Fabrication and Evaluation of Decellularized Amnion Membrane-Gelatin Scaffold for using in Tissue Engineering Applications
Authors
-
Shiva Asadpour
- Dept. of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran. - Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
https://orcid.org/0000-0001-5574-7142
-
Elham Zendedel
- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
https://orcid.org/0000-0001-8698-0326
- Maryam Anjomshoa - Dept. of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
- Mehrab Askariyanpoor - Dept. of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
- Akram KaramiDehkordi - Dept. of Midwifery, School of Nursing and Midwifery Shahrekord University of Medical Sciences, Shahrekord, Iran.
DOI:
https://doi.org/10.22100/jkh.v18i4.3188Keywords:
Aminon membrane, decellularization, gelatin, tissue engineeringAbstract
Introduction: Decellularization techniques are widely used in the fabrication of suitable scaffolds for the regeneration of damaged tissues in order to overcome the problem of lack of donor tissues. Decellularized extracellular matrix offers several advantages over synthetic materials, including preservation of natural microenvironmental characteristics. Amnion membrane has features such as availability, cheapness, low immunogenicity, anti-inflammatory and regerative effects. On the other hand, gelatin is a natural and biocompatible polymer that has good interactions with cell adhesion and growth. Therefore, in this study, gelatin-decellularized amniotic membrane scaffolds were fabricated and evaluated for use in tissue engineering.
Materials and methods: At first, the amniotic membrane was decellularized, and then the degree of decellularization was evaluated by hematoxylin-eosin staining. Then, the scaffolds were fabricated. After the construction of the scaffolds, the physical, chemical and mechanical characterization of the scaffolds was done. Then, the mesenchymal stem cells derived from the endometrium of the uterus were cultured on the scaffolds and the biocompatibility of the scaffolds was evaluated
Results: The results showed that the amnion tissue was decellularized. The surface of the scaffolds was rough in the SEM images. Due to the presence of hydrophilic gelatin polymer in the structure of the scaffolds, all the scaffolds had hydrophilic properties. The constructed scaffolds had water absorption or swelling. The results of the mechanical properties of the scaffolds showed that the scaffolds have adequate mechanical strength. The results of the growth and biocompatibility test showed that the proliferation of stem cells derived from uterine endometrium on the scaffolds was more than the control group.
Conclusion: The scaffolds fabricated using amino-gelatin curtain have the potential to be used in tissue engineering applications.
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