The Effect of Progesterone Administration on the Number of Secondary Follicles of Ovulated Mice During Pre-Implantation

Authors

DOI:

https://doi.org/10.22100/jkh.v14i1.2193

Keywords:

Secondary Follicle, Ovarian Stimulation, Progesterone, Pre-Implantation

Abstract

Introduction: Ovulation induction using gonadotropins increases the progesterone at the end of follicular phase and subsequently has a negative effect on the outcome of ovulation induction. The aim of this study was evaluation of the effect of progesterone administration on the number of secondary follicles on ovulation

Methods: In this study, 15 adult female rats were randomly divided into three groups: control, ovulation induction group and ovulation induction group with progesterone administration. In experimental groups, ovulation stimulation was performed with hMG and hCG, and then false pregnancy was induced in all three groups. In Experimental group 2, after induction of pregnancy, 1 mg progesterone daily was injected. For histological examination, ovarian samples were taken from all three groups at the time of pre-implantation and statistical analysis perforemd.

Results: The number of secondary follicles in experimental groups 1 was significantly higher than the experimental group 2 and control group (P<0.05). However, there was no significant difference in the experimental group 2 compared to the control group.

Conclusion: Progesterone administration after ovulation stimulation inhibits folliculogenesis and thus prevents excessive ovarian stimulation syndrome. Progesterone administration balance ovulation stimulation.

References

Santos MA, Kuijk EW, Macklon NS. The impact of ovarian stimulation for IVF on the developing embryo. Reproduction 2010;139:23-34. doi: 10.1530/REP-09-0187

Bosch E, Labarta E, Crespo J, Simon C, Remohi J, Jenkins J, et al. Circulating progesterone levels and ongoing pregnancy rates in controlled ovarian stimulation cycles for in vitro fertilization: analysis of over 4000 cycles. Human Reprod 2010;25:2092-100. doi: 10.1093/humrep/deq125

Al-Azemi M, Kyrou D, Kolibianakis E, Humaidan P, Van Vaerenbergh I, Devroey P, et al. Elevated progesterone during ovarian stimulation for IVF. Reprod Biomed Online 2012;24:381-8. doi: 10.1016/j.rbmo.2012.01.010

Lawrenz B, Fatemi H. Effect of progesterone elevation in follicular phase of IVF-cycles on the endometrial receptivity. Reprod Biomed Online 2017;34:422-8. doi: 10.1016/j.rbmo.2017.01.011

Verdin H. Ovarian hyperstimulation syndrome. Head of Regulatory Policy 2018.

Aboulghar M. Prediction of ovarian hyperstimulation syndrome (OHSS) Estradiol level has an important role in the prediction of OHSS. Hum Reprod 2003;18:1140-1. doi: 10.1093/humrep/deg208

Papanikolaou EG, Tournaye H, Verpoest W, Camus M, Vernaeve V, Van Steirteghem A, et al. Early and late ovarian hyperstimulation syndrome: early pregnancy outcome and profile. Hum Reprod 2005;20:636-41. doi: 10.1093/humrep/deh638

Reynolds LP, Grazul-Bilska AT, Redmer DA. Angiogenesis in the female reproductive organs: pathological implications. Int J Exp Pathol 2002;83:151-63. doi: 10.1046/j.1365-2613.2002.00277.x

Devroey P, Bourgain C, Macklon NS, Fauser BC. Reproductive biology and IVF: ovarian stimulation and endometrial receptivity. Trends Endocrinol Metab 2004;15:84-90. doi: 10.1016/j.tem.2004.01.009

Beigi M, Salehnia M, Al-Tiraihi T. Morphological change in mouse endometrium after ovarian hyperstimulation during implantation period. Medical J of Yazd Univ 2003;11:90-6.[Persian].

Arianmanesh M, Salehnia M, Niknafs B. Effect of progesterone on the ulterastructure of endometrium uterus after hyperstimulation mice at pre-implantation period. Yakhteh 2003;61-5.[Persian].

Salehnia M. Different pattern of expression in stimulated mouse endometrium. Exp Anim 2005;54:349-52. doi: 10.1538/expanim.54.349

Momeni S, Boroujeni MB, Momeni HR, Boroujeni NB, Alavi ER, Gholami MR. Role of progesterone administration on endometrial angiogenesis and other endometrial quantative changes in hyperstimulated mice at implantation period. Knowledge & Health 2015;10:31-9.[Persian]. doi: 10.1234/knh.v10i4.848

Hillier SG. Current concepts of the roles of follicle stimulating hormone and luteinizing hormone in folliculogenesis. Human Reprod 1994;9:188-91. doi: 10.1093/oxfordjournals.humrep.a138480

Salehnia M, Arianmanesh M, Beigi M. The impact of ovarian stimulation on mouse endometrium: a morphometrical study. IJRM 2006;4:7-11.

Gad A, Besenfelder U, Rings F, Ghanem N, Salilew-Wondim D, Hossain MM, et al. Effect of reproductive tract environment following controlled ovarian hyperstimulation treatment on embryo development and global transcriptome profile of blastocysts: implications for animal breeding and human assisted reproduction. Hum Reprod 2011;26:1693-707. doi: 10.1093/humrep/der110

Sibug RM, Helmerhorst FM, Tijssen AM, de Kloet ER, de Koning J. Gonadotrophin stimulation reduces VEGF(120) expression in the mouse uterus during the peri-implantation period. Hum Reprod 2002;17:1643-8. doi: 10.1093/humrep/17.6.1643

Artini PG, Fasciani A, Monti M, Luisi S, D'Ambrogio G, Genazzani AR. Changes in vascular endothelial growth factor levels and the risk of ovarian hyperstimulation syndrome in women enrolled in an in vitro fertilization program. Fertil Steril 1998;70:560-4. doi: 10.1016/s0015-0282(98)00221-0

Egbase P, Sharhan MA, Berlingieri P, Grudzinskas J. Serum oestradiol and progesterone concentrations during prolonged coasting in 15 women at risk of ovarian hyperstimulation syndrome following ovarian stimulation for assisted reproduction treatment. Human Reproduction 2000;15:2082-6.

Ishikawa K, Ohba T, Tanaka N, Iqbal M, Okamura Y, Okamura H. Organ-specific production control of vascular endothelial growth factor in ovarian hyperstimulation syndrome-model rats. Endocrine Journal 2003;50:515-25.

Published

2019-06-18

Issue

Section

Original Article(s)

How to Cite

The Effect of Progesterone Administration on the Number of Secondary Follicles of Ovulated Mice During Pre-Implantation. (2019). Knowledge and Health in Basic Medical Sciences, 14(1), Page:51-55. https://doi.org/10.22100/jkh.v14i1.2193

Most read articles by the same author(s)

<< < 14 15 16 17 18 19 20 21 22 23 > >>