The Effect of Aerobic Exercise and Chlorpyrifos Toxin on the Expression of Spa, Cop1 Genes and Lung Tissue in Male Rats

Abstract

Introduction: Due to the severe side effects of using or exposure to chlorpyrifos toxin (CPF) on the lungs and respiratory system, in this study, the effects of aerobic exercise on improving the effects of CPF poisoning on the level of lung tissue changes and gene expression in Rats were examined.

Methods: Sixteen Wistar rats were divided into 6 groups: healthy control, dose 1-poisoned control group, dose 3- poisoned control group, dose 1-poisoned training group, dose 3-poisoned training group, and DMSO control. CPF was injected intraperitoneally in two different doses to the target groups. After intoxication, exercise was given to the target groups according to a specific exercise program. Histological assessments, evaluation of oxidative stress markers (SOD and MDA), and expression changes of Spa and Cop-1 genes in all study groups were performed.

Results: Results from histological evaluations showed that the rate of mucosal destruction of lung tissue was increased in the control groups poisoned by dose 1 and dose 3 of CPF (P=0.001). SOD and MDA levels were reduced and enhanced, respectively, in poisoned control groups. After aerobic exercise, the severity of tissue damage was significantly reduced. SOD and MDA levels were significantly increased and decreased, respectively, in trained groups. At the molecular level, it was also observed that the expression of Spa and Cop-1 genes in the CPF-poisoned control group was significantly reduced, which increased significantly compared to the control groups after aerobic exercise.

Conclusion: According to the results of this study, aerobic exercise seems to play an effective role in reducing tissue damage due to oxidative stress and regulating the expression of tissue-specific genes by reducing oxidative stress induced by CPF.