Investigation of Nitrite and Nitrate Levels in Some Summer Vegetables Cultivated in Bastam Region-Shahroud-Iran

Authors

  • Aliakbar Roudbari - Environmental and Occupational Health Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. orcid https://orcid.org/0000-0002-9672-3646
  • Marjan Ghanbarian - Environmental and Occupational Health Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. orcid https://orcid.org/0000-0003-1387-8528
  • Allah-Bakhsh Javid - Environmental and Occupational Health Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. orcid https://orcid.org/0000-0002-2037-9252
  • Maral Afshari - Student Research Committee, School of Public Health, Shahroud University of Medical Sciences, Shahroud, Iran.

DOI:

https://doi.org/10.22100/jkh.v17i3.2704

Abstract

Introduction: Excessive intake of nitrite and nitrate threatens human health. The aim of this study was to determine the levels of nitrite and nitrate in some summer vegetables cultivated in Bastam region of Shahroud.

Methods: the study was conducted in the summer. The study area was divided and a total of 25 summer vegetables, 15 soil samples, and three water samples were measured to determine the levels of nitrate and nitrite according to the Standard instruction (No. 4106).

Results: The total average of nitrate in cucumber, tomato, potato, eggplant and onion  was 850.1 ± 65.2, 642.1± 25.4, 48.6 ± 2.5, 14.8 ± 4.5, 791.3±52.1 mg/kg fresh weight,  respectively and this  level for nitrite was equal to 9.23 ± 0.1, 7.2 ± 0.8, 0.52 ± 0.04, 0.9± 0.05, 5.3±0.32 mg/kg fresh weight that all values were less than the standard  level.

The  level of nitrate and nitrite in the soil was 1250.68 ±562.2 and 98.1 ± 15.2 mg/kg soil weight, respectively. The  level of nitrate and nitrite in the water was 5.68±2.2 and 0.23±0.2 mg/l, respectively.

Conclusion: Due to the high consumption of vegetables and summer vegetables in summer and spring, which are also the fertilizing seasons for plants and shrubs, it is recommended that managers and farmers pay more attention to the process of cultivation and fertilization of agricultural products, as well as frequent monitoring of the level of nitrite and nitrate in the country's vegetables.

References

Cammack R, Joannou CL, Cui X-Y, Torres Martinez C, Maraj SR, Hughes MN. Nitrite and nitrosyl compounds in food preservation. Biochim Biophys Acta Bioenerg 1999;1411:475-88. doi:10.1016/S0005-2728(99)00033-X

Merusi C, Corradini C, Cavazza A, Borromei C, Salvadeo P. Determination of nitrates, nitrites and oxalates in food products by capillary electrophoresis with pH-dependent electroosmotic flow reversal. Food Chem 2010;120:615-20. doi: 10.1016/j.foodchem.2009.10.035

Hsu J, Arcot J, Alice Lee N. Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary intake in Australians. Food Chem 2009;115:334-9. doi:10.1016/j.foodchem.2008.11.081

Ward MH, deKok TM, Levallois P, Brender J, Gulis G, Nolan BT, et al. Workgroup report: Drinking-water nitrate and health--recent findings and research needs. Environ Health Perspect 2005;113:1607-14. doi: 10.1289/ehp.8043

Research I of S and I. , test and measurement of nitrite and nitrate in fruit and vegetable products by their measured molecular spectrum. 1998.

FAO. setting maximum levels for certain contaminants in foodstuffs. 2006.

Commission Regulation (EU) No 1258/2011. Amending Regulation (EC) No 1881/2006 as regards maximum levels for nitrates in foodstuffs. Vol. 320, Official Journal of the European Union. 2011. doi: 1881/2006

ISIRI. Nitrat and Nitrit measurement in Food. 1998.

Jafari R FA and azizzade A. The amount of nitrate and nitrite in some vegetables produced in Esfahan city. J Esfahan Univ Med Sci 2000;3:123-5.

Pirsaheb, M. Sharafi, K., Moradi M. A survey on nitrite and nitrate levels in vegetables and cucurbits cultivated in northern and western plains of Kermanshah city in 2012. Food Hygine 2013;3:77-8.

Shahlaei A, Ansari NA, Dehkordie FS. Evaluation of nitrate and nitrite content of Iran southern (Ahwaz) vegetables during winter and spring of 2006. Asian J Plant Sci 2007;6:1197-203. doi: 10.3923/ajps.2007.1197.1203

Chung SY, Kim JS, Kim M, Hong MK, Lee JO, Kim CM, et al. Survey of nitrate and nitrite contents of vegetables grown in Korea. Food Addit Contam 2003;20:621-8. doi: 10.1080/0265203031000124146.

Ayaz A, Topcu A, Yurttagul M. Survey of nitrate and nitrite levels of fesh vegetables in Turkey. Journal of Food Technology 2007;p. 177–9.

Javid A, Roudbari AA, Mashayekh-Salehi A, Ghanbarian M, Ghanbarian M. The effect of conventional water disinfection methods on nitrate level. Int J Water 2020;14:1-13. doi: 10.1504/IJW.2020.112710

Joossens J V, Hill MJ, Elliott P, Stamler R, Lesaffre E, Dyer A, et al. Dietary salt, nitrate and stomach cancer mortality in 24 countries. European Cancer Prevention (ECP) and the INTERSALT Cooperative Research Group. Int J Epidemiol 1996;25:494-504. doi: 10.1093/ije/25.3.494

Stroka J. Food Additives & Contaminants: Part A: Chemistry, Analysis, Control, Exposure & Risk Assessment. Foreword. Vol. 28, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment. England 2011;p.259. doi: 10.1080/19440049.2011.561599

Bartsch H, Ohshima H, Shuker DEG, Pignatelli B, Calmels S. Exposure of humans to endogenous N-nitroso compounds: implications in cancer etiology. Mutat Res Genet Toxicol. 1990;238:255-67. doi: 10.1016/0165-1110(90)90017-6

Ishaq S, Nunn L. Helicobacter pylori and gastric cancer: a state of the art review. Gastroenterol Hepatol from Bed to Bench 2015;8:S6-14.

Figura N, Marano L, Moretti E, Ponzetto A. Helicobacter pylori infection and gastric carcinoma: Not all the strains and patients are alike. World J Gastrointest Oncol 2016;8:40-54. doi: 10.4251/wjgo.v8.i1.40.

Liu KS-H, Wong IO-L, Leung WK. Helicobacter pylori associated gastric intestinal metaplasia: Treatment and surveillance. World J Gastroenterol 2016;22:1311-20. doi: 10.3748/wjg.v22.i3.1311

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Published

2022-09-26

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Original Article(s)

How to Cite

Investigation of Nitrite and Nitrate Levels in Some Summer Vegetables Cultivated in Bastam Region-Shahroud-Iran. (2022). Knowledge and Health in Basic Medical Sciences, 17(3), Page:33-39. https://doi.org/10.22100/jkh.v17i3.2704