Evaluation of BDNF Expression Level in Male Wistar Rats After Six Weeks of Swimming

Authors

DOI:

https://doi.org/10.22100/jkh.v19i3.3259

Keywords:

Brain-drived neurotrophic factor, Hippocampus, Water temperature

Abstract

Introduction: Aerobic exercises enhance cognitive performance. Given the crucial role of BDNF expression in this enhancement, the present study investigated the effects of six weeks of swimming in both cold water and normal temperature on cognitive performance and the expression levels of BDNF in brain tissue.

Methods: Twenty-five male Wistar rats (8 weeks old, weighing 250 ± 30 g) were obtained from the Pasteur Institute. After a two-week acclimatization period in a standard laboratory environment, the rats participated in an initial cognitive function test. They were randomly divided into three groups: one for swimming in cold water, one for swimming in normal temperature water, and a control group. The two exercise groups then followed the exercise protocol for six weeks. After that, the Real-time PCR method was used to measure the BDNF gene expression. A one-way ANOVA test was also used to analyze the findings.

Results: The results showed that cognitive performance enhanced significantly after six weeks of swimming when compared to the control group (P<001). Additionally, BDNF expression levels in brain tissue were significantly higher in the two training groups than in the control group (P<002). Furthermore, the increase in BDNF levels in the cold water swimming group was statistically higher than that observed in the normal temperature swimming group (P<0.04).

Conclusion: It seems that changes in BDNF expression play a role in enhancing cognitive performance as a result of sports training, and swimming in cold water may provide an effective stimulus to enhance the training's effects on BDNF.

References

Wrann CD WJ, Salogiannnis J, Laznik-Bogoslavski D, Wu J, Ma D, Lin JD, Greenberg ME, Spiegelman BM. . Exercise induces hippocampal BDNF through a PGC-1alpha/ FNDC5 pathway. Cell Metab. 2013;18(5):649–59.

Sun G QS, Jiang Q, Liu K, Li B, Li M, Zhao L, Zhou Z, von Deneen KM, Liu Y. Hyperthermia-induced disruption of functional connectivity in the human brain network. PLoS One. 2013;8(4):e61157.

Smiley-Oyen AL LK, Francois SJ, Kohut ML, Ekkekakis P. Exercise, fitness, and neurocognitive function in older adults: The “selective improvement” and “cardiovascular fitness” hypotheses. Ann Behav Med. 2008;36(3):280–91.

Chaddock L HC, Buck SM, Cohen NJ. Aerobic fitness and executive control of relational memory in preadolescent children. Med Sci Sports Exerc. 2011;43(2):344–9.

Chaddock L EK, Prakash RS, Voss MW, Van- Patter M, Pontifex MB, Hillman CH, Kramer AF. A functional MRI investigation of the association between childhood aerobic fitness and neurocognitive control. Biol Psychol. 2012;89(1):260–8.

Raine LB L, Saliba BJ, Chaddock-Heyman L,Hillman CH, Kramer AF. The influence of childhood aerobic fitness on learning and memory. PLoS One. 2013;8(9):e72666.

Wu CT PM, Raine LB, Chaddock L, Voss MW, Kramer AF, Hillman CH. Aerobic fitness and response variability in preadolescent children performing a cognitive control task. Neuropsychology. 2011;25(3):333–41.

Hansen DM HS, Lambourne K, Lee J, Donnelly JE. Linear/nonlinear relations of activity and fitness with children’s academic achievement. Med Sci Sports Exerc. 2014;46(12):2279–85.

Voss MW HS, Prakash RS, Erickson KI, Alves H, Chaddock L, Szabo AN, Mailey EL, Wojcicki TR, White SM, et al. . The influence of aerobic fitness on cerebral white matter integrity and cognitive function in older adults: Results of a one-year exercise intervention. Hum Brain Mapp. 2013;34(11):2972–85.

Erickson KI WA, Sutton BP, Prakash RS, Voss MW, Chaddock L, Szabo AN, Mailey EL, White SM, Wojcicki TR, et al. . Beyond vascularization: aerobic fitness is associated with N-acetylaspartate and working memory. Brain Behav. 2012;2(1):32–41.

Ahmadiasl N AH, H€anninen O. Effect of exercise on learning, memory and levels of epinephrine in rats’ hippocampus. J Sports Sci Med. 2003;2(3):106–9.

Gordon BA RE, Brumback CR, Lee Y, Elavsky S, Konopack JF, McAuley E, Kramer AF, Colcombe S, Gratton G, et al. . Neuroanatomical correlates of aging, cardiopulmonary fitness level, and education. Psychophysiology. 2008;45(825–38):825–38.

Clark RE BN, Squire LR. Hippocampus and remote spatial memory in rats. Hippocampus. 2005;15(2):260–72.

Cotman C.W. CC, Berchtold N.C. Exercise: a behavioral intervention to enhance brain health and plasticity. Trends Neurosci. 2002;25(6):295–301.

Meiri AKaN. Brain-Derived Neurotrophic Factor Is Critically Involved in Thermal-Experience-Dependent Developmental Plasticity. The Journal of Neuroscience. 2006;26(15):3899-907.

Erickson KI VM, Prakash RS, Basak C, Szabo A, Chaddock L, KimJS, Heo S, Alves H,White SM, et al. Exercise training increases size of hippocampus and improves memory. Proc Natl Acad Sci USA. 2011;108(7):3017–22.

Rasmussen P BP, Adser H, Pedersen MV, Leick L, Hart E, Secher NH, Pedersen BK, Pilegaard H. Evidence for a release of brain-derived neurotrophic factor from the brain during exercise. Exp Physiol. 2006;94(10):1062–9.

Christopher W. Collins RJS, Matthew W. S. Heesch, and Dustin R. Slivka. The effect of environmental temperature on exercise-dependent release of brain-derived neurotrophic factor. TEMPERATURE. 2017;4(3):305–13.

Rasmussen P BP, Adser H, Pedersen MV, Leick L, Hart E, Secher NH, Pedersen BK, Pilegaard H. . Evidence for a release of brain-derived neurotrophic factor from the brain during exercise. . Exp Physiol. 2009;4(10):1062–9.

Klein AB WR, Santini MA, Clemmensen C, Ettrup A, Rios M, Knudsen GM, Aznar S. . Blood BDNF concentrations reflect brain-tissue BDNF levels across species. Int J Neuropsychopharmacol. 2011;14(3):347–53.

Nakagawa T O-KM, Sugaru E, Yamanaka M, Taiji M, Noguchi H Brain-derived neurotrophic factor (BDNF) regulates glucose and energy metabolism in diabetic mice. Diabetes Metab Res Rev. 2002;18(3):185–91.

AE J. Modulation of carbohydrate and fat utilization by diet, exercise and environment. Biochem Soc Trans. 2003;31(Pt 6):1270–3.

SR MR. The neuropharmacological and neurochemical basis of place learning in the Morris water maze. Brain Res Brain Res Rev. 1993;18(1):33-49.

Petzinger GMF BEA, G.; Holschneider, D.P.; Wood, R.; Walsh, J.P.; Lund, B.; Meshul, C.; Vuckovic, M.; Jakowec,M.W The role of exercise in facilitating basal ganglia function in Parkinson’s disease. Neurodegener Dis Manag. 2011;1:157-70.

Sara Shams MA SA-S, Hamid Rajabi , Katsuhiko Suzuki. . Swimming in cold water upregulates genes involved in thermogenesis and the browning of white adipose tissues. Comparative Biochemistry and Physiology. 2023;Part B 265:110834.

Kirk-Sanchez NJM, E.L. Physical exercise and cognitive performance in the elderly: Current perspectives. Clin Interv Aging. 2013;9:51–62.

Kim GHI, K.; Kwon, H.; Seo, S.W.; Ye, B.S.; Cho, H.; Noh, Y.; Lee, J.M.; Kim, S.T.; Park, S.E.; et al. . Higher Physical Activity Is Associated with Increased Attentional Network Connectivity in the Healthy Elderly. Front Aging Neurosci. 2016;8:198.

Spaniol J. Event-related fMRI studies of episodic encoding and retrieval: Meta-analyses using activation likelihood estimation. Neuropsychologia. 2009;47:1765–79.

Bherer LE, K.I.; Liu-Ambrose, T. A Review of the Effects of Physical Activity and Exercise on Cognitive and Brain Functions in Older Adults. J Aging Res. 2013;2013:1-8.

Cotman CWB, N.C.; Christie, L.A. . Exercise builds brain health: Key roles of growth factor cascades and inflammation Trends Neurosci. 2007;30:464–72.

Park HP, M.M. Neurotrophin regulation of neural circuit development and function. Nature reviews Neuroscience. 2013;14(7-23).

Egan MFK, M.; Callicott, J.H.; Goldberg, T.E.; Kolachana, B.S.; Bertolino, A.; Zaitsev, E.; Gold, B.; Goldman, D.; Dean, M.; et al. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal

function. Cell 2003;112:257–69.

Kesslak JPS, Vannarith Choi, James Cotman, Carl W. Gomez-Pinilla, Fernando. Learning upregulates brain-derived neurotrophic factor messenger ribonucleic acid: A mechanism to facilitate encoding and circuit maintenance? Behavioral Neuroscience,. 1998;112(4):1012-9.

Qin WS, L.; Cao, J.; Peng, Y.; Collier, L.; Wu, Y.; Creasey, G.; Li, J.; Qin, Y.; Jarvis, J.; et al. . The central nervous system (CNS)-independent anti-bone-resorptive activity of muscle contraction and the underlying molecular and cellular signatures. J Biol Chem. 2013;288:13511–21.

Choi SHB EC, Z.K.; Lee, S.W.; Pulli, B.; Clemenson, G.D.; Kim, E.; Rompala, A.; Oram, M.K.; Asselin, C.;, al. e.. . Combined adult neurogenesis and BDNF mimic exercise effects on cognition in an Alzheimer’s mouse model. Science. 2018;36(eaan8821).

D N. Short-term memory and long-term memory are still different. Psychol Bull. 2017;143:992–1009.

Colcombe SJE KIS, P.E.; Kim, S.J.; Prakash, R.; McAuley, E. Aerobic Exercise Training Increases Brain Volume in Aging Humans. J Gerontol Ser A Biol Sci Med Sci. 2006;61:1166–70.

Quindry JCF BA. Exercise Preconditioning as a Cardioprotective Phenotype. Am J Cardiol. 2021;148:8–15.

Claudia Camerino* EC, Roberta Caloiero, Adriano Fonzino, Mariarosaria Carratù, Marcello D. Lograno and Domenico Tricarico. Evaluation of Short and Long Term Cold Stress Challenge of Nerve Grow Factor, Brain-Derived Neurotrophic Factor, Osteocalcin and Oxytocin mRNA Expression in BAT, Brain, Bone and Reproductive Tissue of Male Mice Using Real-Time PCR and Linear Correlation Analysis. frontiers in physiology. 2018;8(1101):1-8.

Mustroph ML CS, Desai SC, Cay EB, DeYoung EK, Rhodes JS. . Aerobic exercise is the critical variable in and enriched environment that increases hippocampal neurogenesis and water maze learning in male C57BL/6J mice. Neuroscience. 2012;219:62–71.

Published

2024-10-23

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Original Article(s)

How to Cite

Evaluation of BDNF Expression Level in Male Wistar Rats After Six Weeks of Swimming. (2024). Knowledge and Health in Basic Medical Sciences, 19(3), page:10-17. https://doi.org/10.22100/jkh.v19i3.3259

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