1445-6.

Samelman TR. Heat shock protein expression is increased in cardiac and skeletal muscles of Fischer 344 rats after endurance training. Exp Physiol 2000;85(1):92-102.

Ogata T, Oishi Y, Higashida K, Higuchi M, Muraoka I. Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. Am J Physiol Regul Integr Comp Physiol 2009;296(5):R1557-63.

Ru W, Peijie C. Modulation of NKT cells and Th1/Th2 imbalance after alpha-GalCer treatment in progressive load-trained rats. Int J Biol Sci 2009;5(4):338-43.

Mastro AM, Schlosser DA, Grove DS, Lincoski C, Pishak SA, Gordon S, et al. Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise. Med Sci Sports Exerc 1999;31(1):74-81.

Shimizu K, Kimura F, Akimoto T, Akama T, Tanabe K, Nishijima T, et al. Effect of moderate exercise training on T-helper cell subpopulations in elderly people. Exerc Immunol Rev 2008;14:24-37.

Ernest D, Olfert DV MB, Cross M , Ann A. Guide to the care and use of experimental animals-edited. McWilliams. 1993

Feyzi R, Hassan ZM, Mostafaie A. Modulation of CD(4)(+) and CD(8)(+) tumor infiltrating lymphocytes by a fraction isolated from shark cartilage: shark cartilage modulates anti-tumor immunity. Int Immunopharmacol 2003;3(7):921-6.

Noori S, Hassan ZM. Tehranolide inhibits proliferation of MCF-7 human breast cancer cells by inducing G0/G1 arrest and apoptosis. Free Radic Biol Med 2012;52(9):1987-99.

Locke M, Noble EG, Atkinson BG. Exercising mammals synthesize stress proteins. American Journal of Physiology - Cell Physiology 1990;258(4):C723-C9.

Niess AM, Simon P. Response and adaptation of skeletal muscle to exercise--the role of reactive oxygen species. Front Biosci 2007;12:4826-38.

Milne KJ, Wolff S, Noble EG. Myocardial accumulation and localization of the inducible 70-kDa heat shock protein, Hsp70, following exercise. J Appl Physiol 2012;113(6):853-60.

Khassaf M, Child RB, McArdle A, Brodie DA, Esanu C, Jackson MJ. Time course of responses of human skeletal muscle to oxidative stress induced by nondamaging exercise. J Appl Physiol 2001;90(3):1031-5.

Fehrenbach E, Niess AM, Voelker K, Northoff H, Mooren FC. Exercise intensity and duration affect blood soluble HSP72. Int J Sports Med 2005;26(7):552-7.

Suzuki K, Nakaji S, Yamada M, Totsuka M, Sato K, Sugawara K. Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc Immunol Rev 2002;8:6-48.

Giraldo E, Garcia JJ, Hinchado MD, Ortega E. Exercise intensity-dependent changes in the inflammatory response in sedentary women: role of neuroendocrine parameters in the neutrophil phagocytic process and the pro-/anti-inflammatory cytokine balance. Neuroimmunomodulation 2009;16(4):237-44.

Nicolini A, Carpi A. Immune manipulation of advanced breast cancer: an interpretative model of the relationship between immune system and tumor cell biology. Med Res Rev 2009;29(3):436-71.

Parker DC. T cell-dependent B cell activation. Annu Rev Immunol 1993;11:331-60.

Kacha AK, Fallarino F, Markiewicz MA, Gajewski TF. Cutting edge: spontaneous rejection of poorly immunogenic P1.HTR tumors by Stat6-deficient mice. J Immunol 2000;165(11):6024-8.

Agarraberes FA, Terlecky SR, Dice JF. An intralysosomal hsp70 is required for a selective pathway of lysosomal protein degradation. J Cell Biol 1997;137(4):825-34.

Pockley AG. Heat shock proteins as regulators of the immune response. Lancet 2003;362(9382):469-76.

Barlow C, Liyanage M, Moens PB, Deng CX, Ried T, Wynshaw-Boris A. Partial rescue of the prophase i defects of Atm-deficient mice by p53 and p21 null alleles. Nat Genet 1997;17(4):462-6.

Verma VK, Singh V, Singh MP, Singh SM. Effect of physical exercise on tumor growth regulating factors of tumor microenvironment: implications in exercise-dependent tumor growth retardation. Immunopharmacol Immunotoxicol 2009;31(2):274-82.

Jones LW, Eves ND, Courneya KS, Chiu BK, Baracos VE, Hanson J, et al. Effects of exercise training on antitumor efficacy of doxorubicin in MDA-MB-231 breast cancer xenografts. Clin Cancer Res 2005;11(18):6695-8.

Published

2013-07-02

Issue

Section

Original Article(s)

How to Cite

The Effect of Interval Training on Tumor Volume in Mice with Breast Cancer. (2013). Knowledge and Health in Basic Medical Sciences, 8(4), Page:144-149. https://doi.org/10.22100/jkh.v8i4.42

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