Investigation of the Reliability of the Blood Markers in Human Identity Recognition by DNA Finger Printing
DOI:
https://doi.org/10.22100/jkh.v9i1.346Keywords:
Blood groups, Blood relation identification, DNA finger printing recognitionAbstract
Introduction: Determination of blood groups is the first step in the approval or rejection of blood relation between the two individuals for identity recognition. The aim of this study is to investigate the reliability of common blood grouping systems in DNA finger printing recognition.
Methods: In this study, blood samples were obtained from 300 individuals belonging to 150 families. Then, DNA of each individual was purified and DNA finger printing was performed for 10 STR regions using ABI set. Seven human blood group systems including ABH, RH, Kidd, Kell, Mns, Lutheran and p1 were determined with agglutination methods, and the results were compared with DNA sequencing results of the ABI sequencing machine.
Results: 300 randomly selected individuals were studied, in 256 cases. There was no difference between the blood grouping and DNA typing results; in 44 cases, the blood relation was approved by DNA typing but antigenic discord was observed in RH, MNS and ABH antigens. Among the blood grouping systems, RH, MNS and ABH,with the error scale of 30.7% (P=0.001), 20.54% (0.04) and 17.6% (P=0.005) respectively, were found to be the weakest systems for human identity recognition.
Conclusion: Among the blood grouping systems, RH, MNS and ABH are the weakest markers for blood relation identification, and DNA finger printing must be used as a supplementary test to confirm their results.
References
Whitaker JP, Clayton TM, Urquhart AJ, Millican ES, Downes TJ, Kimpton CP, et al. Short tandem repeat typing of bodies from a mass disaster: high success rate and characteristic amplification patterns in highly degraded samples. Biotechniques 1995;18:670-7.
Budimlija ZM, Prinz MK, Zelson-Mundorff A, Wiersema J, Bartelink E, MacKinnon G, et al. World Trade Center human identification project: experiences with individual body identification cases. Croat Med J 2003;44:259-63.
Bryant Neville. Disputed paternity, New York: Bian C;1980.
Vengelen– Tyler V. Technical manual. 12th ed. Ehesda: AABB; 1996.
Butler John M. Forensic DNA typing. New York: Academic Press. 2001;28-30.
Sprecher CJ, Puers C, Lins AM, Schumm JW. General approach to analysis of polymorphic short tandem repeat Loci. Biotechniques 1996;20(2):266-76.
Kalmes R, Huret JL. Hardy-wienberg model. Atlas Genet Cytogenet Oncol Haematol, 2001.
Wiener AS, Wexler IB. Heredity of the blood groups. New York: Grune & Stratton Press; 1958, 16-18.
Tong GTF, Pang TC. The frequency of the ABO blood groups amongst the chinese population in Hong Kong. The bulletin of the Hong Kong Chinese medical association; 1965.
Gill P, Jeffreys Aj, Werett Dj. Forensic application of DNA fingerprints. Nature 1985;318:577-579.
Cerda-Flores RM, Barton SA, Marty Gozzalez Lf, Rivas F, Chkraborty R. Estimation of non paternity in the Mexican Population of Nuevo Leon: a validation Study with blood group markers. Am J phys Anthropol 1999;109:281-293.
FaRHud DD, Eftekhari A. Blood Groups distribution in Iran. Iranian J Publ Health 1994;23:1-4.
Guy LR, Husestis DW, Wilson LR. Technical methods and Procedures. 4th ed. Chicago: American Association of Blood banks;1966.
Issitt PD, Anstee DJ. Applied blood group serology. 4th ed. DuRHam: Montgomery Scientific Publications; 1998.
Lomas Francis C. The potential of monoclonal antibodies to RH,MNS, and other blood group antigens. Arlington: AABB; 1997.
Harmening DM. Modern blood banking and transfusion. 4th ed. Philadelphia: FA Davis; 1999.
Rohland N, Hofreiter M. Comparison and optimization of ancient DNA extraction. Biotechniques 2007;42:343-52.
Huffine E, Crews J, Kennedy B, Bomberger K, Zinbo A. Mass identification of persons missing from the break-up of the former Yugoslavia: structure, function, and role of the International Commission on Missing Persons. Croat Med J 2001;42:271-5.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
