Protective Effects of Hydroalcoholic Extract of Salvia Officinalis on Anxiety-Like Behaviors Induced by Intraventricular Administration of Streptozotocin in Male Rats

Abstract

Introduction: The present study aimed to investigate the effect of Salvia officinalis on anxiety-like behaviors triggered by intracerebroventricular injection of streptozotocin in male Wistar rats.

Methods: The animals were divided into four groups: control, vehicle, streptozotocin, and streptozotocin with Salvia officinalis treatment, which included dosages of 50, 25, and 100 mg/kg. The control group received no treatment. The Vehicle group received saline intraperitoneally and intracerebroventricularly. The streptozotocin group and the streptozotocin + Salvia officinalis treatment groups received saline intraperitoneally and hydroalcoholic extract of Salvia officinalis, respectively, for 14 days. Intracerebroventricular cannulas were then implanted into the animals' brains. One week after recovery, the streptozotocin group was administered streptozotocin (3 mg/kg), while the streptozotocin+ Salvia officinalis group was administered Salvia officinalis extract intraventricularly. Anxiety-like behavior was evaluated at two time points: 5 minutes and one week after streptozotocin administration, using the Elevated Plus Maze.

Conclusion: The plant extract of Salvia officinalis shows a dose-dependent protective effect against anxiety induced by intracerebroventricular administration of streptozotocin in rats.