The effect of the mechanical unloading of lower limb on MST1 and Atrogin1 gene expression in Plantaris and soleus muscles of Wistar rats
Authors
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Abdolreza Kazemi 1,2
1- Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. 2- Department of Physical Education and Sport Sciences, Faculty of humanity and literature, Vali E ASR University, Rafsanjan, IR Iran.
http://orcid.org/0000-0003-1082-6335
DOI:
https://doi.org/10.22100/jkh.v13i3.2004Keywords:
Mechanical unloading, Skeletal muscle mass, MST1, Atrogin 1Abstract
Introduction: The mammalian Ste20-like kinase (MST) is involved in the activation of the ubiquitin pathway ligase atrogin1 in denervation atrophy. The aim of the present study was the study of changes in MST1 mRNA and Atrogin1 mRNA gene expression in the muscles of male Wistar rats with mechanical unloading of hind limb muscles.
Methods: In the present study 10 Wistar rats (4-6 month) divided into 2 groups randomly (control (N=5) and unloading of the hind limb (N=5) groups). The practical group unloaded of hind limb for 14 days and the control group spent the normal life during this period. After 14 days, the rats were sacrificed and their muscles and Plantaris were collected and after weighing the muscle, they were kept in liquid nitrogen.
Results: The results of this study showed that after 14 days of lower limb suspension, the relative weight of both soleus and plantar muscles decreased but this reduction was not significant in Plantaris skeletal muscle. In addition, the expression of genes MST1 mRNA (P=0.021) significantly increased and also atrogen-1 mRNA (P=0.056) in soleus muscle increased. Also, MST1 mRNA (P=0.071) and atrogen-1 mRNA (P=0.33) in Plantaris muscle increased insignificantly. Comparison of the genes expression in two skeletal muscles showed that the expression of MST1 mRNA in soleus more than Plantaris, on the other hand, expression of atrogen-1 mRNA in Plantaris increased more than soleus muscle.
Conclusion: According to the results of this study, it can be claimed that MST1 can be one of the main targets of controlling atrophy in conditions mechanical unloading, such as hospitalization and space travel. However, the lower targets of MST1 can be different in two muscles.
References
Tidball JG. Mechanical signal transduction in skeletal muscle growth and adaptation. J Appl Physiol 2005;98(5):1900-8. doi:10.1152/japplphysiol.01178.2004
Andersson DC, Betzenhauser MJ, Reiken S, Meli AC, Umanskaya A, Xie W, et al. Ryanodine receptor oxidation causes intracellular calcium leak and muscle weakness in aging. Cell Metab 2011;14(2):196-207. doi:10.1016/j.cmet.2011.05.014
Hulmi JJ, Silvennoinen M, Lehti M, Kivelä R, Kainulainen H. Altered Redd1, myostatin, and Akt/Mtor/Foxo/Mapk signaling in streptozotocin-induced diabetic muscle atrophy. Am J Physiol Endocrinol Metab 2012;302(3):E307-15. doi:10.1152/ajpendo.00398.2011
Rodriguez J, Vernus B, Chelh I, Cassar-Malek I, Gabillard JC, Hadj Sassi A. Myostatin and the skeletal muscle atrophy and hypertrophy signaling pathways. Cell Mol Life Sci 2014;71(22):4361-71. doi:10.1007/s00018-014-1689-x
Giger JM, Bodell PW, Zeng M, Baldwin KM, Haddad F. Rapid muscle atrophy response to unloading: pretranslational processes involving MHC and actin. J Appl Physiol 2009;107(4):1204-12. doi:10.1152/japplphysiol.00344.2009
Adams GR, Caiozzo VJ, Baldwin KM. Skeletal muscle unweighting: spaceflight and ground-based models. J Appl Physiol 2003;95(6):2185-201. doi:10.1152/japplphysiol.00346.2003
Fitts RH, Riley DR, Widrick JJ. Physiology of a microgravity environment invited review: microgravity and skeletal muscle. J Appl Physiol 2000;89(2):823-39. doi:10.1152/jappl.2000.89.2.823
Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, Bauerlein R, et al. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nat Cell Biol 2001;3(11):1014-9. doi:10.1038/ncb1101-1014
Cohen S, Brault JJ, Gygi SP, Glass DJ, Valenzuela DM, Gartner C, et al. During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation. The Journal of Cell Biology 2009;185(6):1083-1095. doi:10.1083/jcb.200901052
Gomes MD, Lecker SH, Jagoe RT, Navon A, Goldberg AL. Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy. Proc Natl Acad Sci U S A 2001;98(25):14440-5. doi:10.1073/pnas.251541198
Bodine SC, Latres E, Baumhueter S, Lai VK, Nunez L, Clarke BA, et al. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 2001;294(5547):1704-8. doi:10.1126/science.1065874
Sandri M, Sandri C, Gilbert A, Skurk C, Calabria E, Picard A, et al. Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy. Cell 2004;117(3):399-412.
Zhao J, Brault JJ, Schild A, Cao P, Sandri M, Schiaffino S, et al. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. Cell Metab 2007;6(6):472-83. doi:10.1016/j.cmet.2007.11.004
Mammucari C, Milan G, Romanello V, Masiero E, Rudolf R, Del Piccolo P, et al. FoxO3 controls autophagy in skeletal muscle in vivo. Cell Metab 2007;6(6):458-71. doi:10.1016/j.cmet.2007.11.001
Carvalho RF1, Castan EP, Coelho CA, Lopes FS, Almeida FL, Michelin A, et al. Heart failure increases atrogin-1 and MuRF1 gene expression in skeletal muscle with fiber type-specific atrophy. J Mol Histol 2010;41(1):81-7. doi:10.1007/s10735-010-9262-x
Reed SA, Senf SM, Cornwell EW, Kandarian SC, Judge AR. Inhibition of IkappaB kinase alpha (IKKα) or IKKbeta (IKKβ) plus forkhead box O (Foxo) abolishes skeletal muscle atrophy. Biochem Biophys Res Commun 2011;405(3):491-6. doi:10.1016/j.bbrc.2011.01.059
Fanzani A, Conraads VM, Penna F, Martinet W. Molecular and cellular mechanisms of skeletal muscle atrophy: an update. J Cachexia Sarcopenia Muscle 2012;3(3):163-79. doi:10.1007/s13539-012-0074-6
Chopard A, Hillock S, Jasmin BJ. Molecular events and signalling pathways involved in skeletal muscle disuse-induced atrophy and the impact of countermeasures. J Cell Mol Med 2009;13(9B):3032-50. doi:10.1111/j.1582-4934.2009.00864.x
Graves JD, Draves KE, Gotoh Y, Krebs EG, Clark EA. Both phosphorylation and caspase-mediated cleavage contribute to regulation of the Ste20-like protein kinase Mst1 during CD95/Fas-induced apoptosis. J Biol Chem 2001;276(18):14909-15. doi:10.1074/jbc.M010905200
Zhao B, Li L, Guan KL. Hippo signaling at a glance. J Cell Sci 2010;123(Pt 23):4001-6. doi:10.1242/jcs.069070
Watt KI, Judson R, Medlow P, Reid K, Kurth TB, Burniston JG. Yap is a novel regulator of C2C12 myogenesis. Biochem Biophys Res Commun 2010;393(4):619-24. doi:10.1016/j.bbrc.2010.02.034
You B, Yan G, Zhang Z, Yan L, Li J, Ge Q. Phosphorylation of cardiac troponin I by mammalian sterile 20-like kinase 1. Biochem J 2009;418(1):93-101. doi:10.1042/BJ20081340
Wei B, Dui W, Liu D, Xing Y, Yuan Z, Ji G. MST1, a key player, in enhancing fast skeletal muscle atrophy. BMC Biol 2013;11:12. doi:10.1186/1741-7007-11-12
Deschenes MR, Maresh CM, Crivello JF, Armstrong LE, Kraemer WJ, Covault J. The effects of exercise training of different intensities on neuromuscular junction morphology. J Neurocytol 1993;22(8):603-15.
Martin TP, Edgerton VR, Grindeland RE. Influence of spaceflight on rat skeletal muscle. J Appl Physiol 1988;65(5):2318-25. doi:10.1152/jappl.1988.65.5.2318
Edgerton VR, Zhou MY, Ohira Y, Klitgaard H, Jiang B, Bell G, et al. Human fiber size and enzymatic properties after 5 and 11 days of spaceflight. J Appl Physiol 1995;78(5):1733-9. doi:10.1152/jappl.1995.78.5.1733
Zhang BT, Yeung SS, Liu Y, Wang HH, Wan YM, Ling SK, et al. The effects of low frequency electrical stimulation on satellite cell activity in rat skeletal muscle during hindlimb suspension. BMC cell biol 2010;11:87. doi:10.1186/1471-2121-11-87
Reed SA Sandesara PB, Senf SM, Judge AR. Inhibition of FoxO transcriptional activity prevents muscle fiber atrophy during cachexia and induces hypertrophy. FASEB J 2012;26(3):987-1000. doi:10.1096/fj.11-189977
Gomes MD, Lecker SH, Jagoe RT, Navon A, Goldberg AL. Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy. Proc Natl Acad Sci U S A 2001;98(25):14440-5. doi:10.1073/pnas.251541198
Hanson AM, Kittilson JD, Martin LE, Sheridan MA. Environmental estrogens inhibit growth of rainbow trout (Oncorhynchus mykiss) by modulating the growth hormone-insulin-like growth factor system. Gen Comp Endocrinol 2014;196:130-8. doi:10.1016/j.ygcen.2013.11.013
Zhang P, Chen X, Fan M. Signaling mechanisms involved in disuse muscle atrophy. Med Hypotheses 2007;69(2):310-21. doi:10.1016/j.mehy.2006.11.043
Lehtinen MK, Yuan Z, Boag PR, Yang Y, Villén J, Becker EB, et al. A conserved MST-FOXO signaling pathway mediates oxidative-stress responses and extends life span. Cell 2006;125(5):987-1001. doi:10.1016/j.cell.2006.03.046
Lin Y, Khokhlatchev A, Figeys D, Avruch J. Death-associated protein 4 binds MST1 and augments MST1-induced apoptosis. J Biol Chem 2002;277(50):47991-8001. doi:10.1074/jbc.M202630200
Ohira Y, Nomura T, Kawano F, Sato Y, Ishihara A, Nonaka I. Effects of nine weeks of unloading on neuromuscular activities in adult rats. J Gravit Physiol 2002;9(2):49-59.
Ohira Y, Kawano F, Wang XD, Sudoh M, Iwashita Y, Majima HJ, et al. Irreversible morphological changes in leg bone following chronic gravitational unloading of growing rats. Life Sci 2006;79(7):686-94. doi:10.1016/j.lfs.2006.02.022
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