Comparison of the Antiviral Activity of Aqueous and Alcoholic Extracts of Cyanobacteria Collected From Iranian South Oil

Authors

  • Soheila Sadat Mirhoseini Ardakani1 1- Dept. of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran.
  • Mehrouz Dezfulian2 2- Dept. of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran.
  • Neda Soltani3 3- Dept. of Petroleum Microbiology, Research Institute of Applied Science, ACECR, Tehran, Iran.

DOI:

https://doi.org/10.22100/jkh.v10i2.607

Keywords:

Cyanobacteria, Antiviral, Methanol extract, Aqueous extract

Abstract

Introduction:The antimicrobial properties of cyanobacteria was recently highlighted. The aim of this study was to evaluate the activity and antiviral properties of cyanobacteria collected from oil-polluted areas of southern Iran.

Methods: In the present study, aqueous and methanolic extracts of five species ofcyanobacteria were prepared. Cytotoxic effects ofcyanobacteria on (lymphoblast cell line) LCL and Hela cell lineswere evaluated. Using MTT assay and medium only served as a negative control group. Hela and LCL cells also infected by human papilloma virus (HPV) and Epstein-Barr virus (EBV) respectively and were exposed to the cyanobacteria extracts to examine the anti-viral effects of extracts. DNA was extracted and the viral gene encoding envelope was also carried out by PCR. The samples were assessed in order to examinethat viruses are capable of successfully infecting and lysing embedded colonies. The data were analyzed using Two-way ANOVA tests.

Results: The results indicated that cyanobacteria extract had cytotoxic effects on LCL and HeLa cell lines. In addition viral load gene was significantly decreased in treated cells.

Conclusion: According to the significant antiviral effects of alcoholic and aqueous extracts of cyanobacteria. These extracts may used for a variety of viral therapies.

References

Mundt S, Teuscher E. Blue-green algae asa source of pharmacologically-active compound. Pharmazie 1988;43:805-816.

Vijaya Baskara, Sethubathi G, Ashok Prabu V. Antibacterial activity of cyanobacterial species from adirampattinam coast, southeast coast of palk bay. Curr Res J Biol Sci 2010;2:24-26.

Kulik MM. The potential for using cyanobacteria (blue-green algae) and algae in the biological control of plant pathogenic bacteria and fungi. European J Plant path 1995;101:585-599.

Mtolera P, Semesi A. Antimicrobial activity of extracts from six green algae from Tanzania. Curr. Trends Mar. Bot. Res. East Afr. Reg 1996;211-217.

Browitzka MA. Microalgae as sources of pharmaceuticals and other biologically active compounds. J Appl Phycol 1995;7:3-15.

Keritlow S, Mundt S, Lindequist U. Cyanobacteria- a potential source of new biologically active substances. J Biotechnol 1999;70:60-63.

Dahms H-U, Xu Y, Pfeiffer C. Antifouling potential of cyanobacteria: a mini-review. Biofouling 2006;22;317-327.

Ozdemir G, Karabay NU, Dalay MC, Pazarbasi B. Antibacterial activity of volatile component and various extracts of spirulina platensis. Phytother Res 2004;18:754-757.

Mundt S, Kreitlow S, Jansen R. Fatty acids with antibacterial activity from the cyanobacterium Oscillatoria redekei HUB 051. JAppl Phycol 2003;15:263-267.

Shanab SMM. Bioactive allelo-chemical compounds from oscillatoria species (egyptian isolates). Int J Agric Biol 2007;9: 617-621.

Abed RMM, Dobretsov S, Sudesh K. Applications of cyanobacteria in biotechnology. J Appl Microbiol 2009;106:1-12.

Carmichael WW. Cyanobacteria secondary metabolites--the cyanotoxins. J Appl Bacterio 1992;72:445-459.

Patterson GML, Baker KK, Baldwin CL, Bolis CM, Caplan FR, Larsen LK, et al. Antiviral activity of cultured blue-green algae (Cyanophyta). Journal of Phycology 1993;29:125-130.

Mundt S, Kreitlow S, Nowotny A, Effmert U. Biochemical and pharmacological investigations of selected cyanobacteria. Int J Hyg Environ Health 2001;203:327-334.

Glombitza KW, Koch M. Secondary metabolites of pharmaceutical potential. In: Cress Well RC, Rees TAV, Shah. Algal and Cyanobacterial. Book of Biotechnology, Longman Scientific Technical, Harlow, UK. 1989;161-238.

Schwartz RE, Hirsch CF, Sesin DF. Pharmaceuticals from cultured algae. Journal of Industrial Microbiology 1990;5:113-124.

El-Sheekh MM, Osman ME, Dyab M, Amer MS. Production and characterization of antimicrobial active substance from the cyanobacterium nostoc muscorum toxicol. Pharmacol 2006;21:42-50.

Noaman NH, Fattah A, Khaleafa M, Zaky SH. Factors affecting antimicrobial activity of Synechococcus leopoliensis. Microbiological Research 2004;159:395-402.

Singh S, Bhushan K, Banerjee U. Bioactive compounds from cyanobacteria and microalgae: An overview. Critical Reviews in Biotechnology 2005;25:73-95.

Bechelli J, Coppage M, Rosell K, Liesveld J. Cytotoxicity of algae extracts on normal andmalignant cells. Leukemia Research and Treatment 2010;19:35-37.

Kok YY, Chu WL, Phang SM, Mohamed SM, Naidu R, Lai PJ, et al. Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells. J Zhejiang Univ Sci B 2011;12:335-45.

Schaeffer DJ, Krylov VS. Anti-HIV activity of extracts and compounds from algae and cyanobacteria. Ecotoxicol Environ Saf 2000;45:208-27.

Published

2015-03-18

Issue

Section

Original Article(s)

How to Cite

Comparison of the Antiviral Activity of Aqueous and Alcoholic Extracts of Cyanobacteria Collected From Iranian South Oil. (2015). Knowledge and Health in Basic Medical Sciences, 10(2), Page:39-46. https://doi.org/10.22100/jkh.v10i2.607

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