Cutaneous leishmaniasis Parasite Identification via PCR in the Infected Areas in Golestan Province

Authors

  • Farideh Tohidi دانشگاه علوم پزشکی گلستان- گروه انگل‌شناسی و قارچ‌شناسی- مربی
  • Afsaneh Borghaei دانشگاه علوم پزشکی گلستان- گروه پزشكي اجتماعي- استاديار

DOI:

https://doi.org/10.22100/jkh.v6i2.112

Keywords:

Cutaneous Leishmaniasis, Golestan province, PCR, Species

Abstract

 

Introduction: Classification and identification of parasite species leishmaniasis has been mainly based on the clinical features, geographical distribution and size of the parasite. Noting the limitations of this division, this study aimed to determine the epidemiological status and accurately determine the dominant parasite species based on molecular methods in the infected area in Golestan province as one of the foci of cutaneous leishmaniasis wet type (rural) in Iran.

Methods: This is a descriptive analytical study that was performed on the 63 patients suspected with ulcers of leishmaniasis and referred to the laboratories of rural health centers from 2004 to 2007. The data collection instrument was a questionnaire containing demographic variables, such as age, sex and residence as well as clinical variables such as shape, type, location, number and size of ulcers, previous history of oscar, the number and location of the scar. Along with the completion of questionnaires, direct samples from the wounds of patients and were taken and cultured in medium biphasic NNN (Novy, McNeill, Nicole).After the parasite growth in the culture ,then DNA was extracted and PCR experiments were performed.

Results: Statistically significant relationship was found between species of leishmania and ethnicity and gender (P<0.05). 100% (N=54) in the Turkmen ethnicity had leishmania major, 100% (N=3) of patients of Sistani ethnicity had leishmania tropica. The 33.3% (N=2) of Fars ethnicity patients had major species and 66.7% (N=4) had the tropica. Most patients were from rural areas of Gonbad. The disease was observed in both sexes. The number of males (57.1%) was higher than women (42.9%).

Conclusion: The study showed the dominant species of parasites in the infected area in Golestan Province was Leishmania major and its cutaneous leishmaniasis was of the rural type.

References

Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J of Clinical Microbiology 2006;44(4):1435-1439.

WHO. Control of the Leishmaniasis. Report of WHO Expert Committc. Tech Rep Ser 1990;793:130-133.

Gharavi MJ. Leishmaniasis. Clinical Protozoology. 3nd ed, Tabib 2004;190-245.[Persian].

Ihalamulla RL, Rajapaksa US, Karunaweera ND. Microculture for the isolation of leishmania modified to increase efficacy: a follow-up to a previous study. Ann Trop Med Parasitol 2006;100(1):87-89.

Hatam GR, Ardehali S, Motazedian MH, Sadjjadi SM, Fakoorziba MR. The methods of isolation and characterization of leishmania parasite. Shiraz: Loza press;2005.[Persian].

Edrissian GH, Rezaeian M, Ghorbani M, Keshavarz H, Mohebali M. Medical protozoology. 1nd ed Tehran University of Medical Sciences 2007:250-277.[Persian].

Nazari F. Survey of cutaneous leishmaniasis and determination of reservoir in Minoodasht of Golestan province [dissertation]. Tehran University of Medical Sciences. Health Faculty 1999.[Persian].

Tohidi F, Kazemi B, Abaszadegan MR. Cloning of leishmania pteridine reductase gene. Proceeding of the 4th National Iranian Congrees of Parasitology Parasitology & Parasite Diseases. Mashhad University of Medical Sciences 2003.[Persian].

Saunders GC,Parkers HC.Analytical Molecular Biology(Quality and Validation). Uk:Trowbridge,Wiltshire;1999.

Piarroux R, Gambareli F, Dumon H, Fontes M, Dunan S, Mary C, et al. Comparison of PCR with direct examination of bone marrow aspiration, myloculture and serology for diagnosis of visceral Leishmaniasis in immunocom promised Patients. J Clin Microbiol .1994;32(3):746-749.

Marin f, Garcia J, Penarrubia MPG, Penalver J. Cultivation of leishmania: Comparison of different media for promastigote cultivation. Ann Trop Med Parasitol 1982;76(6):607-613.

Mohajeri M, Bolorsaz M, Shamsian SAA. Prevalence of cutaneous Leishmaniasis in secondery school students in Mashhad, Iran. Journal of Mashhad University of Medical Sciences 2001;44(72):54-60.[Persian].

Rajabi P. Comparison between two sampling FNAC and scraping in microscopic diagnosis of cutaneous leishmaniasis. J of Research in Medical Sciences;4(1):3-6.[Persian].

Silveira T.G, Arraes SM, Bertolini DA, Teodoro U, Lonardoni MV, Roberto AC, et al. The laboratory diagnosis and epidemiology of cutaneous leishmaniasis in parana state, southern Brazil. Rev Soc Bras Med Trop 1999;32(4):413-23.

Tohidi F,Kazemi B,Mohajery M. Survey epidemiological status of cutaneous Leishmaniasis in Mashhad. Proceeding of 5th National Iranian Congress of Parasitology;Tehran:Iran

Hamzavi Y, Mohebali M, Edrisian GH, Foruzani A. An epidemiological study of cutaneous Leishmaniasis (human being and animal reservoir) in dashtestan and dashti districts, Bushehr Province, Iran, 1998-99. Iranian Journal of Public Health 2000;1 (29):177-190.[Persian].

Yaghoobi Ershadi MR, Jafari R. An epidemiological study of cutaneous leishmaniasis in Nikabad town. Proceeding of 3th National Iranian Congress of Parasitology; Sari, Iran. Mazenderan University of Medical Sciences;2000.[Persian].

Soleimani M, Shahi M, Madani A. Study of cutaneous leishmaniasis in Hormozgan province (1999). Proceeding of 3th National Iranian Congress of Parasitology; Sari, Iran. Mazenderan University of Medical Sciences;2000.[Persian].

Gurel MS, Ulukanligil M, Ozbilge H. Cutaneous Leishmaniasis in sanliufa: epidemiologic and clincal features of the last four years 1997-2000. Int J Dermatol 2002;41(1):32-7.

Yaghoobi Ershadi MR, Hanafi AA, Akhavan AA, Zahraei Ramazani AR, Mohebali M. Cutaneous leishmaniasis in Ardestan town. Hakim Research Journal 1999;3(1):206-214.[Persian].

Sedghiyani SH, Hanafi Bejed AA, Mehdipoor D. The study of cutaneous leishmaniasis in patients reffered to medical of center in Tehran south (1998-1999). Proceeding of 3rd National Iranian Congrees of Parasitology; Sari, Iran. Mazenderan University of Medical Sciences;2000.[Persian].

Sadeghinejad B. The Survey of cutaneous leishmaniasis in patients referred to medical of center in khozestan province (1998-1999). Proceeding of 3rd National Iranian Congress of Parasitology;Sari, Iran. Mazandaran University of Medical Sciences;2000.[Persian].

Uzun S, Uslular C,Yucel A, acar MA, Ozpoyraz M, Memisoglu HR. Cutaneous leishmaniasis: evaluation of 3074 cases in the cukurova region of Turkey. Br J Dermatol 1999;140(2):347-50.

Doroodgar A, Mahbobi S, Nemetian M, Sayyah M, Doroodgar M. An epidemiological study of cutaneous Leishmaniasis in Kashan (2007-2008)‎. Koomesh 2009;10(3):177-184.[Persian].

Sharifi I, Ardehali S, Motazedian MH. Diagnosis, identification and characterization of leishmania isolates in school children in Bam, southeastern Iran. Iranian J Medical Sciences 1997;23(1&2):82-88.

Daneshvar Farzanegan P, Baghaei M, Janghorbani M, Karimi J. The survey of cutaneous leishmaniasis in patients reffered to medical of center in barkhar and myme, Isfahan, Iran (1998-2000). Proceeding of 10th National Iranian Congress of Infection and Tropical Diseases. Tehran 2001.[Persian].

Published

2011-12-17

Issue

Section

Original Article(s)

How to Cite

Cutaneous leishmaniasis Parasite Identification via PCR in the Infected Areas in Golestan Province. (2011). Knowledge and Health in Basic Medical Sciences, 6(2), 26-31. https://doi.org/10.22100/jkh.v6i2.112

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