Role of Progesterone Administration on Endometrial Angiogenesis and other Endometrial Quantative Changes in Hyperstimulated Mice at Implantation Period

Authors

  • Sedigheh Momeni1 Dept. of Biology, School of Sciences, Arak University, Arak, Iran
  • Mandana Beigi Boroujeni2 Dept. of Anatomy, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Hamid Reza Momeni3 Dept. of Biology, School of Sciences, Arak University, Arak, Iran.
  • Nasim Beigi Boroujeni4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Ezatollah Rafiee Alavi5 Dept. of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Mohamad Reza Gholami6 Dept. of Anatomy, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

DOI:

https://doi.org/10.22100/jkh.v10i4.848

Keywords:

آندومتر، پروژسترون، تعداد مقطع عروق،تغییرات کمی اندومتر،زمان لانه گزینی

Abstract

Introduction: The vascular parameters changes from the morphological aspects resulted in changes in the rate of the embryo implantation. This event depend on theovarian hormones. In this study, the quantiative changes of the endometrium was assessed after ovarian stimulation followed by progesterone (p4) treatment.

Methods: Adult female mice were used in this study. Animals were divided in three groups. pseudopregnancy were induced in the control group. Then in the experimental group1 (ovarian induction group)and experimental group2(p4 treated after ovarian induction), ovarian stimulation was done. P4 treatment was done daily in the experimental group2 after pseudopregnancy. Finally, blood and tissue samples were taken for assessment of the ovarian hormone levels and histogical studies, respectively. Then statistical analysis was done.

Results: The results indicated the significant increase in the progesterone level, vascular sections, internal area of vessels, and the diagonal of the glandular lumen in two experimental groups (1&2) compared to the control group (P≤0.05). Also, P4 treatment after ovarian induction resulted in the significant increase in the P4 level and vascular section number in the experimental group 2 compared to the experimental group1 (P≤0.05).

Conclusion: P4 treatment after ovarian induction improve the morphological condition of the endomertium compared to the ovarian induction group. Therefore, it may result in an increased rate of successful implantation.

Author Biographies

  • Sedigheh Momeni1, Dept. of Biology, School of Sciences, Arak University, Arak, Iran

    فوق لیسانس فیزیولوژی جانوری گروه زیست شناسی

  • Mandana Beigi Boroujeni2, Dept. of Anatomy, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

    استادیار گروه اناتومی

  • Hamid Reza Momeni3, Dept. of Biology, School of Sciences, Arak University, Arak, Iran.

    گروه زیست شناسی. استادیار

  • Nasim Beigi Boroujeni4, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    استادیار دانشگاه علوم پزشکی لرستن
  • Ezatollah Rafiee Alavi5, Dept. of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
    استادیار گروه پاتولوژی
  • Mohamad Reza Gholami6, Dept. of Anatomy, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

    استادیار گروه اناتومی

References

Garris D.R. Scanning electron microscopic and morphometric analysis of the gunie pig uterine luminal surface: cyclic and ovarian steroid induced. Anat. Rec 1998; 252:205-214.

Niknafs B, Afshari F, Dezfulian A. Morphological and morphometrical changes of endometrium after application of estrogen and progesterone during luteal phase in the superovulated mice. 2008;6:133-142.

Hubayter ZR, Muasher SJ. Luteal supplementation in vitro fertilization: more questions than answers. Fertility Sterility 2008;89:749-758.

Gunian A. Effect of ovarian hormone on cell membrane in the rat uterus. Eur. J. Obset, Gynecol. Reprod Biol 1995; 60:69-74.

Beigi M, Salehnia M, Al-Tiraihi T. Morphological change in mouse endometrium after ovarian hyperstimulation during implantation period. Medical J of Yazd univ 2003;11:90-96.

Matsumoto H, Fukui E, Yoshizawa M. Uterine angiogenesis during implantation in mice, Archive bioone repository. http://www.bioone.org/perlserv 2007.

Perrot-Applanat M, Ancelin M, Buteau –lozano H, Meduri G, Bausero P. Ovarian steroids in endometrial angiogenesis. 2000;65:599-603.

Okada H, Tsuzuki T, Shindoh H, Nishigaki A, Yasuda K, Kanzaki H. Regulation of decidualization and angiogenesis in the human endometrium: Mini review. 2014;40:1180-1187.

Walter L.M, Rogers P.A.W, Girling J.E. The role of progesterone in endometrial angiogenesis in pregnant and ovariectomised mice.Reproduction 2005;129:765-777.

Hewitt SC, Korach KS. Progesterone action and responses in the ⍺ERKO mouse. 2000;65:551-557.

Spencer T.E, Bazer F.W. Biology of progesterone action during pregnancy recognition and maintenance of pregnancy. 2002;7:d1879-98.

Oral M، Conneely M, Biserka Mu, Francesco De, John P, Lydon BW, et al. Reproduction function of progesterone receptor. Recent Progress in Hormone Research 2002;57:339-335.

Takeshi Ku, Ki-jun Le, Philippa TK, Paul SC, Julia AT, Dennis B. Regulation of progesterone receptors and decidualization in uterine stroma of the estrogen receptor-knockout mouse. Biology of Reproduction 2001;64:272-283.

Wen-ge Ma, Haengseok So, Sanjoy KD, Bibhash CP, Sudhansu KD. Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. PNAS 2003;100:2963-2968.

Losordo D.W, Isner J.M , Estrogen and Angiogenesis: A Review, Journal of the American Heart Association 2001; 21:6-12.

Kolb B.A, Najmabadi S, Paulson R.J, Ultrastructural characteristics of the luteal phase endometrium in patients undergoing controlled ovarian hyperstimulation, Fertil Steril.1997;67(4):625-30.

Salehnia M, Rasi Ghaemi S, Reza Zadeh M. Hanges in implantation and survival rates and embryo quality after progesterone. Anatomical Sciences Journal 2005;3:93-100.

Beigi M, Salehnia M, Al-Tiraihi T. Morphological change in mouse endometrium after ovarian hyperstimulation during implantation period, Medical J of Yazd univ. 2003;11(4):90-96.

Arianmanesh M, Salehnia M,Niknafs B. effect of progesterone on the ulterastructure of endometrium uterus after hyperstimulation mice at pre-implantation period. Yakhteh 2003;61-65.

Salehnia M. Different Pattern of Expression in stimulated Mouse Endometrium. Exp.Anim 2005;54:349-352.

Rafighdoost H, Tavafi M, Jafari pour L, Rasoulian B, Ahmadvand H, Mahmodi M, et al. Effect of olive leaf extract in inhibition of ranal ischemia reperfusion injuries in rat. Anatomical sciences 2013;10:160-165.

Dalagaard M, Pilegaard K, Ladefoged O. In utero exposure to diethylstilbestrol or 4-n-nonylphenol in rats: number of sertoli cells, diameter and length of seminiferous tubules estimated by stereologicalmethods. Pharmacol Toxicol 2002;90:59-65.

Hervé M.A.J, Meduri G, Petit F.G, Domet T.S, Lazennec G, Mourah S, Applanat M.P. Regulation of the vascular endothelial growth factor (VEGF) receptor Flk-1/KDR by estradiol through VEGF in uterus. 2006;188:91-99.

Ma W, Tan J, Matsumoto H, Robert B, Abrahamson DR, Das SK, Dey SK. Adult tissue angiogenesis: evidence for negative regulation by estrogen in the uterus. Molecular Endocrinology 2001;15:1983–1992.

Kayisli UA, Luk J, Guzeloglu-Kayisli O, Seval Y, Demir R, Arici A. Regulation of angiogenic activity of human endometrial endothelial cells in culture by ovarian steroids. Journal of Clinical Endocrinology and Metabolism 2004; 89:5794–5802.

Rogers P.A.W, Donoghue J.F, Walter L.M, Girling J.E. Endometrial Angiogenesis, Vascular Maturation, and Lymphangiogenesis.Reproductive scinces 2009;16:147-51.

Salehnia M, Arianmanesh M, Beigi M. The impact of ovarian stimulation on mouse endometrium: a morphometrial study. 2006;4:7-11.

Klentzeris L.O. The role of endometrium in implantation. Hum. Repord, 12 Natl (supp) JBFS 1997;2:170-175.

Standring S. Grayˊs Anatomy: Female Reproduction system (implantation, placentation pregnancy and parturition). 39 Edinbourg: Elsevier Churchill Livingstone. Press:2005.P.1349.

Bailey D.W, Dunlap K.A, Frank J.W, Erikson D.W, White B.G, Bazer F.W, et al. Effect of long-term progesterone on developmental and functional aspects of porcine uterine epithelial and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy. 2010;140:583-94.

Deligdisch L. Hormonal pathology of the endometrium. Mod-Pathol 2000; 13:258-94.

Published

2015-09-28

Issue

Section

Original Article(s)

How to Cite

Role of Progesterone Administration on Endometrial Angiogenesis and other Endometrial Quantative Changes in Hyperstimulated Mice at Implantation Period. (2015). Knowledge and Health in Basic Medical Sciences, 10(4), Page:31-39. https://doi.org/10.22100/jkh.v10i4.848

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 9 10 > >>