Effect of Leukocytes Transfer on the Induction of Liver Damage after Renal Ischemia- Reperfusion in Inbred Mice
DOI:
https://doi.org/10.22100/jkh.v7i2.80Keywords:
Renal ischemia-reperfusion, Liver damage, Leukocytes, Oxidative stress.Abstract
Introduction: Renal ischemia-reperfusion (IR) induces organ damage in remote organs such as liver, brain and lung. The aim of this study was to assess the role of leukocytes in the induction of liver damage after renal IR injury.
Methods: Inbred mice were subjected to either sham operation or bilateral renal IR injury (60 min ischemia followed by 3h reperfusion). Mice were then anesthetized for collection of leukocytes by heart puncture. Isolated leukocytes were transferred to two other groups: intact recipient mice that received leukocytes from IR mice and intact recipient mice that received leukocytes from sham-operated control mice. After 24h, recipient mice were anesthetized and blood and hepatic samples were collected.
Results: Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and hepatic malondialdehyde (MDA) increased significantly in intact recipient mice that received leukocytes from IR mice in comparison to intact recipient mice receiving leukocytes from sham-operated control mice. In addition, loss of normal liver architecture, cytoplasmic vacuolization and focal infiltration of leukocytes were observed.
Conclusion: These results suggest that leukocytes are one of the possible factors that contribute to liver damage after renal IR injury and this damage is partly due to the induction of oxidative stress.References
Hassoun H, Grigoryev DN, Lie ML, Liu M, Cheadle C, Tuder RM, et al. Ischemic acute kidney injury induces a distant organ functional and genomic response distinguishable from bilateral nephrectomy. Am J Physiol-Renal Physiol 2007;293(1):F30-40.
Golab F, Kadkhodaee M, Zahmatkesh M, Hedayati M, Arab H, Schuster R, et al. Ischemic and non-ischemic acute kidney injury cause hepatic damage. Kidney Int 2009;75(8):783-92.
Zhang Z, Wang S, Huang X, Min WP, Sun H, Liu W, et al. NK cells induce apoptosis in tubular epithelial cells and contribute to renal ischemia-reperfusion injury. J Immunol 2008;181(11):7489-98.
Lee HT, Kim M, Kim M, Kim N, Billings FT 4th, D'Agati VD, et al. Isoflurane protects against renal ischemia and reperfusion injury and modulates leukocyte infiltration in mice. Am J Physiol-Renal 2007;293(3):F713-22.
Rabb H, Daniels F, O'Donnell M, Haq M, Saba SR, Keane W, et al. Path physiological role of T lymphocytes in renal ischemia-reperfusion injury in mice. Am J Physiol-Renal 2000;279(3):525-31.
Mizutani A, Okajima K, Uchiba M, Isobe H, Harada N, Mizutani S, et al. Antithrombin reduces ischemia/reperfusion-induced renal injury in rats by inhibiting leukocyte activation through promotion of prostacyclin production. Blood 2003;101(8):3029-36.
Liu M, Liang Y, Chigurupati S, Lathia JD, Pletnikov M, Sun Z, et al. Acute kidney injury leads to inflammation and functional changes in the brain. J Am Soc Nephrol 2008;19(7):1360-70.
Kelly K. Distant effects of experimental renal ischemia/reperfusion injury. J Am Soc Nephrol 2003;14(6):1549-58.
Hoke TS, Douglas IS, Klein CL, He Z, Fang W, Thurman JM, et al. Acute renal failure after bilateral nephrectomy is associated with cytokine-mediated pulmonary injury. J Am Soc Nephrol 2007;18(1):155-64.
Zarbock A, Schmolke M, Spieker T, Jurk K, Van Aken H, Singbartl K. Acute uremia but not renal inflammation attenuates aseptic acute lung injury: a critical role for uremic neutrophils. J Am Soc Nephrol 2006;17(11):3124-31.
Miyazawa S, Watanabe H, Miyaji C, Hotta O, Abo T. Leukocyte accumulation and changes in extra-renal organs during renal ischemia reperfusion in mice. J Lab Clin Med 2002;139:269-78.
Serteser M, Koken T, Kahraman A, Yilmaz K, Akbulut G, Dilek ON. Changes in hepatic tnf-[alpha] levels, antioxidant status, and oxidation products after renal ischemia/reperfusion injury in mice. J Surg Res 2002;107:234-40.
Kadkhodaee M, Golab F, Zahmatkesh M, Ghaznavi R, Hedayati M, Arab HA, et al. Effects of different periods of renal ischemia on liver as a remote organ. World J Gastroenterol 2009;15(9):1113-8.
Golab F, Kadkhodaee M, Zahmatkesh M, Ghaznavi R, Arab HA, Seifi B, et al. Hepatic changes during various periods of reperfusion after induction of renal ischemia in rats. Transpl P 2009;2749-50.
Esterbauer H, Cheeseman K. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Method Enzymol 1990;186:209-20.
Harada N, Okajima K, Murakami K, Usune S, Sato C, Ohshima K, et al. Adenosine and selective A2A receptor agonists reduce ischemia/reperfusion injury of rat liver mainly by inhibiting leukocyte activation. J Pharmacol Exp Ther 2000;294(3):1034-42.
Lipton B, Delcarpio J, McDonough K. Effects of endotoxin on neutrophil-mediated ischemia/reperfusion injury in the rat heart in vivo. Exp Biolo Med 2001;226(4):320-7.
Asaga T, Ueki M, Chujo K, Taie S. JTE-607, an inflammatory cytokine synthesis inhibitor, attenuates ischemia/reperfusion-induced renal injury by reducing neutrophil activation in rats. J Biosci Bioeng 2008;106:22-6.
Chujo K, Ueki M, Asaga T, Taie S. Atrial natriuretic peptide attenuates ischemia/reperfusion-induced renal injury by reducing neutrophil activation in rats. The Tohoku J Exp Med 2008;215:257-66.
Burne-Taney M, Yokota-Ikeda N, Rabb H. Effects of combined T-and B-cell deficiency on murine ischemia reperfusion injury. Am J Transplant 2005;5:1186-93.
Burne-Taney MJ, Ascon DB, Daniels F, Racusen L, Baldwin W, Rabb H. B cell deficiency confers protection from renal ischemia reperfusion injury. J Immunol 2003;171(6):3210-5.
Li L, Huang L, Sung SS, Lobo PI, Brown MG, Gregg RK, et al. NKT cell activation mediates neutrophil IFN-γ production and renal ischemia-reperfusion injury. J Immunol 2007;178:5899.
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