Evaluation of Specific Anti-ESAT-6 Antibody in Diagnosis of Bovine Tuberculosis

Authors

  • Roghaieh Aghazadeh1 1- Dept. of Microbiology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
  • Majid Tebianian2 2- Dept. of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Mehdi Mahdavi3 3- Dept. of Immunology, Pasteur Institute of Iran, Tehran, Iran.

DOI:

https://doi.org/10.22100/jkh.v10i4.939

Keywords:

سل گاوي, مايكوباكتريوم, ESAT-6, الايزا, آنتي بادي

Abstract

Introduction: Bovine tuberculosis (BTB) is a zoonotic disease that affected domestic and wild animals, and humans. It is caused by Mycobacterium bovis (M. bovis) and has a wide host range. The effective control of BTB paramount importance and this can be achieved through the use of accurate and comprehensive diagnostic tests.  The most widely used methods to detect BTB are the skin test and in vitro gamma interferon assay which do not detect anergic animals, but serological tests such as ELISA have been found promising in ancillary tuberculosis diagnosis. The overall aim was to study M. tuberculosis ESAT-6 recombinant protein as a target for serological assays in the detection of antibodies to BTB.

Methods: Serum samples were collected from suspected animals where M. tuberculosis was confirmed at postmortem examination by clinicians and positive skin test. Also, control samples were collected from BTB free bovines. Circulating specific antibody levels were measured by an enzyme-linked immunosorbent assay using purified recombinant proteins of ESAT-6.

Results: The results show a mild satisfactory agreement with the skin test reflecting in 95.23 sensitivity and 81.81 Specificityin serum diluted to 1/100.

Conclusion: This study demonstrated that ELISA with the use of the esat-6 antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB.

References

Okada M, Kobayashi K. Recent progress in mycobacteriology. Kekkaku 2007;82:783-99.

Tabbara KF. Tuberculosis. Curr Opin Ophtalmol 2007;18:493-501.

Dye C, Garnett GP, Sleeman K, Williams BG. Prospects for worldwide tuberculosis control under the World Health Organization (WHO) DOTS strategy. Lancet 1998;352:1886-91.

WHO Report 2006: Global tuberculosis control; Surveillance, Planning, Financing.p.1-250.

Smith I. Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence. Clin Microbiol Rev 2003;16:463-96.

Baumann S, Nasser Eddine A, Kaufmann SH. Progress in tuberculosis vaccine development. Current Opinion in Immunology 2006;18:438-48.

Wing Wal Yew, Glovanni B. Migliori and christoph Lange, Advances in the diagnosis of tuberculosis. Respirology 2010;l: 220-40.

MV, Thacker TC, et al. Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle. Clin Vaccine Immunol 2011;18:1882-8.

Perkins MD, Conde MB, Martins M, Kritski AL. Serologic diagnosis of tuberculosis using a simple commercial multiantigen assay. CHEST Journal 2003;123:107-12.

Vordermeier H, Whelan A, Cockle P, Farrant L, Palmer N, Hewinson R. Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle. Clin Diagn Lab Immunol 2001;8:571-8.

Coates A, Allen B, Hewitt J, Ivanyi J, Mitchison D. Antigenic diversity of mycobacterium tuberculosis and mycobacterium bovis detected by means of monoclonal antibodies. The Lancet 1981; 318:167-9.

Singh KK, Dong Y, Patibandla SA, McMurray DN, Arora VK, Laal S. Immunogenicity of the mycobacterium tuberculosis PPE55 (Rv3347c) protein during incipient and clinical tuberculosis. Infect Immun 2005;73:5004-14.

Andersen P, Andersen AB, Sørensen A, Nagai S. Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice. The Journal of Immunology 1995;154:3359-72.

Lyashchenko KP, Pollock JM, Colangeli R, Gennaro ML. Diversity of antigen recognition by serum antibodies in experimental bovine tuberculosis. Infect Immun 1998;66:5344-9.

Vordermeier HM,Chambers MA, Cockle PJ, Whelan AO, Simmons J, Hewinson RG. Correlation of ESAT-6-specific gamma interferon production with pathology in cattle following mycobacterium bovis BCG vaccination against experimental bovine tuberculosis. Infect Immun 2002;70:3026-32.

Kwok HF, Scott CJ, Snoddy P, Buick RJ, Johnston JA, Olwill SA. Expression and purification of diagnostically sensitive mycobacterial mycobacterium bovis antigens and profiling of their humoral immune response in a rabbit model. Res Vet Sci 2010;89:41-7.

Vutla JB, Chandran D, Veerasami M, Sugumar P, Shahana PV, Das D, et al. Cloning, expression and purification of ESAT-6 and CFP-10 and their use in the detection of IFN-γ responses in tuberculosis infected cattle. Biotechnol Bioinf Bioeng 2011;1:255-264.

Greenaway C, Lienhardt C, Adegbola R, Brusasca P, McAdam K, Menzies D. Humoral response to mycobacterium tuberculosis antigens in patients with tuberculosis in the Gambia. The International Journal of Tuberculosis and Lung Disease 2005;9:1112-9.

Sang-Nae cho. Current issues on molecular and immunological diagnosis of tuberculosis. Yonsei Med J 2007;48:347-59.

Schluger NW, Burzynski J. Recent advances in testing for latent TB. Chest 2010;138:1456-63.

Jolley ME, Nasir MS, Surujballi OP, Romanowska A, Renteria TB, De La Mora A, et al. Fluorescence polarization assay for the detection of antibodies to mycobacterium bovis in bovine sera. Vet Microbio 2007;120:113-21.

Sartain MJ, Slayden RA, Singh KK, Laal S, Belisle JT. Disease state differentiation and identification of tuberculosis biomarkers via native antigen array profiling. Mol Cell proteomics 2006;5:2102-13.

Okkels LM, Andersen P. Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. Journal of Bacteriology 2004;186:2487-91.

Brodin P, Rosenkrands I, Andersen P, Cole ST, Brosch R. ESAT-6 proteins: protective antigens and virulence factors? Trends in Microbiology 2004;12:500-8.

Pollock JM, Andersen P. Predominant recognition of the ESAT-6 protein in the first phase of infection with Mycobacterium bovis in cattle. Infect Immun 1997;65:2587-92.

WHO report 2007: global tuberculosis control: surveillance, planning, financing: World Health Organization. 2007.

Vutla JB, Chandran D, Veerasami M, Sugumar P, Shahana PV, Das D, et al. Cloning, expression and purification of ESAT-6 and CFP-10 and their use in the detection of IFN-γ responses in tuberculosis infected cattle. Biotechnol Bioinf Bioeng 2011;1:255-64.

Bekmurzayeva A, Sypabekova M, Kanayeva D. Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis. Tuberculosis 2013;93:381-8.

Jacobson RH. Validation of serological assays for diagnosis of infectious diseases, revue scientifique et technique. Revue scientifique et technique (International Office of Epizootics) 1998;17:469-526.

Ivanyi J, Krambovitis E, Keen M. Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis. Clin Exp Immunol 1983;54:337-45.

Ma Y, Wang Y-M, Daniel T. Enzyme-linked immunosorbent assay using Mycobacterium tuberculosis antigen 5 for the diagnosis of pulmonary tuberculosis in China. The American review of Respiratory Disease 1986;134:1273-5.

Waters WR, Buddle BM, Vordermeier HM, Gormley E, Palmer

Published

2015-09-29

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Section

Original Article(s)

How to Cite

Evaluation of Specific Anti-ESAT-6 Antibody in Diagnosis of Bovine Tuberculosis. (2015). Knowledge and Health in Basic Medical Sciences, 10(4), Page:72-78. https://doi.org/10.22100/jkh.v10i4.939

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