Curcumin prevents 6-OHDA Induced Cell Death and ERK disruption in Human Neuroblastoma Cells
Authors
-
Maryam Moosavi1,2
1- Shiraz Neuroscience Research Center, Shiraz University of Medical sciences, Shiraz, Iran2- Nanobiology and Nanomedicine Research Centre, Shiraz University of Medical sciences, Shiraz, Iran
http://orcid.org/0000-0001-6943-477X
- Maryam Owjfard3 3- Basic Science Research Lab, Shiraz University of Medical Sciences, Shiraz, Iran
- Majid Reza Farokhi1
DOI:
https://doi.org/10.22100/jkh.v13i3.1925Keywords:
Curcumin, 6-OHDA, MAPKs, ERK, SH-SY5Y.Abstract
Introduction: Curcumin, a natural phenolic yellow chemical derived from turmeric, has been found to exert some protective effects in animal and cellular models of Parkinson's disease (PD). The present study aimed to examine whether curcumin prevents the effect of 6-OHDA in human neuroblastoma cells and MAPKs signaling.
Methods: SH-SY5Y cells were treated with 6-OHDA (50 µM) for 24 h. The effect of curcumin (2 and 2.5 µM) on cellular viability (MTT assay) and MAPKs (ERK, JNK and p38) (wester blot assay) was evaluated.
Results: 6-OHDA caused significant reduction of neuronal viability and p-ERK decrement. The results indicate that curcumin can partially protected against 6-OHDA induced cell death and p-ERK decline.
Conclusion: These finding suggest that ERK restoration is related to the protective effect of curcumin against 6-OHDA in human neuroblastoma cells.
References
Driver JA, Logroscino G, Gaziano JM, Kurth T. Incidence and remaining lifetime risk of Parkinson disease in advanced age. Neurology 2009;72:432-8. doi:10.1212/01.wnl.0000341769.50075.bb
Morgante L, Morgante F, Moro E, Epifanio A, Girlanda P, Ragonese P, et al. How many parkinsonian patients are suitable candidates for deep brain stimulation of subthalamic nucleus? Results of a questionnaire. Parkinsonism & related disorders 2007;13:528-31. doi:10.1016/j.parkreldis.2006.12.013
Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: the Indian solid gold. Advances in experimental medicine and biology 2007;595:1-75. doi:10.1007/978-0-387-46401-5_1
Srimal RC, Dhawan BN. Pharmacology of diferuloyl methane (curcumin), a non-steroidal anti-inflammatory agent. The Journal of pharmacy and pharmacology 1973;25:447-52.
Masuda T, Hidaka K, Shinohara A, Maekawa T, Takeda Y, Yamaguchi H. Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin. J Agric Food Chem 1999;47:71-7.
Sui Z, Salto R, Li J, Craik C, Ortiz de Montellano PR. Inhibition of the HIV-1 and HIV-2 proteases by curcumin and curcumin boron complexes. Bioorg Med Chem 1993;1:415-22.
Kim JH, Nam SW, Kim BW, Kim WJ, Choi YH. Astaxanthin improves the proliferative capacity as well as the osteogenic and adipogenic differentiation potential in neural stem cells. Food Chem Toxicol 2010;48:1741-5. doi:10.1016/j.fct.2010.04.002
Wang XS, Zhang ZR, Zhang MM, Sun MX, Wang WW, Xie CL. Neuroprotective properties of curcumin in toxin-base animal models of Parkinson’s disease: a systematic experiment literatures review. BMC complementary and alternative medicine 2017;17:412. doi:10.1186/s12906-017-1922-x
Zbarsky V, Datla KP, Parkar S, Rai DK, Aruoma OI, Dexter DT. Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and fisetin in a 6-OHDA model of Parkinson's disease. Free radical research 2005;39:1119-25. doi:10.1080/10715760500233113
Wang J, Du XX, Jiang H, Xie JX. Curcumin attenuates 6-hydroxydopamine-induced cytotoxicity by anti-oxidation and nuclear factor-kappa B modulation in MES23.5 cells. Biochemical pharmacology 2009;78:178-83. doi:10.1016/j.bcp.2009.03.031
Rajeswari A, Sabesan M. Inhibition of monoamine oxidase-B by the polyphenolic compound, curcumin and its metabolite tetrahydrocurcumin, in a model of Parkinson's disease induced by MPTP neurodegeneration in mice. Inflammopharmacology 2008;16:96-9. doi:10.1007/s10787-007-1614-0
Jaisin Y, Thampithak A, Meesarapee B, Ratanachamnong P, Suksamrarn A, Phivthong-Ngam L, et al. Curcumin I protects the dopaminergic cell line SH-SY5Y from 6-hydroxydopamine-induced neurotoxicity through attenuation of p53-mediated apoptosis. Neuroscience letters 2011;489:192-6. doi:10.1016/j.neulet.2010.12.014
Meesarapee B, Thampithak A, Jaisin Y, Sanvarinda P, Suksamrarn A, Tuchinda P, et al. Curcumin I mediates neuroprotective effect through attenuation of quinoprotein formation, p-p38 MAPK expression, and caspase-3 activation in 6-hydroxydopamine treated SH-SY5Y cells. Phytotherapy research: PTR 2014;28:611-6. doi:10.1002/ptr.5036
Song JX, Shaw PC, Wong NS, Sze CW, Yao XS, Tang CW, et al. Chrysotoxine, a novel bibenzyl compound selectively antagonizes MPP(+), but not rotenone, neurotoxicity in dopaminergic SH-SY5Y cells. Neuroscience letters 2012;521:76-81. doi:10.1016/j.neulet.2012.05.063
Cheung YT, Lau WK, Yu MS, Lai CS, Yeung SC, So KF, et al. Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research. Neurotoxicology 2009;30:127-35. doi:10.1016/j.neuro.2008.11.001
Schule B, Pera RA, Langston JW. Can cellular models revolutionize drug discovery in Parkinson's disease? Biochimica et biophysica acta 2009;1792:1043-51. doi:10.1016/j.bbadis.2009.08.014
Forno LS. Neuropathology of Parkinson's disease. Journal of neuropathology and experimental neurology 1996;55:259-72. doi:10.1016/j.parkreldis.2017.07.033
Xing C, Peng Y, Chang R, Yin Y, Xie Z. Effects of insulin-like growth factor-1 on okadaic acid-induced apoptosis in SH-SY5Y cells. Cell biology international 2005;29:803-8. doi:10.1016/j.cellbi.2005.04.012
Ljungdahl A, Hokfelt T, Jonsson G, Sachs C. Autoradiographic demonstration of uptake and accumulation of 3H-6-hydroxydopamine in adrenergic nerves. Experientia 1971;27:297-9.
Hwang O. Role of oxidative stress in Parkinson's disease. Experimental neurobiology 2013;22:11-7. doi:10.5607/en.2013.22.1.11
Khopde SM, Priyadarsini KI, Guha SN, Satav JG, Venkatesan P, Rao MN. Inhibition of radiation-induced lipid peroxidation by tetrahydrocurcumin: possible mechanisms by pulse radiolysis. Biosci Biotechnol Biochem 2000;64:503-9.
Jaroonwitchawan T, Chaicharoenaudomrung N, Namkaew J, Noisa P. Curcumin attenuates paraquat-induced cell death in human neuroblastoma cells through modulating oxidative stress and autophagy. Neuroscience letters 2017;636:40-7. doi:10.1016/j.neulet.2016.10.050
Ganguli M, Chandra V, Kamboh MI, Johnston JM, Dodge HH, Thelma BK, et al. Apolipoprotein E polymorphism and Alzheimer disease: The Indo-US Cross-National Dementia Study. Arch Neurol 2000;57:824-30.
Khatri DK, Juvekar AR. Neuroprotective effect of curcumin as evinced by abrogation of rotenone-induced motor deficits, oxidative and mitochondrial dysfunctions in mouse model of Parkinson's disease. Pharmacology, biochemistry, and behavior 2016;150-151:39-47. doi:10.1016/j.pbb.2016.09.002
Song S, Nie Q, Li Z, Du G. Curcumin improves neurofunctions of 6-OHDA-induced parkinsonian rats. Pathology, research and practice 2016;212:247-51. doi:10.1016/j.prp.2015.11.012
Chu CT, Levinthal DJ, Kulich SM, Chalovich EM, DeFranco DB. Oxidative neuronal injury. The dark side of ERK1/2. European journal of biochemistry / FEBS 2004;271:2060-6. doi:10.1111/j.1432-1033.2004.04132.x
Bogoyevitch MA, Court NW. Counting on mitogen-activated protein kinases--ERKs 3, 4, 5, 6, 7 and 8. Cellular signalling 2004;16:1345-54. doi:10.1016/j.cellsig.2004.05.004
Roskoski R. ERK1/2 MAP kinases: structure, function, and regulation. Pharmacological research: the official journal of the Italian Pharmacological Society 2012;66:105-43. doi:10.1016/j.phrs.2012.04.005
Subramaniam S, Unsicker K. ERK and cell death: ERK1/2 in neuronal death. FEBS J 2010;277:22-9. doi:10.1111/j.1742-4658.2009.07367.x
Ren Y, Jiang H, Yang F, Nakaso K, Feng J. Parkin protects dopaminergic neurons against microtubule-depolymerizing toxins by attenuating microtubule-associated protein kinase activation. The Journal of biological chemistry 2009;284:4009-17. doi:10.1074/jbc.M806245200
Zhuang S, Schnellmann RG. A death-promoting role for extracellular signal-regulated kinase. The Journal of pharmacology and experimental therapeutics 2006;319:991-7. doi:10.1124/jpet.106.107367
Zhu JH, Kulich SM, Oury TD, Chu CT. Cytoplasmic aggregates of phosphorylated extracellular signal-regulated protein kinases in Lewy body diseases. The American journal of pathology 2002;161:2087-98. doi:10.1016/s0002-9440(10)64487-2
Numakawa T, Matsumoto T, Numakawa Y, Richards M, Yamawaki S, Kunugi H. Protective Action of Neurotrophic Factors and Estrogen against Oxidative Stress-Mediated Neurodegeneration. Journal of toxicology 2011;2011:405194. doi:10.1155/2011/405194
Stanciu M, Wang Y, Kentor R, Burke N, Watkins S, Kress G, et al. Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures. J Biol Chem 2000;275:12200-6.
Ramachandiran S, Huang Q, Dong J, Lau SS, Monks TJ. Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells. Chem Res Toxicol 2002;15:1635-42.
Wang X, Martindale JL, Holbrook NJ. Requirement for ERK activation in cisplatin-induced apoptosis. The Journal of biological chemistry 2000;275:39435-43. doi:10.1074/jbc.M004583200
Zhuang S, Yan Y, Daubert RA, Han J, Schnellmann RG. ERK promotes hydrogen peroxide-induced apoptosis through caspase-3 activation and inhibition of Akt in renal epithelial cells. American journal of physiology Renal physiology 2007;292:F440-7. doi:10.1152/ajprenal.00170.2006
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
