Effect of Berberine Hydrochlorid on Testicular Damage in Streptozotocin-Induced Diabetic Rats
DOI:
https://doi.org/10.22100/jkh.v7i3.145Keywords:
Diabetes, Berberine, Spermatogenesis, Germinal layer, Ssertoli cell, Testes injuryAbstract
Introduction: Diabetes mellitus mediated by oxidative stress creates serious metabolic disorders in testicles. This study aimed to demonstrate the antioxidant effect of berberine hydrochlorid on testicular damage in streptozotocin induced diabetes in male rats.
Methods: Thirty two, two-month-old male wistar rats (220- 240 g body weight) were divided into 6 groups: control group, berberine -treated nondiabetic group (100 mg/kg), berberine -treated nondiabetic group (50 mg/kg), diabetic group, berberine –treated diabetic group (100 mg/kg) and berberine –treated diabetic group (50 mg/kg).Diabetes was induced by STZ intraperitoneally injection (50 mg/kg). Berberine was gavaged at the dose of 50, 100 mg/kg for 6 weeks. Testicular damage was examined by using hematoxylin and eosinstaining. Blood biochemical factors also were measured.
Results: Diabetes mellitus mediated oxidative stress and free radical production, significantly reduced spermatogenic and Sertoli cells, decreased spermatogenesis and sperm motility and treatment with berberine hydrochlorid, improved this effects. Berberine increases sperm count and motility, spermatogenic cells and sertoli cells. Furthermore in thraputic groups we observed increas in seminiferous tubule diameter and decreas in basal laminal thickness.
Conclusions: These results suggest that berberine hydrochlorid has beneficial effects on testicular damage in adult diabetic rats, probably berberine induce this effects by decreasing blood glucose concentration and preventing oxidative stress.
References
Guyton & Hall, Medical physiology ,12th ed, Translated to Persian by: Sepehri H, Rastkar A, Ghasemi K. Andishe Rafia pub;2011. p.1194.[Persian].
Pop-Busui R, Anders S, Stevens M. Diabetic neuropathy and oxidative stress. Diabetes Metab Res Rev 2006;22:257-273.
Jawa A, Kcomt J, Fonseca VA. Diabetic noropathy and retinopathy. Med Clin North Am 2004, 88 (4); 1001-36.
Jakus V, Rietbrock N. Advanced glycation end-products and the progress of diabetic vascular complications. Physiol Res 2004;53:131-42.
Amaral S, Moreno AJ, Santos MS, Seic R¸ Ramalho-Santos J. Effects of hyperglycemia on sperm and testicular cells of Goto-Kakizaki and streptozotocin-treated rat models for diabetes. Theriogenology 2006;66(9):2056-2067.
Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. Int J Androl 2006; 29(4):482-8.
Ning G, Hong J, Bi Y, Gu W, Zhang Y, Zhang Z, Huang Y, Wang W, Li X. Progress in diabetes research in China. Journal of Diabetes 2009;1(3):163-172.
Soudamani S, Yuvaraj S, Rengarajan S, Sivakumar R, Malini T, Balasubramanian K. Effects of streptozotocin diabetes and insulin replacement on androgen and estrogen receptor concentrations in the epididymis of wistar rats. JER 2006;10:59-61.
Arikawe AP, Daramola AO, Odofin AO, Obika LF. Alloxan-induced and insulin-resistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats. Afr J Reprod Health 2006:10(3):106-13.
Cederberg J, Eriksson UJ. Antioxidative treatment of pregnant diabetic rats diminishes embryonic dysmorphogenesis. Birth Defects Res A Clin Mol Teratol 2005;73:498-505.
Abdolah nejad A, Gol A, Dabiri Sh, Javadi AR. Effect of garlic on testicular damage in streptozotocin-induced diabetic rats. endocrine glands & metabolism Journal of Shahid Beheshti University of Medical Sciences and Health Services 2010;11(4):443-53.[Persian].
Kim ST, Moley KH. Paternal effect on embryo quality in diabetic mice is related to poor sperm quality and associated with decreased glucose transporter expression. Reproduction 2008;136(3):313-322.
Yagihashi S, Yamagishi SI, Wada Ri R, Baba M, Hohman TC, Yabe-Nishimura C. Neuropathy in diabetic mice overexpressing human aldose reductase and effects of aldose reductase inhibitor. Brain 2001;124:2448-2458.
Jakus V, Rietbrock N. Advanced glycation end-products and the progress of diabetic vascular complications. Physiol Res 2004;53:131-42.
Pop-Busui R, Marinescu V, Van Huysen C. Dissection of metabolic, vascular, and nerve conduction interrelationships in experimental diabetic neuropathy by cyclooxygenase inhibition and acetyl-L-carnitine administration. Diabetes 2002;51:2619-2628.
Kellogg A, Pop-Busui R. Peripheral nerve dysfunction in experimental diabetes is mediated by cyclooxygenase-2 and oxidative stress. Antioxid Redox Signal 2005;17:1521-1529.
Nishikawa T, Edelstein D, Du XL. Normalizing mitochondrial super oxide production blocks three pathways of hyperglycemic damage. Nature 2000;404:787-790.
Qiong Liu, Lili Chen, Lihong Hu, Yuewei Guo, Xu Shen. Small molecules from natural sources, targeting signaling pathways in diabetes. Biochimica et Biophysica Acta 2010:1799(10-12):854-865.
Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. Sexual behavior, sperm quantity and quality after short –term streptozotocin -induced hyperglycemia in rats. Int J Androl 2006;29:482-488.
Vuddanda PR, Chakrabory S, Singh S. Berberine: a potential phytochemical with multispectrum therapeutic activities. Expert Opin Investing Drugs 2010;10:1297-1307.
Kong WJ, Zhang H, Song DQ. Berberine reduce insulin resistance through protein kinase C-dependent up-regulation of insulin receptor expression. Metabolism 2009;58:109-19.
Zhou J, Zhou S, Tang J, Zhang K, Guang L, Huang Y, et al. Protective effect of berberine on beta cells in streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. European Jornal of Pharmacology 2009;606:262-268.
Sharmaa B, Salunkea R, Balomajumderb C, Daniela S, Roy P. Anti-diabetic potential of alkaloid rich fraction from Capparis decidua on diabetic mice. J Ethnopharmacol 2010;127: 457-462.
Wang HD, Lu DX, Qi RB. Therapeutic strategies targeting the LPS signaling and cytokines. Pathophysiology 2009;16:291-296.
Matteucci E, Giampietro O. Proposal open for discussion: defining agreed diagnostic procedures in experimental diabetes research. J Ethnopharmacol 2008;115:163-172.
Barrera G, Pizzimenti S, Dianzani MU. Lipid peroxidation: control of cell proliferation, cell differentiation and cell death. Molecular Aspects of Medicine 2008;29:1-8.
Liu J, Shen W, Zhao B, Wang Y, Wertz K, Weber P, et al. Targeting mitochondrial biogenesis for preventing and treating insulin resistance in diabetes and obesity: Hope from natural mitochondrial nutrients. Adv Drug Deliv Rev 2009;61:1343-1352.
Aitken R. John , Roman Shaun D. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev 2008;1(1):15-24.
Luca G, Calvitti M, Baroni T. Sertoli cell-induced adult rat islet beta-cell mitogenesis: causative pathways. Diabetes Nutr Metab 2003;16(1):1-6.
Rossi GL, Bestetti G. Morphological changes in the hypothalamic-hypophyseal- gonadal axis of male rats after twelve months of streptozotocin-induced diabetes. Diabetologia 1981;21(5):476-81.
Baccetti B, La Marca A, Piomboni P, Capitani S, Bruni E, Petraglia F, et al. Insulin-dependent diabetes in men is associated with hypothalamo-pituitary derangement and with impairment in semen quality. Hum Reprod 2002;17:2673-7.
Liang Shen, Hong-Fang Ji. The mechanisms of ROS-photogeneration by berberine, a natural isoquinoline alkaloid. J Photochem Photobiol B 2010;99:154-156.
Zhang XD, Ren HM, Liu L. Effects of different dose berberine on hemodynamic parameters and [Ca2+]i of cardiac myocytes of diastolic heart failure rat model. Zhongguo Zhong Yao Za Zhi 2008;33(7):818-21.
Liu W, Liu P, Tao S, Deng Y, Li X, Lan T. Berberine inhibits aldose reductase and oxidative stress in rat mesangial cells cultured under high glucose. Arch Biochem Biophys 2008;475:128-134.
Tan Y, Tang Q, Hu BR, Xiang JZ. Antioxidant properties of berberine on cultured rabbit corpus cavernosum smooth muscle cells injured by hydrogen peroxide. Acta Pharmacol Sin 2007; 28:1914-1918.
BalasinornNH, Dsouzar R, Upadhyayr, Anology I. Effect of high intratesticular estrogen on spermatogenesis. International Journal of Fertility and Sterility 2010.
Gu Yan, Zhang Yifei, Shi Xianzhe, Li Xiaoying, Hong Jie, Chen Jing, et al. Effect of traditional Chinese medicine berberine on type 2 diabetes based on comprehensive metabonomics. Talanta 2010;81:766-772.
Zhang Hui, Tan Conge, Wang Hongzhan, Xue Shengbo, Wang Miqu. Study on the history of traditional chinese medicine to treat diabetes. European Journal of Integrative Medicine 2010;2:41-46.
Sharma B, Salunke R, Balomajumder C, Daniel S, Roy P. Anti-diabetic potential of alkaloid rich fraction from Capparis decidua on diabetic mice. Journal of Ethnopharmacology 2010;127:457-462.
Sun Y, Xun K, Wang Y, Chen X. A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs. Anti-Cancer Drugs 2009;20:757-769.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
