The Effect of an Interval Exercise Period (HIIT) on MTNR1B Gene Expression, Insulin and Glucose Levels in Type 2 Diabetic Rats
Authors
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Mohammad Rashidi*1
1- Dept. of Exercise Physiology, Semnan Branch, Islamic Azad University, Semnan, Iran.
http://orcid.org/0000-0002-2675-9539
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Mojtaba Eizadi2
2- Dept. of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
http://orcid.org/0000-0003-1989-692X
DOI:
https://doi.org/10.22100/jkh.v14i1.2151Keywords:
MTNR1B expression, Interval training, Rat with type II diabetes, Glucose level and insulin level.Abstract
Introduction: MTNR1B genes expression is associated with increase of the of type 2 diabetes . The objective of this study was to evaluate the effect of 12 weeks of high intensity intermittent exercise on MTNR1B gene, glucose and insulin levels in diabetic rats
Methods: In this study, 30 male Wistar rats weighing 220± 20g were divided into three groups control, diabetic and HIIT/diabetic groups. Administration of streptozotocin (STZ) and nicotinamide (NA) induced experimental diabetes in the rat. Interval Exercise (HIIT) performed for 12 weeks (5 times/30 mintes). The glucose concentration examined using oxidase colorimetric method, insulin by ELISA and gene expression was measured by RT-Real time PCR. All statistical analysis performed using s SPSS.
Results: Exercise program decreased fasting blood glucose and serum insulin in diabetic group compared to the control (P=0.001). Intensive interval training decreased MTNR1B gene expression in pancreatic tissue compared to diabetic control group (P=0.023). There was a significant correlation between the expression of MTNR1B and serum insulin (P=0.039, r=-0.84)
Conclusion: The results showed that twelve weeks of high intensity intermittent exercise decrease blood glucose and increase serum insulin and decrease of MTNR1B gene expression in pancreatic tissue.
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