The Simultaneous Effect of Regular Exercise and Vitamin D on NF-κBp65 levels in Male Rats Exposed to Hydrogen Peroxide

Abstract

Introduction: Oxidative stress and inflammation are involved in common signaling pathways and play central role in heart functions and disorders. Factor kappa-B p65 (NF-κBp65), is an important marker of myocardial inflammation. This study investigated the combination of regular exercise (E), vitamin D (VD) and oxidative stress induced on the levels of NF-κBp65 in the myocardium.

Methods: 60 adult male rats of the Wistar race were randomly divided into 10 groups: control; H₂O₂; 2H₂O₂; H₂O₂+VD₃; 2H₂O₂+VD₃; H₂O₂+E; 2H₂O₂+E; H₂O₂+VD₃+E; 2H₂O₂+VD₃+E and dimethyl sulfoxide + saline that were under the intervention protocol for 8 weeks. An ELISA kit used to measure NF-κBp65 concentration. Data were analyzed three-way ANOVA. Significant level was set at 0.05.

Results: The results showed the Exercise had a decrease effect on NF-κBp65 protein concentration (P=0.049). Also, high concentration of H₂O₂ increased the NF_κBp65 protein concentration (P=0.013). On the other hand, the interaction of E and VD had decreasing effect on NF-κBp65 protein concentration (P=0.004). However, VD and E interaction with H₂O₂ were not significant. Coincidence of three interventions E, H₂O₂ and VD compared to the combined effect of each one, had a decreasing effect on the protein concentration of NF-κBp65 (P=0.038).

Conclusion: It seems that regular exercise activity, VD antioxidant and exogenous induction of oxidative stress can be used to preconditioning and reduce myocardial inflammation in high oxidative stress conditions.