تاثیر همزمان فعالیت تمرینی منظم و ویتامین D بر سطح آپپتوز و آنزیم های آنتی اکسیدانی بافت قلب در رت های نر قرار گرفته در معرض آب اکسیژنه

نویسندگان

  • Mehdi Pirooz1 1- گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.
  • Mohammad Ali Azarbayjani1 orcid http://orcid.org/0000-0002-3502-7487
  • Seyed Ali Hosseini 2 2- گروه فیزیولوژی ورزشی، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران.
  • Maghsoud Peeri1

DOI::

https://doi.org/10.22100/jkh.v13i2.1935

کلمات کلیدی:

استرس اکسیداتیو ، فعالیت تمرینی منظم ، مالون دی آلدئید ، SOD ، CAT

چکیده

مقدمه: اثرات بیولوژیک ترکیبات اکسیدکننده قوی در بدن انسان، تحت کنترل عوامل آنتی‌اکسیدان است. اختلال در عمل اندام‌ها ممکن است نتیجه واکنش‌هایی میان رادیکال‌های آزاد با غشاء سلول‌ها باشد. معلوم شده است که هدف اصلی رادیکال‌های اکسیژن، لیپیدهای غشاء سلول‌ها می‌باشد. هدف از مطالعه حاضر، بررسی آسیب‌پذیری سلول‌های میوکارد و ارزیابی آنزیم‌های آنتی‌اکسیدانی در بافت قلب رت‌های نر می‌باشد.

مواد و روش‌ها: 60 سر رت نر بالغ از نژاد ویستار با وزن 20±220 گرم و سن 8 تا 10 هفته به‌طور تصادفی براساس مداخلات استرس، ویتامین D و فعالیت تمرینی منظم به 10 گروه شش نفره شامل ویتامین D و فعالیت تمرینی منظم، گروه شاهد، آب اکسیژنه (H)، آب اکسیژنه دو برابر (2H)، آب اکسیژنه + ویتامین دی (HD)، آب اکسیژنه دو برابر + ویتامین دی (2HD)، آب اکسیژنه + تمرین (HE)، آب اکسیژنه دو برابر + تمرین (2HE)، آب اکسیژنه + تمرین + ویتامین دی (HDE)، آب اکسیژنه دو برابر + تمرین + ویتامین دی (2HDE) و نهایتاً دی متیل سولفوکساید + سالین(DMSO) تقسیم و به مدت 8 هفته تحت پروتکل مداخله قرار گرفتند. سپس غلظت آنزیم‌های آنتی‌اکسیدانی در بافت قلب اندازه‌گیری و با استفاده از تجزیه و تحلیل واریانس سه طرفه تحلیل شد. سطح معناداری برابر 05/0 در نظر گرفته شد.

نتایج: یافته‌ها نشان داد که تمرین باعث افزایش SOD و GPX شد اما افزایش کاتالاز معنادار نبود. همچنین از سوی دیگر تلفیق تمرین، ویتامین D و آب اکسیژنه تأثیر هم‌افزایی در مهار فعالیت پروتئین FAS داشت و در حفاظت و پیشگیری از عوارض ناشی از آپپتوز و التهاب میوکارد مؤثر بود.

نتیجه‌گیری: در رابطه با مصرف دوزهای مختلف H2O2 در مقایسه با گروه تمرین و D به تنهایی و یا گروه کنترل نشان داده شد که ترکیب تمرین استقامتی و D باعث افزایش معنادار و خیلی بیشتری در فعالیت آنزیم‌های SOD و GPX می‌شود.

بیوگرافی نویسنده

  • Mehdi Pirooz1، 1- گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.
    گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.

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