The Investigating the Effect of Gracilaria Corticata Algae Extract On Blood Supply and Fibrosis of Infected Rat Skin Wounds
Authors
-
Hooman Akasheh
- Dept. of Physiology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran.
https://orcid.org/0000-0001-9024-9640
-
Iraj Mirzaii-Dizgah
- Dept. of Physiology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran. , Infections Diseases Research Center
https://orcid.org/0000-0003-3488-6842
-
Seyyed Javad Hosseini Shokouh
- Infections Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran.
https://orcid.org/0000-0002-1392-1736
DOI:
https://doi.org/10.22100/jkh.v18i2.2936Abstract
Introduction: Wound healing depends on angiogenesis and collagen production by fibroblasts. Seaweeds are among the organisms that are of interest today for healing wounds. This study examines the effect of seaweed called Gracilaria corticata to increase angiogenesis and fibrosis of infectious skin wounds.
Methods: Eighty male rats that participated in this experiment were maintained according to the Laboratory Guidelines for Animal Care. The animals were divided into four groups (20 rats in each group), and a square wound on the back skin was performed on all rats. The time points were 3, 7, 14, and 21 days after the surgical procedure. After creating a wound, an aliquot of 50 µL of suspension containing 2x108 CFU/mL of Staphylococcus bacteria was inoculated onto each wound. The groups were divided into control (no treatment), zinc oxide (control), 3%, and 7% ointment (algae treatment). The samples were placed in 10% buffered formalin and then in the tissue processing and staining with trichrome. Trichrome staining is used to show collagen fibers and the degree of fibrosis. On the other hand, VEGF and TGF-Β antibodies were used for immunohistochemical evaluation.
Results: The use of algae increased the occurrence of VEGF and TGF-Β more than the administration of zinc oxide.
Conclusion: The use of algae has a strengthening effect, especially in the form of ointment, on the healing of infected wounds. It can be related to more blood supply and, as a result, more immunity in the wound, along with antimicrobial effects.
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