Curcumin prevents 6-OHDA Induced Cell Death and ERK disruption in Human Neuroblastoma Cells

Authors

  • Maryam Moosavi1,2 1- Shiraz Neuroscience Research Center, Shiraz University of Medical sciences, Shiraz, Iran2- Nanobiology and Nanomedicine Research Centre, Shiraz University of Medical sciences, Shiraz, Iran orcid http://orcid.org/0000-0001-6943-477X
  • Maryam Owjfard3 3- Basic Science Research Lab, Shiraz University of Medical Sciences, Shiraz, Iran
  • Majid Reza Farokhi1

DOI:

https://doi.org/10.22100/jkh.v13i3.1925

Keywords:

Curcumin, 6-OHDA, MAPKs, ERK, SH-SY5Y.

Abstract

Introduction: Curcumin, a natural phenolic yellow chemical derived from turmeric, has been found to exert some protective effects in animal and cellular models of Parkinson's disease (PD). The present study aimed to examine whether curcumin prevents the effect of 6-OHDA in human neuroblastoma cells and MAPKs signaling.

Methods: SH-SY5Y cells were treated with 6-OHDA (50 µM) for 24 h. The effect of curcumin (2 and 2.5 µM) on cellular viability (MTT assay) and MAPKs (ERK, JNK and p38) (wester blot assay) was evaluated.

Results: 6-OHDA caused significant reduction of neuronal viability and p-ERK decrement. The results indicate that curcumin can partially protected against 6-OHDA induced cell death and p-ERK decline.

Conclusion: These finding suggest that ERK restoration is related to the protective effect of curcumin against 6-OHDA in human neuroblastoma cells.

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Published

2018-11-19

Issue

Section

Original Article(s)

How to Cite

Curcumin prevents 6-OHDA Induced Cell Death and ERK disruption in Human Neuroblastoma Cells. (2018). Knowledge and Health in Basic Medical Sciences, 13(3), 1-7. https://doi.org/10.22100/jkh.v13i3.1925

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