القای تمایز بن‌یاخته‌های مزانشیمی خون بندناف انسان به رده یاخته‌های شبه‌هپاتوسیتی در شرايط آزمایشگاهي

نویسندگان

  • Masoumeh Ghaderi
  • Abdolamir Allameh
  • Masoud Soleimani
  • Mahdi Forozande Moghadam

DOI::

https://doi.org/10.22100/jkh.v7i2.79

کلمات کلیدی:

خون بندناف، بن‌یاخته‌های مزانشیمی، تمایز، هپاتوسیت

چکیده

مقدمه: بازسازی بافت‌ در محیط آزمایشگاه با استفاده از یاخته‌های بنیادی، یکی از موضوعات مورد علاقه محققان می‌باشد. بافت کبد نیز از این امر مستثنا نیست. هدف از این مطالعه، تولید یاخته‌های شبه‌هپاتوسیتی از بن‌یاخته‌های مزانشیمی خون بندناف می‌باشد.

مواد و روش‌ها: بن‌ياخته‌هاي مزانشيمي، از خون بندناف (با کسب رضايت از مادران) جدا شدند و سپس ايمنوفنوتيپ بن‌ياخته‌هاي جداشده با فلوسايتومتري و توانايي تمايز آن‌ها با آزمون‌هاي تمايز در محيط آزمایشگاه بررسي شدند. درنهايت، بيان مارکرهاي اختصاصي کبد به‌واسطه واکنش‌هاي زنجيره‌اي پليمراز- معکوس (RT-PCR) آناليز شدند.

نتایج: بن‌ياخته‌هاي مزانشیمی (Mesenchymal Stem Cells) جداشده از خون بندناف، توانايي تمايز به سويه مزودرمي را داشتند. چنانچه اين بن‌ياخته‌ها در شرايط مناسب کشت داده شوند، قادر به القای ژن‌هاي کبدي؛ مانند: آلبومين (Alb)، آلفافيتوپروتئين (AFP)، سيتوکراتين‌هاي 18 و 19 (18CK، 19CK) و آلفا-1-آنتي‌تريپسين (AA) هستند. همچنين در پي القاي تمايز به‌سمت هپاتوسيت‌ها، بن‌ياخته‌هاي مزانشيمي خون بندناف، پروتئين‌هاي آلبومين و آلفافيتوپروتئين را توليد مي‌کنند.

نتيجه‌گیری: بن‌ياخته‌هاي مزانشيمي خون بندناف، توانايي تمايز به سويه‌ هپاتوسيتي را دارند؛ لذا ابزار مناسبي در درمان‌هاي ياخته‌اي با هدف بازسازي بافت کبد به‌شمار‌مي‌روند.

مراجع

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Kazemnejad S, Allameh A, Gharehbaghian A, Soleimani M, Amirizadeh N, Jazayeri M. Efficient replacing of fetal bovine serum with human platelet releasate during propagation and differentiation of human bone marrow-derived mesenchymal stem cells to functional hepatocytes-like cells. Vox Sang 2008;95:149-58.

Haynesworth SE, Baber MA, Caplan AI. Cell surface antigens on human bone marrow-derived mesenchymal stem cells are detected by monoclonal antibodies. Bone 1992;13:69-80.

Gang EJ, Hong SH, Jeong JA, Hwang SH, Kim SW, Yang IH, et al. In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells. Biochem Bioph Res Co 2004;321:102-8.

Javazon EH, Beggs K, Flake A. Mesenchymal stem cells: paradoxes of passaging. Exp Hematol 2004;32:414-25.

Park PC, Selvarajah S, Bayani J, Zielenska M, Squire JA. Stem cell enrichment approaches. Semin Cancer Biol 2007;17:257-64.

Gudoitti JE, Bregerie O, Robert A, Debey P, Brechot C, Desdoutes C. Liver cell poliplooidization: a pivotal role for binuclear hepatocytes. J Biol Chem 2003;278:19095-101.

Alison MR, Pouslom R, Otto WR, Vig P, Brittan M. Recipies for adult stem cell plasticity:fusion cuisine or readymade. J Clin Pathol 2004;57:113-20.

Khoramroudi A, Nasiri S, Amiri I. Avaluating biochemical factors of in vitro differentiated hepatocyte like cells derived from cord blood stem cells. Armaghane Danesh 1998;3:23-34.[Persian].

Raufi A, Amini A, Azadbakht M, Aboozari M, Fathi F. In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells identified by cellular uptake of Indocyanine Green. blood 2010;7:33-40.[Persian].

Wright N, Samuelson L, Walkup MH, Chandrasekaran P, Gerber DA. Enreachment of a bipotent hepatic progenitor cell from native liver tissue. Biochem Biophys Res Commun 2008;36:367-72.

Khuu DN, Najimi M, Sokal EM. Epithelial cells with hepatobiliary phenotype: Is it another stem cell candidate for healthy adult human liver. World J Gastroenterol 2007;13:1554-60.

Kinoshita T, Miyajima A. Cytokine regulation of liver development. Biochim Biophys Acta 2002;1592:303-12.

Fujino H, Hiramatsu H, Tsuchiya A, Niwa A, Noma H, Shiota M, et al. Human cord blood cd34+ cells develop into hepatocytes in the livers of nod/scid/gc nnull mice through cell fusion. FASEB J 2007;21:3499-510.

Ek M, Soderdahl T, Kuppers-Munther B, Edsbagge J, Andersson TB, Bjorquist P, et al. Expression of drug metabolizing enzymesiin hepatocyte-like cells derived from human embryonic stem cells. Biochem Pharmacol 2007;74:496-503.

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2012-07-03

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