Investigation of the Effect of Cardiac Rehabilitation Program on Peripheral Blood Mononuclear Cells ABCA1 Gene Expression in Myocardial Infractions Patient

Authors

  • Alireza Hajighasemi1 1- Dept. of Exercise Physiology, Alborz campus, Tehran University, Tehran, Iran.
  • Ali Asghar Ravasi2 2- Dept. of Exercise Physiology, College of Physical Education and Sport Sciences, Tehran University, Tehran, Iran.
  • Mohamadreza Kordi3 3- Dept. of Exercise Physiology, College of Physical Education and Sport Sciences, Tehran University, Tehran, Iran.
  • Amir Rashidlamir4 4- Dept. of Exercise Physiology, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Akhtar Ghorghi5 5- Dept. of Exercise Physiology, Shahrood University, Shahrood, Iran.

DOI:

https://doi.org/10.22100/jkh.v11i4.1523

Keywords:

Moderate resistance training, ABCA1 gene, Reverse cholesterol transport

Abstract

Introduction: Atherosclerotic heart disease is a major important cause of mortality in most countries. ABCA1 transporter is responsible for making and forming of HDL particles and therefore probably plays a crucial role in prevention of atherosclerosis. The purpose of this study was to assess the effect of rehabilitation training on ABCA1 gene expression of peripheral blood mononuclear cells (PBMN).

Methods: Twenty three myocardial infractions patients were randomly selected and assigned into two groups; control (N=11) and experimental (N=12). Moderate resistance training was conducted by experimental group for 60 minutes at three sessions for eight weeks with 40-60% of one repetition maximum (1RM). Blood sample of subjects were collected before and after training. PBMN cells were separated and m-RNA purification was performed by real–time PCR. Data was analyzed by ANCOVA in SPSS software (significant leve equal 0.05).

Results: Results showed that expression of PBMN ABCA1 m-RNA was significantly increased with moderate resistance training in myocardial infractions patient group. There was no significant differences in mean of HDL-C, LDL-C (P>0.05).

Conclusion: As result that moderate resistance training increases the expression of ABCA1 gene on PBMN cells of MI patient, improves the function of reverse cholesterol transfer, and probably prevents repetition of Ischemic.

References

Rashidlamir A. Investigation of the effect of aerobic and resistance exercises on peripheral blood mononuclear cells ABCG1 gene expression in female athletes. JSSU 2012;20:1-9.

Shokrallahnia roshan A, Sadeghi H, Shirani Sh, Nejatian M. Effects of strength training and cardiac rehabilitation programs on the biomechanical parameters of blood flow velocity and blood flow rate and its relation with arterial stiffness index in brachial and femoral arteries with coronary artery bypass grafting patients (CABG). Tavanbakhshi Journals 2013;14:1-7.

Gelissen IC, Harris M, Rye KA, Quinn C, Brown AJ, Kockx M, et al. ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I. Arterioscler Thromb Vasc Biol 2006;26:534-40. doi: 10.1161/01.ATV.0000200082.58536.e1

Yvan-Charvet L, Wang N, Tall AR. Role of HDL, ABCA1, and ABCG1 transporters in cholesterol efflux and immune responses. Arterioscler Thromb Vasc Biol 2010;30:139-43. doi: 10.1161/ATVBAHA.108.179283

Butcher L, Thomas A, Backx K, Roberts A, Webb R, Morris K. Low-intensity exercise exerts beneficial effects on plasma lipids via PPARgamma. Med Sci Sports Exerc 2008;40:1263-70. doi: 10.1249/MSS.0b013e31816c091d

Von Eckardstein A, Nofer JR, Assmann G. High density lipoproteins and arteriosclerosis role of cholesterol efflux and reverse cholesterol transport. Arterioscler Thromb Vasc Biol 2001;21:13-27.

Masuda D, Yamashita S. Very high levels of High-Density lipoprotein cholesterol and cardiovascular events in Japanese population. J Atheroscler Thromb 2016;23:771-2. doi: 10.5551/jat.ED049

Singaraja RR, Fievet C, Castro G, James ER, Hennuyer N, Clee SM, et al. Increased ABCA1 activity protects against atherosclerosis. J Clin Invest 2002;110:35-42.

Li AC, Glass CK. PPAR-and LXR-dependent pathways controlling lipid metabolism and the development of atherosclerosis. J Lipid Res 2004;45:2161-73. doi: 10.1194/jlr.R400010-JLR200

Pollock ML, Franklin BA, Balady GJ, Chaitman BL, Fleg JL, Fletcher B, et al. Resistance exercise in individuals with and without cardiovascular disease benefits, rationale, safety, and prescription an advisory from the committee on exercise, rehabilitation, and prevention, council on clinical cardiology, american heart association. Circulation 2000;101:828-33. doi: 10.1161/01.CIR.101.7.828

Ghanbari-Niaki A, Ghanbari-Abarghooi S, Rahbarizadeh F, Zare-Kookandeh N, Gholizadeh M, Roudbari F, et al. Heart ABCA1 and PPAR-α genes expression responses in male rats: effects of high intensity treadmill running training and aqueous extraction of black crataegus-pentaegyna. Res Cardiovasc Med 2013;2:153-9. doi: 10.5812/cardiovascmed.13892

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods 2001;25:402-8. doi: 10.1006/meth.2001.1262

Demina EP, Miroshnikova VV, Rodygina TI, Kurianov PS, Vinogradov AG, Denisenko AD, et al. ABCA1 gene expression in peripheral blood lymphocytes and macrophages in patients with atherosclerosis. Mol Biol 2011;45:258-93.

Larrede S, Quinn CM, Jessup W, Frisdal E, Olivier M, Hsieh V, et al. Stimulation of cholesterol efflux by LXR agonists in cholesterol-loaded human macrophages is ABCA1-dependent but ABCG1-independent. Arterioscler Thromb Vasc Biol 2009;29:1930-6. doi: 10.1161/ATVBAHA.109.194548

Fatone C, Guescini M, Balducci S, Battistoni S, Settequattrini A, Pippi R, et al. Two weekly sessions of combined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetes mellitus and metabolic syndrome. J Endocrinol Invest 2010;33:489-95. doi: 10.1007/BF03346630

Kenaga M, Higaki Y, Saku K, Uehara Y. High-density lipoprotein mimetics: a therapeutic tool for atherosclerotic diseases. J Atheroscler Thromb 2016;23:385-94. doi: 10.5551/jat.33720

Khabazian BM1, Ghanbari-Niaki A, Safarzadeh-Golpordesari Ar, Ebrahimi M, Rahbarizadeh F, Abednazari H. Endurance training enhances ABCA1 expression in rat small intestine. Eur J Appl Physiol 2009;107:351-8. doi: 10.1007/s00421-009-1133-3

Rashid S, Genest J. Effect of obesity on high-density lipoprotein metabolism. Obesity 2007; 15:2875-88. doi: 10.1038/oby.2007.342

Soumian S, Albrecht C, Davies AH, Gibbs RG. ABCA1 and atherosclerosis. Vasc Med 2005;10:109-19.

Shearman J, Micklewright D, Hardcastle J, Hamlin M, Draper N. The effect of physical activity on serum lipids, lipoprotein, and. apolipoproteins. Arch Exerc Health Dis 2010;1:43-9.

Curtiss LK1, Valenta DT, Hime NJ, Rye KA. What is so special about apolipoprotein a-i in reverse cholesterol transport?. Arterioscler Thromb Vasc Biol 2006;26:12-9. doi: 10.1161/01.ATV.0000194291.94269.5a

Khabazian B, Ghanbari-Niakki A, Hosseini-Khakhak A, Rahbarizadeh F, Hedayati M, Jabari Noghabi M. The effect of short term endurance training on the expression of hepatic abca1 and reverse cholesterol transport in male wistar rats. Iranian Journal of Endocrinology and Metabolism 2010;11:568-75.

Yvan-Charvet L, Pagler TA, Seimon TA, Thorp E, Welch CL, Witztum JL, et al. ABCA1 and ABCG1 protect against oxidative stress–induced macrophage apoptosis during efferocytosis. Circ Res 2010;106:1861-9. doi: 10.1161/CIRCRESAHA.110.217281

Ghanbari-Niaki A, Saghebjoo M, Hedayati M. A single session of circuit-resistance exercise effects on human peripheral blood lymphocyte ABCA1 expression and plasma HDL-C level. Regul Pept 2011;166:42-7. doi: 10.1016/j.regpep.2010.08.001

Ghanbari-Niaki A. Treadmill exercise training enhances ATP-binding cassette protein-A1 (ABCA1) expression in male rats’ heart and gastrocnemius muscles. Int J Endocrinol Metab 2010;8:206-10.

Ghorbanian B, Ravassi A, Kordi MR, Hedayati M. The effects of rope training on lymphocyte ABCA1 expression, plasma ApoA-I and HDL-c in boy adolescents. Int J Endocrinol Metab 2013;11:76-81. doi: 10.5812/ijem.8178

Buttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells. J Appl Physiol 2007;102:26-36. doi: 10.1152/japplphysiol.00066.2006

Hoang A, Tefft C, Duffy SJ, Formosa M, Henstridge DC, Kingwell BA, et al. ABCA1 expression in humans is associated with physical activity and alcohol consumption. Atherosclerosis 2008;197:197-203. doi: 10.1016/j.atherosclerosis.2007.03.017

Garzon P, Sheppard R, Eisenberg MJ, Schechter D, Lefkovits J, Goudreau E, et al. Comparison of event and procedure rates following percutaneous transluminal coronary angioplasty in patients with and without previous coronary artery bypass graft surgery (the ROSETTA Registry). Am J Cardiol 2002;89:251-6.

Ozcan H, Oztekin PS, Zergeroglu AM, Ersöz G, Ficicilar H, Ustuner E. Doppler ultrasound evaluation of the structural and hemodynamic changes in the brachial artery following two different exercise protocols. Diagn Interv Radiol 2006;12:80-4.

Koba S, Ayaori M, Vto-Kondo H, et al. Beneficial Effects of Exercise-Based Cardiac Rehabilitation on High-Density lipoprotein- Mediated cholesterol Efflux Capacity in patients with Acute Coronary Syndrome. J Atheroscler Thromb 2016;23:865-77. doi: 10.5551/jat.34454

Rashidlamir A, Ghanbari-Niaki A, Saadatnia A. The effect of eight weeks of wrestling and wrestling technique based circuit trainingon lymphocyte ABCA1 gene expression and plasma apolipoprotein A-I. World Journal of Sport Sciences 2011;4:144-50.

Mostafavi S, Saeidi M, Heidari H. Effects of a comprehensive cardiac rehabilitation program on the quality of life of patients with cardio-vascular diseases. Journal of Research in Rehabilitation Sciences 2012;7:516-24.

Borg GA. Physical performance and perceived exertion: Gleerup Lund; 1962.

Published

2017-02-05

Issue

Section

Original Article(s)

How to Cite

Investigation of the Effect of Cardiac Rehabilitation Program on Peripheral Blood Mononuclear Cells ABCA1 Gene Expression in Myocardial Infractions Patient. (2017). Knowledge and Health in Basic Medical Sciences, 11(4), page:23-29. https://doi.org/10.22100/jkh.v11i4.1523

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